茄子全基因组SNP标记的开发

doi:10.16420/j.issn.0513-353x.2023-0586

摘要/Abstract

摘要：

茄子（Solanum melongena L.）是中国重要的茄科蔬菜之一，其具有十分丰富的遗传多样性，对茄子种质资源进行精准评价是利用茄子种质资源的基础。利用40份茄子（Solanum melongena L.）种质资源的重测序数据，筛选了268个SNP位点用于五引物扩增受阻突变体系（penta-primer amplification refractory mutation system，PARMS）-SNP标记的开发，成功获得原创性的120个PARMS-SNP标记。利用这些标记对224份茄子高代自交系进行基因分型，并进一步进行群体结构、PCA主成分分析和遗传树分析。结果表明该224份材料可以分为两个组。同时根据多态性信息含量（polymorphic information content，PIC）值筛选了48个核心PARMS-SNP标记，并且构建了能够将224份材料进行区分的指纹图谱。

关键词: 茄子, SNP标记, 遗传多样性分析, 指纹图谱

Abstract:

Eggplant（Solanum melongena L.）is one of the important vegetables in the Solanaceae family in China. It has a very rich genetic diversity，and accurate evaluation of eggplant germplasm resources is the basis for using eggplant germplasm resources. In this study，the resequencing data of 40 eggplant germplasm resources were used to screen 268 SNPs for the development of PARMS-SNP （penta-primer amplification refractory mutation，five-prime amplification refractory mutation system） markers，and finally successfully obtained 120 original PARMS-SNP markers. Using 120 SNP markers for gene typing of 224 high-generation self-crossing lines of eggplant，the population structure，PCA （principal component analysis），and genetic tree results showed that the 224 materials could be divided into two groups. At the same time，48 core PARMS-SNP markers were selected based on the PIC（polymorphic information content）value，and a fingerprint map that can distinguish between 224 materials was constructed.

Key words: eggplant, SNP marker, genetic diversity, fingerprint

肖熙鸥, 聂珩, 林文秋, 吴彩玉. 茄子全基因组SNP标记的开发[J]. 园艺学报, 2025, 52(1): 88-100.

XIAO Xi’ou, NIE Heng, LIN Wenqiu, WU Caiyu. Development of Whole-Genome SNP Markers of Eggplant[J]. Acta Horticulturae Sinica, 2025, 52(1): 88-100.

图/表 9

参考文献 33

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果型 Fruit shape	果色 Fruit color	编号 No.
卵圆 Oval	紫色 Purple	8、9、13
椭圆 Ellipse	紫色 Purple	14、15、17、59
圆 Round	紫色 Purple	125
棒形 Long cylindrical	白色 White	34、40、44、45、47，48、51、54、57、67、123
	绿色 Green	39、53、55、106
	紫色 Purple	1 ~ 7、10 ~ 12、16、18 ~ 33、35 ~ 38、41 ~ 43、46、49、50、52、56、58、60 ~ 66、68 ~ 105、107 ~ 122、124、126 ~ 224

果型 Fruit shape	果色 Fruit color	编号 No.
卵圆 Oval	紫色 Purple	8、9、13
椭圆 Ellipse	紫色 Purple	14、15、17、59
圆 Round	紫色 Purple	125
棒形 Long cylindrical	白色 White	34、40、44、45、47，48、51、54、57、67、123
	绿色 Green	39、53、55、106
	紫色 Purple	1 ~ 7、10 ~ 12、16、18 ~ 33、35 ~ 38、41 ~ 43、46、49、50、52、56、58、60 ~ 66、68 ~ 105、107 ~ 122、124、126 ~ 224

