园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 205-209.

• 研究论文 • 上一篇    下一篇


汪俏梅;郭得平; Kyikyi Win; 袁 晶

  1. (浙江大学园艺系,杭州310029)
  • 收稿日期:2003-10-20 修回日期:2004-01-13 出版日期:2004-04-25 发布日期:2004-04-25

Effect of 1-MCP on Delaying the Senescence of Broccoli and Its PossiblePhysiological Mechanism

Wang Qiaomei;Guo Deping;Kyikyi Win; Yuan Jing

  1. (Department of Horticulture,Zhejiang University,Hangzhou 310029,China)
  • Received:2003-10-20 Revised:2004-01-13 Online:2004-04-25 Published:2004-04-25

摘要: 研究了1—甲基环丙烯(1-MCP)对青花菜衰老的影响。结果表明,0~20 µL/L的1-MCP在
20℃下处理青花菜花球6 h,能延长2O 藏条件下苛仡菜的货架寿命,延缓花蕾中营养成分叶绿素、类胡萝卜素和维生素c等的降解,其中以2.5 µL/L处 的效果最佳。进一步研究还表明2.5 µL/L 1-MCP处理抑制花蕾的呼吸速率和乙烯释放速率,行使 藏期 花蕾的蛋白质含量升高;丙二醛(MDA)含量下降,使超氧化物歧化酶(SOD)和过氧化氧酶(CAT)的活性升高,过氧化物酶(POD)的活性下降,同时,SDS-PAGE分析表明,这一处理使花球中醇溶性蛋白和水溶性蛋白的组分和含量发生明显变化

关键词: 青花菜, 1—甲基环丙烯, 贮藏, 衰老, 蛋白质, SDS-PAGE

Abstract: The effect of 1-methylcyclopropene (1-MCP) on the senescence of broccoli was studied.The results showed that the shelf life of broccoli was extended and the degradations of chlorophyl 1-carotenoid and vitamin C delayed by exposure to 1-MCP at concentration range of 0—20 µL/L for 6 h at room temperature (20 ℃)followed by storage at 20 ℃ 1-MCP at optimal concentration of 2.5 µL/L was used to investigate the possible mechanism of 1-MCP in delaying the senescence. The respiration rate and ethylene production of florets were inhibited, the protein content of florets, the activities of SOD and CAT increased, while the MDA content and the activity of POD decreased during storage by exposure to 2.5 µL/L 1-MCP.SDS-PAGE analysis further showed that the composition and content of both the alcohol-soluble and water-soluble proteins changed after 1-MCP treatment.

Key words: Broccoli, 1-methylcyclopropene, Storage, Senescence, Protein, SDS-PAGE