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园艺学报 ›› 2023, Vol. 50 ›› Issue (12): 2568-2576.doi: 10.16420/j.issn.0513-353x.2022-0931

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

酸枣种质资源果实数量性状遗传多样性分析

段开行, 王晓玲, 毛永民, 王瑶, 任勇响, 任柳柳, 申连英*()   

  1. 河北农业大学园艺学院,河北保定 071000
  • 收稿日期:2023-05-04 修回日期:2023-08-28 出版日期:2023-12-25 发布日期:2023-12-29
  • 通讯作者:
    *(E-mail:
  • 基金资助:
    河北省重点研发计划项目(21326312D); 国家重点研发计划项目(2017YFC1701700); 国家林业和草原种质资源库项目(2005DKA21003)

Analysis of Genetic Diversity of Wild Jujube Germplasm Resources Based on Quantitative Characters

DUAN Kaihang, WANG Xiaoling, MAO Yongmin, WANG Yao, REN Yongxiang, REN Liuliu, SHEN Lianying*()   

  1. College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China
  • Received:2023-05-04 Revised:2023-08-28 Published:2023-12-25 Online:2023-12-29

摘要:

以河北、辽宁、陕西和山东等地393个酸枣种质资源为试材,对果实单果质量、单核质量、千仁质量、含糖量、含酸量等21个数量性状的遗传变异规律及多样性进行研究。结果表明,酸枣果实性状具有丰富的遗传多样性,其中18个数量性状变异明显,Shannon's指数大于1.0。不同地区间种质资源遗传多样性差异明显,多样性指数由高到低依次为:邢台(1.198)、邯郸(1.147)、朝阳(1.120)、保定(1.069)、榆林(0.953)和泰安(0.733)。通过聚类分析将所有酸枣种质划分为3大组,即华北组、陕西组和辽宁组,以邢台为代表的华北组酸枣种质多样性水平最高。从主成分分析的结果看,前4个主成分对变异的累计贡献率达71.85%,第一主成分主要反应了单果质量和千仁质量,第二主成分反应了可滴定酸含量,第三和第四主成分分别反应了核横径和维生素C含量。

关键词: 酸枣, 种质资源, 遗传多样性, 聚类分析, 主成分分析

Abstract:

Twenty-one quantitative characters(weight of single fruit,stone weight,a thouthand kernel weight,total sugar content,titratable acid content,etc.)of Ziziphus jujuba Mill. var. spinosa(Bunge)Hu ex H. F. Chow fruits'genetic variation rule and genetic diversity were studied in 393 wild jujube germplasm from follow provinces of Hebei,Liaoning,Shandong,Shaanxi.The results showed that the wild jujube germplasm was rich in genetic diversity. The obvious variety was observed with18 quantitative characters. The Shannon's index of the 18 quantitative characters exceeded 1.0. The degree of genetic diversity between various regions was distinctly different,and the upper-to-lower order of the various regions based on Shannon's index was Xingtai(H' = 1.198),Handan(H' = 1.147),Chaoyang(H' = 1.120),Baoding(H' = 1.069),Yulin(H' = 0.953),and Tai'an(H' = 0.733). Cluster analysis divided the wild jujube germplasm into three groups,North China,Shaanxi,Liaoning. The wild jujube germplasm in North China(mainly represented by Xingtai)was the richest in quantitative diversity,which provides a wealth of material to choose for the breeding. The principal component analysis on quantitative characters result showed that the accumulative contribution rate of the first four principal components accounted for 71.85% in the total variation accounted,and the first principal component mainly reflected the fruit mass and a thouthand kernel weight,while the second principal component reflected the titratable acid content. The third and fourth principal component reflected stone vertical diameter and vitamin C content,respectively.

Key words: Ziziphus jujuba, germplasm, genetic diversity, cluster analysis, principal component analysis