ID	染色体 Chr	位置 Position	参考碱基，变异 Ref，Alt	X引物序列 Sequence of primer X	Y引物序列 Sequence of primer Y	Z引物序列 Sequence of primer Z
SNP 06	01	44620003	A，G	GAAGGTGACCAAGTTCATGCTTACACATGATCTTAAATTAGTGGCCA	GAAGGTCGGAGTCAACGGATTTACACATGATCTTAAATTAGTGGCCG	CAGATAGTGACACCATATTTTTAATGCAAATATG
SNP 07	01	53995173	T，A	GAAGGTGACCAAGTTCATGCTCTAGCCACGTGGAGGAGGATAT	GAAGGTCGGAGTCAACGGATTCTAGCCACGTGGAGGAGGATAA	CTATAATCATGTAGGGACAGGCGATC
SNP09	01	59104419	T，C	GAAGGTGACCAAGTTCATGCTCTGAGGACCTAACAACATTGTGCTT	GAAGGTCGGAGTCAACGGATTTGAGGACCTAACAACATTGTGCTC	GGCCTTAGTTTGGGTCTTGACAAACT
SNP24	01	83357891	A，C	GAAGGTGACCAAGTTCATGCTGTTCACTGTATTATAGCATGTTTGACA	GAAGGTCGGAGTCAACGGATTGTTCACTGTATTATAGCATGTTTGACC	GCGTATGTCTCAATAATCAATGTAAAATCAG
SNP25	01	83358171	C，T	GAAGGTGACCAAGTTCATGCTCGAGTCCTACATGTCGATCCTC	GAAGGTCGGAGTCAACGGATTTCGAGTCCTACATGTCGATCCTT	TTGGGCATGGCCATCGAAGCTC
SNP26	01	84072334	G，C	GAAGGTGACCAAGTTCATGCTTATGCCTACATGATGTTACCATGTAG	GAAGGTCGGAGTCAACGGATTTATGCCTACATGATGTTACCATGTAC	TCGGTTCCTACATGGTAACAGAGCT
SNP44	01	122998346	T，C	GAAGGTGACCAAGTTCATGCTTATTCCACCTCATTTTGAAACTCAGAAT	GAAGGTCGGAGTCAACGGATTTTCCACCTCATTTTGAAACTCAGAAC	TTGAGGAAGGAACCTAAGATTCATTTGC
SNP48	02	9305544	G，T	GAAGGTGACCAAGTTCATGCTACGGTCTAAACTAAGGGCCTAG	GAAGGTCGGAGTCAACGGATTTCACGGTCTAAACTAAGGGCCTAT	GGGTTCCCCTTGCGCTGTGTCA
SNP56	02	74119173	A，G	GAAGGTGACCAAGTTCATGCTAAATTCTCTTGCCAGACTTATTGAAAATATTA	GAAGGTCGGAGTCAACGGATTTTCTCTTGCCAGACTTATTGAAAATATTG	TGTTTGTATGTGGATGATTTGATCTTGATAG
SNP59	02	82393242	A，G	GAAGGTGACCAAGTTCATGCTTATAACCAAAGTGAAACATCAAAACTTCAA	GAAGGTCGGAGTCAACGGATTATAACCAAAGTGAAACATCAAAACTTCAG	TAATACTGGATTGTGTAAGTCTTCAACTTTG
SNP61	02	83239126	A，G	GAAGGTGACCAAGTTCATGCTGGTAAGCATTAATCAGGCCTGTCA	GAAGGTCGGAGTCAACGGATTGTAAGCATTAATCAGGCCTGTCG	GAAGTTTTAGTCCTCTACATACATAAAACTTAG
SNP72	03	25452216	C，T	GAAGGTGACCAAGTTCATGCTTCTTGTTTCTCCTTTACCTCCACC	GAAGGTCGGAGTCAACGGATTATCTTGTTTCTCCTTTACCTCCACT	TCCTTGTGCATAATGGATCTACATCATC
SNP84	03	76800537	G，A	GAAGGTGACCAAGTTCATGCTGATCCGGTTCGTTCTTTACCTG	GAAGGTCGGAGTCAACGGATTTGGATCCGGTTCGTTCTTTACCTA	TGTTGCCACAGCTGCTCCCTAC
SNP85	03	80315283	A，G	GAAGGTGACCAAGTTCATGCTTTTGTTGGGACTTTTCTGATTGCAGA	GAAGGTCGGAGTCAACGGATTTGTTGGGACTTTTCTGATTGCAGG	GGTAAAGTTTTCTTTGTTTTATACACTATCCC
SNP86	03	85343412	A，G	GAAGGTGACCAAGTTCATGCTATATAGCATATACATACACTACTGTTGCA	GAAGGTCGGAGTCAACGGATTTAGCATATACATACACTACTGTTGCG	TGTCTATGGGGTTTCTTAGAAGCAAAAG
SNP89	03	95236466	G，A	GAAGGTGACCAAGTTCATGCTTGAAATCCTACAAATTGGGCCTGG	GAAGGTCGGAGTCAACGGATTGTGAAATCCTACAAATTGGGCCTGA	ACTCTTTCCAGATCCAACTTGTAAGATTTTA
SNP91	04	4597507	T，C	GAAGGTGACCAAGTTCATGCTTTTATAAATATTTTAGGTTCGATTGCAAATCT	GAAGGTCGGAGTCAACGGATTTTTATAAATATTTTAGGTTCGATTGCAAATCC	CAAATTTGATCAAACCTAAAATATTTCTACTTCGAT
SNP117	06	22231775	G，T	GAAGGTGACCAAGTTCATGCTCTTCGGTCTAGATCTCTACCATTG	GAAGGTCGGAGTCAACGGATTCCTTCGGTCTAGATCTCTACCATTT	ACTCTTCAACTAATATTGTTGGGTTCATTGA
SNP121	06	49357773	T，C	GAAGGTGACCAAGTTCATGCTGGGCAAGTTAAGCCCAAGATATGT	GAAGGTCGGAGTCAACGGATTGGCAAGTTAAGCCCAAGATATGC	CTTGCCAATTCTCCTTATTACCCTATAAG
SNP131	06	80004443	C，G	GAAGGTGACCAAGTTCATGCTCCATGATCAATTTGCTGGAAATTTGC	GAAGGTCGGAGTCAACGGATTCCATGATCAATTTGCTGGAAATTTGG	GTAGCGGGGTGTCGTAGAGTTG
SNP134	06	90626817	G，A	GAAGGTGACCAAGTTCATGCTATTTATAGCAATTGTACTATGCATTAAAAGTG	GAAGGTCGGAGTCAACGGATTATTTATAGCAATTGTACTATGCATTAAAAGTA	TGTTTTTAACACATCATGCAATCTTTTTACTCAA
SNP137	07	6063306	T，C	GAAGGTGACCAAGTTCATGCTGGCTTGGTGAACATAGATTACCGTT	GAAGGTCGGAGTCAACGGATTGCTTGGTGAACATAGATTACCGTC	GATAACCTAATTCTTTTCTAGTGTATAGCATG
SNP138	07	6628015	C，A	GAAGGTGACCAAGTTCATGCTGACTTAACTCAAGACTCAACTCTTCA	GAAGGTCGGAGTCAACGGATTACTTAACTCAAGACTCAACTCTTCG	CTTAAAAACATGCATGATTTCTTGAGTTGAG
SNP141	07	18104673	G，A	GAAGGTGACCAAGTTCATGCTGAAGATCTGCTCATATTCCATACCG	GAAGGTCGGAGTCAACGGATTTGAAGATCTGCTCATATTCCATACCA	GCTGTATCCGTGAGTTATCTTATTTTGC
SNP142	07	22168025	G，A	GAAGGTGACCAAGTTCATGCTAAGCATTTTTGAGAGCATTAACCCG	GAAGGTCGGAGTCAACGGATTCAAGCATTTTTGAGAGCATTAACCCA	CTTCAAGTGCTAATGTGGATCAATGATATC
SNP143	07	28318066	T，C	GAAGGTGACCAAGTTCATGCTCATAACTAGTGGCGTAAGCCTAAAT	GAAGGTCGGAGTCAACGGATTCATAACTAGTGGCGTAAGCCTAAAC	ACACAATATCAACTAGGCACTACTATAATAG
SNP145	07	35925740	A，G	GAAGGTGACCAAGTTCATGCTTCCTACCTAGCAAACCCAACAGA	GAAGGTCGGAGTCAACGGATTCCTACCTAGCAAACCCAACAGG	GCGACCTTCTTAAGGAGGTACATATG
SNP146	07	45866335	T，C	GAAGGTGACCAAGTTCATGCTTATAGAGCTCTAGACCTCAACGATTT	GAAGGTCGGAGTCAACGGATTTAGAGCTCTAGACCTCAACGATTC	AGATTCTGTAGACAGGGGACTTCAG
SNP147	07	45866604	T，C	GAAGGTGACCAAGTTCATGCTTAAGATGGATTGAGTTGATAGCCCAT	GAAGGTCGGAGTCAACGGATTAGATGGATTGAGTTGATAGCCCAC	AGCGGAAGACGATGAAGAAATAACTAAC
SNP156	07	69502586	T，A	GAAGGTGACCAAGTTCATGCTACACGGATTTTGGAATATATGGAATTTAAAT	GAAGGTCGGAGTCAACGGATTACACGGATTTTGGAATATATGGAATTTAAAA	AGTCTCGAAAATGTGCCTCGGAATG
SNP160	07	79910819	T，G	GAAGGTGACCAAGTTCATGCTCATTTTCAATTCCACAAATGGATGATAGT	GAAGGTCGGAGTCAACGGATTATTTTCAATTCCACAAATGGATGATAGG	GATTCTTAATGTGATACAACATTATTAGCCCTT
SNP162	07	80408110	T，A	GAAGGTGACCAAGTTCATGCTTTCATGAACTCATCCACCTTTACCT	GAAGGTCGGAGTCAACGGATTTTCATGAACTCATCCACCTTTACCA	TTCTTGACCATTTCTTTCCTCAAGAGCT
SNP164	07	80408439	C，T	GAAGGTGACCAAGTTCATGCTATAGGAGCTTGAGGACCTCGC	GAAGGTCGGAGTCAACGGATTAAATAGGAGCTTGAGGACCTCGT	ACTCAGTTGGTGGCCTCCCAAG
SNP165	07	80469941	T，C	GAAGGTGACCAAGTTCATGCTGCACCCTCAGATCATCCACATTT	GAAGGTCGGAGTCAACGGATTCACCCTCAGATCATCCACATTC	TTGTGGACTTTGAAGAGGATTTGCATCA
SNP166	07	82108833	T，C	GAAGGTGACCAAGTTCATGCTATGGGTTGGGTAATTTCGCTCCT	GAAGGTCGGAGTCAACGGATTGGGTTGGGTAATTTCGCTCCC	ACATGGCAATGTCATAACATATTCATCTAGA
SNP171	07	92136286	T，G	GAAGGTGACCAAGTTCATGCTCATGCTTCAAGTGTGTGAAGCTCAT	GAAGGTCGGAGTCAACGGATTATGCTTCAAGTGTGTGAAGCTCAG	GCATCTCTACTCCTAAAATCTGTTACATG
SNP172	07	93000441	C，T	GAAGGTGACCAAGTTCATGCTACTTCTAGTGGTGAGAGGGAAC	GAAGGTCGGAGTCAACGGATTAGACTTCTAGTGGTGAGAGGGAAT	CCATATTATGGGCCCTTTTAGCCTC
SNP186	07	112287893	A，G	GAAGGTGACCAAGTTCATGCTATTTTTAGTATGAGGGATTTTCTTGCCTA	GAAGGTCGGAGTCAACGGATTTTTAGTATGAGGGATTTTCTTGCCTG	TGCCAGTCCAATAGAAAATAAATATCCTTAG
SNP189	07	122903454	T，G	GAAGGTGACCAAGTTCATGCTTCCTTCCTTTTCCTCAACATGTGTAT	GAAGGTCGGAGTCAACGGATTCCTTCCTTTTCCTCAACATGTGTAG	AGGAAAAGCCGAATACAGTTTGCTTAGT
SNP191	07	124417180	G，A	GAAGGTGACCAAGTTCATGCTCTTTGCTCTTTAATTAGCCTTTAATCAAG	GAAGGTCGGAGTCAACGGATTTCTTTGCTCTTTAATTAGCCTTTAATCAAA	GATGGAGTTTCTATTATAGCATCTTTTAATTTGAAA
SNP201	08	70256429	C，T	GAAGGTGACCAAGTTCATGCTCGCTTCGACGTTTTAGGCTTTC	GAAGGTCGGAGTCAACGGATTGCGCTTCGACGTTTTAGGCTTTT	CTTCAAGATTCCAATTGTATCATTTAAACGC
SNP217	09	16666015	T，C	GAAGGTGACCAAGTTCATGCTATAATGTGATTGGTCAAGGAAAATTCTATT	GAAGGTCGGAGTCAACGGATTTAATGTGATTGGTCAAGGAAAATTCTATC	GGCAAATTTTATTCCTAGAATAAAAATAACTAGAAC
SNP218	09	16666273	T，C	GAAGGTGACCAAGTTCATGCTCCTATATGGCTTGAAACAGGCACT	GAAGGTCGGAGTCAACGGATTCTATATGGCTTGAAACAGGCACC	GCAGGAATCAAACTTTTTCGTGTCATTG
SNP221	09	19600707	C，T	GAAGGTGACCAAGTTCATGCTTACCATACTTACTACAAGTAGGATAAC	GAAGGTCGGAGTCAACGGATTCTACCATACTTACTACAAGTAGGATAAT	CAGGGTAGCATCGCTCAAGGATC
SNP248	12	5901448	G，A	GAAGGTGACCAAGTTCATGCTGAGTGAAAAACTTTGTCTCTTTGCTG	GAAGGTCGGAGTCAACGGATTATTGAGTGAAAAACTTTGTCTCTTTGCTA	CAGTGGCACAATAGTGGGAGTGG
SNP252	12	12105247	C，A	GAAGGTGACCAAGTTCATGCTCAGTGGTAGAGGTTATTTGTCAGC	GAAGGTCGGAGTCAACGGATTTACAGTGGTAGAGGTTATTTGTCAGA	GTGACTAAGAAGGGGACAGCCAC
SNP257	12	48709022	G，A	GAAGGTGACCAAGTTCATGCTTAAATGGTAGCATTACAATTTCATGCATG	GAAGGTCGGAGTCAACGGATTTTATAAATGGTAGCATTACAATTTCATGCATA	GGTTAGTACAGTAGAATGTACTAAGTATATAAG
SNP259	12	53534315	C，T	GAAGGTGACCAAGTTCATGCTCATGAGTCATATGAAGTGTCTACTC	GAAGGTCGGAGTCAACGGATTGCATGAGTCATATGAAGTGTCTACTT	CTGTAAAATTTCCTAGGAACAATATATGCAC

ID	染色体 Chr	位置 Position	参考碱基，变异 Ref，Alt	X引物序列 Sequence of primer X	Y引物序列 Sequence of primer Y	Z引物序列 Sequence of primer Z
SNP 06	01	44620003	A，G	GAAGGTGACCAAGTTCATGCTTACACATGATCTTAAATTAGTGGCCA	GAAGGTCGGAGTCAACGGATTTACACATGATCTTAAATTAGTGGCCG	CAGATAGTGACACCATATTTTTAATGCAAATATG
SNP 07	01	53995173	T，A	GAAGGTGACCAAGTTCATGCTCTAGCCACGTGGAGGAGGATAT	GAAGGTCGGAGTCAACGGATTCTAGCCACGTGGAGGAGGATAA	CTATAATCATGTAGGGACAGGCGATC
SNP09	01	59104419	T，C	GAAGGTGACCAAGTTCATGCTCTGAGGACCTAACAACATTGTGCTT	GAAGGTCGGAGTCAACGGATTTGAGGACCTAACAACATTGTGCTC	GGCCTTAGTTTGGGTCTTGACAAACT
SNP24	01	83357891	A，C	GAAGGTGACCAAGTTCATGCTGTTCACTGTATTATAGCATGTTTGACA	GAAGGTCGGAGTCAACGGATTGTTCACTGTATTATAGCATGTTTGACC	GCGTATGTCTCAATAATCAATGTAAAATCAG
SNP25	01	83358171	C，T	GAAGGTGACCAAGTTCATGCTCGAGTCCTACATGTCGATCCTC	GAAGGTCGGAGTCAACGGATTTCGAGTCCTACATGTCGATCCTT	TTGGGCATGGCCATCGAAGCTC
SNP26	01	84072334	G，C	GAAGGTGACCAAGTTCATGCTTATGCCTACATGATGTTACCATGTAG	GAAGGTCGGAGTCAACGGATTTATGCCTACATGATGTTACCATGTAC	TCGGTTCCTACATGGTAACAGAGCT
SNP44	01	122998346	T，C	GAAGGTGACCAAGTTCATGCTTATTCCACCTCATTTTGAAACTCAGAAT	GAAGGTCGGAGTCAACGGATTTTCCACCTCATTTTGAAACTCAGAAC	TTGAGGAAGGAACCTAAGATTCATTTGC
SNP48	02	9305544	G，T	GAAGGTGACCAAGTTCATGCTACGGTCTAAACTAAGGGCCTAG	GAAGGTCGGAGTCAACGGATTTCACGGTCTAAACTAAGGGCCTAT	GGGTTCCCCTTGCGCTGTGTCA
SNP56	02	74119173	A，G	GAAGGTGACCAAGTTCATGCTAAATTCTCTTGCCAGACTTATTGAAAATATTA	GAAGGTCGGAGTCAACGGATTTTCTCTTGCCAGACTTATTGAAAATATTG	TGTTTGTATGTGGATGATTTGATCTTGATAG
SNP59	02	82393242	A，G	GAAGGTGACCAAGTTCATGCTTATAACCAAAGTGAAACATCAAAACTTCAA	GAAGGTCGGAGTCAACGGATTATAACCAAAGTGAAACATCAAAACTTCAG	TAATACTGGATTGTGTAAGTCTTCAACTTTG
SNP61	02	83239126	A，G	GAAGGTGACCAAGTTCATGCTGGTAAGCATTAATCAGGCCTGTCA	GAAGGTCGGAGTCAACGGATTGTAAGCATTAATCAGGCCTGTCG	GAAGTTTTAGTCCTCTACATACATAAAACTTAG
SNP72	03	25452216	C，T	GAAGGTGACCAAGTTCATGCTTCTTGTTTCTCCTTTACCTCCACC	GAAGGTCGGAGTCAACGGATTATCTTGTTTCTCCTTTACCTCCACT	TCCTTGTGCATAATGGATCTACATCATC
SNP84	03	76800537	G，A	GAAGGTGACCAAGTTCATGCTGATCCGGTTCGTTCTTTACCTG	GAAGGTCGGAGTCAACGGATTTGGATCCGGTTCGTTCTTTACCTA	TGTTGCCACAGCTGCTCCCTAC
SNP85	03	80315283	A，G	GAAGGTGACCAAGTTCATGCTTTTGTTGGGACTTTTCTGATTGCAGA	GAAGGTCGGAGTCAACGGATTTGTTGGGACTTTTCTGATTGCAGG	GGTAAAGTTTTCTTTGTTTTATACACTATCCC
SNP86	03	85343412	A，G	GAAGGTGACCAAGTTCATGCTATATAGCATATACATACACTACTGTTGCA	GAAGGTCGGAGTCAACGGATTTAGCATATACATACACTACTGTTGCG	TGTCTATGGGGTTTCTTAGAAGCAAAAG
SNP89	03	95236466	G，A	GAAGGTGACCAAGTTCATGCTTGAAATCCTACAAATTGGGCCTGG	GAAGGTCGGAGTCAACGGATTGTGAAATCCTACAAATTGGGCCTGA	ACTCTTTCCAGATCCAACTTGTAAGATTTTA
SNP91	04	4597507	T，C	GAAGGTGACCAAGTTCATGCTTTTATAAATATTTTAGGTTCGATTGCAAATCT	GAAGGTCGGAGTCAACGGATTTTTATAAATATTTTAGGTTCGATTGCAAATCC	CAAATTTGATCAAACCTAAAATATTTCTACTTCGAT
SNP117	06	22231775	G，T	GAAGGTGACCAAGTTCATGCTCTTCGGTCTAGATCTCTACCATTG	GAAGGTCGGAGTCAACGGATTCCTTCGGTCTAGATCTCTACCATTT	ACTCTTCAACTAATATTGTTGGGTTCATTGA
SNP121	06	49357773	T，C	GAAGGTGACCAAGTTCATGCTGGGCAAGTTAAGCCCAAGATATGT	GAAGGTCGGAGTCAACGGATTGGCAAGTTAAGCCCAAGATATGC	CTTGCCAATTCTCCTTATTACCCTATAAG
SNP131	06	80004443	C，G	GAAGGTGACCAAGTTCATGCTCCATGATCAATTTGCTGGAAATTTGC	GAAGGTCGGAGTCAACGGATTCCATGATCAATTTGCTGGAAATTTGG	GTAGCGGGGTGTCGTAGAGTTG
SNP134	06	90626817	G，A	GAAGGTGACCAAGTTCATGCTATTTATAGCAATTGTACTATGCATTAAAAGTG	GAAGGTCGGAGTCAACGGATTATTTATAGCAATTGTACTATGCATTAAAAGTA	TGTTTTTAACACATCATGCAATCTTTTTACTCAA
SNP137	07	6063306	T，C	GAAGGTGACCAAGTTCATGCTGGCTTGGTGAACATAGATTACCGTT	GAAGGTCGGAGTCAACGGATTGCTTGGTGAACATAGATTACCGTC	GATAACCTAATTCTTTTCTAGTGTATAGCATG
SNP138	07	6628015	C，A	GAAGGTGACCAAGTTCATGCTGACTTAACTCAAGACTCAACTCTTCA	GAAGGTCGGAGTCAACGGATTACTTAACTCAAGACTCAACTCTTCG	CTTAAAAACATGCATGATTTCTTGAGTTGAG
SNP141	07	18104673	G，A	GAAGGTGACCAAGTTCATGCTGAAGATCTGCTCATATTCCATACCG	GAAGGTCGGAGTCAACGGATTTGAAGATCTGCTCATATTCCATACCA	GCTGTATCCGTGAGTTATCTTATTTTGC
SNP142	07	22168025	G，A	GAAGGTGACCAAGTTCATGCTAAGCATTTTTGAGAGCATTAACCCG	GAAGGTCGGAGTCAACGGATTCAAGCATTTTTGAGAGCATTAACCCA	CTTCAAGTGCTAATGTGGATCAATGATATC
SNP143	07	28318066	T，C	GAAGGTGACCAAGTTCATGCTCATAACTAGTGGCGTAAGCCTAAAT	GAAGGTCGGAGTCAACGGATTCATAACTAGTGGCGTAAGCCTAAAC	ACACAATATCAACTAGGCACTACTATAATAG
SNP145	07	35925740	A，G	GAAGGTGACCAAGTTCATGCTTCCTACCTAGCAAACCCAACAGA	GAAGGTCGGAGTCAACGGATTCCTACCTAGCAAACCCAACAGG	GCGACCTTCTTAAGGAGGTACATATG
SNP146	07	45866335	T，C	GAAGGTGACCAAGTTCATGCTTATAGAGCTCTAGACCTCAACGATTT	GAAGGTCGGAGTCAACGGATTTAGAGCTCTAGACCTCAACGATTC	AGATTCTGTAGACAGGGGACTTCAG
SNP147	07	45866604	T，C	GAAGGTGACCAAGTTCATGCTTAAGATGGATTGAGTTGATAGCCCAT	GAAGGTCGGAGTCAACGGATTAGATGGATTGAGTTGATAGCCCAC	AGCGGAAGACGATGAAGAAATAACTAAC
SNP156	07	69502586	T，A	GAAGGTGACCAAGTTCATGCTACACGGATTTTGGAATATATGGAATTTAAAT	GAAGGTCGGAGTCAACGGATTACACGGATTTTGGAATATATGGAATTTAAAA	AGTCTCGAAAATGTGCCTCGGAATG
SNP160	07	79910819	T，G	GAAGGTGACCAAGTTCATGCTCATTTTCAATTCCACAAATGGATGATAGT	GAAGGTCGGAGTCAACGGATTATTTTCAATTCCACAAATGGATGATAGG	GATTCTTAATGTGATACAACATTATTAGCCCTT
SNP162	07	80408110	T，A	GAAGGTGACCAAGTTCATGCTTTCATGAACTCATCCACCTTTACCT	GAAGGTCGGAGTCAACGGATTTTCATGAACTCATCCACCTTTACCA	TTCTTGACCATTTCTTTCCTCAAGAGCT
SNP164	07	80408439	C，T	GAAGGTGACCAAGTTCATGCTATAGGAGCTTGAGGACCTCGC	GAAGGTCGGAGTCAACGGATTAAATAGGAGCTTGAGGACCTCGT	ACTCAGTTGGTGGCCTCCCAAG
SNP165	07	80469941	T，C	GAAGGTGACCAAGTTCATGCTGCACCCTCAGATCATCCACATTT	GAAGGTCGGAGTCAACGGATTCACCCTCAGATCATCCACATTC	TTGTGGACTTTGAAGAGGATTTGCATCA
SNP166	07	82108833	T，C	GAAGGTGACCAAGTTCATGCTATGGGTTGGGTAATTTCGCTCCT	GAAGGTCGGAGTCAACGGATTGGGTTGGGTAATTTCGCTCCC	ACATGGCAATGTCATAACATATTCATCTAGA
SNP171	07	92136286	T，G	GAAGGTGACCAAGTTCATGCTCATGCTTCAAGTGTGTGAAGCTCAT	GAAGGTCGGAGTCAACGGATTATGCTTCAAGTGTGTGAAGCTCAG	GCATCTCTACTCCTAAAATCTGTTACATG
SNP172	07	93000441	C，T	GAAGGTGACCAAGTTCATGCTACTTCTAGTGGTGAGAGGGAAC	GAAGGTCGGAGTCAACGGATTAGACTTCTAGTGGTGAGAGGGAAT	CCATATTATGGGCCCTTTTAGCCTC
SNP186	07	112287893	A，G	GAAGGTGACCAAGTTCATGCTATTTTTAGTATGAGGGATTTTCTTGCCTA	GAAGGTCGGAGTCAACGGATTTTTAGTATGAGGGATTTTCTTGCCTG	TGCCAGTCCAATAGAAAATAAATATCCTTAG
SNP189	07	122903454	T，G	GAAGGTGACCAAGTTCATGCTTCCTTCCTTTTCCTCAACATGTGTAT	GAAGGTCGGAGTCAACGGATTCCTTCCTTTTCCTCAACATGTGTAG	AGGAAAAGCCGAATACAGTTTGCTTAGT
SNP191	07	124417180	G，A	GAAGGTGACCAAGTTCATGCTCTTTGCTCTTTAATTAGCCTTTAATCAAG	GAAGGTCGGAGTCAACGGATTTCTTTGCTCTTTAATTAGCCTTTAATCAAA	GATGGAGTTTCTATTATAGCATCTTTTAATTTGAAA
SNP201	08	70256429	C，T	GAAGGTGACCAAGTTCATGCTCGCTTCGACGTTTTAGGCTTTC	GAAGGTCGGAGTCAACGGATTGCGCTTCGACGTTTTAGGCTTTT	CTTCAAGATTCCAATTGTATCATTTAAACGC
SNP217	09	16666015	T，C	GAAGGTGACCAAGTTCATGCTATAATGTGATTGGTCAAGGAAAATTCTATT	GAAGGTCGGAGTCAACGGATTTAATGTGATTGGTCAAGGAAAATTCTATC	GGCAAATTTTATTCCTAGAATAAAAATAACTAGAAC
SNP218	09	16666273	T，C	GAAGGTGACCAAGTTCATGCTCCTATATGGCTTGAAACAGGCACT	GAAGGTCGGAGTCAACGGATTCTATATGGCTTGAAACAGGCACC	GCAGGAATCAAACTTTTTCGTGTCATTG
SNP221	09	19600707	C，T	GAAGGTGACCAAGTTCATGCTTACCATACTTACTACAAGTAGGATAAC	GAAGGTCGGAGTCAACGGATTCTACCATACTTACTACAAGTAGGATAAT	CAGGGTAGCATCGCTCAAGGATC
SNP248	12	5901448	G，A	GAAGGTGACCAAGTTCATGCTGAGTGAAAAACTTTGTCTCTTTGCTG	GAAGGTCGGAGTCAACGGATTATTGAGTGAAAAACTTTGTCTCTTTGCTA	CAGTGGCACAATAGTGGGAGTGG
SNP252	12	12105247	C，A	GAAGGTGACCAAGTTCATGCTCAGTGGTAGAGGTTATTTGTCAGC	GAAGGTCGGAGTCAACGGATTTACAGTGGTAGAGGTTATTTGTCAGA	GTGACTAAGAAGGGGACAGCCAC
SNP257	12	48709022	G，A	GAAGGTGACCAAGTTCATGCTTAAATGGTAGCATTACAATTTCATGCATG	GAAGGTCGGAGTCAACGGATTTTATAAATGGTAGCATTACAATTTCATGCATA	GGTTAGTACAGTAGAATGTACTAAGTATATAAG
SNP259	12	53534315	C，T	GAAGGTGACCAAGTTCATGCTCATGAGTCATATGAAGTGTCTACTC	GAAGGTCGGAGTCAACGGATTGCATGAGTCATATGAAGTGTCTACTT	CTGTAAAATTTCCTAGGAACAATATATGCAC