芥蓝—菜薹种间三倍体回交子代染色体数鉴定及单体异附加系的选育

园艺学报 ›› 2010, Vol. 37 ›› Issue (2): 213-220.

芥蓝—菜薹种间三倍体回交子代染色体数鉴定及单体异附加系的选育

任艳蕊;张成合*;申二巧;轩淑欣;申书兴

（河北农业大学园艺学院，河北保定071001）

收稿日期:2009-12-22 修回日期:2010-01-25 出版日期:2010-02-25 发布日期:2010-02-25
通讯作者: 张成合

Chromosome Number Identification and Generation of Monosomic AlienAddition Line in the Triploid Interspecies Hybrid of Flowering ChineseCabbage–Chinese Kale

REN Yan-rui;ZHANG Cheng-he*;SHEN Er-qiao;XUAN Shu-xin; SHEN Shu-xing

(College of Horticulture，Agricultural University of Hebei，Baoding，Hebei 071001，China)

Received:2009-12-22 Revised:2010-01-25 Online:2010-02-25 Published:2010-02-25
Contact: ZHANG Cheng-he

摘要/Abstract

摘要： 为创造CC 染色体组附加A 染色体组的单体异附加系，以芥蓝—菜薹种间三倍体（CCA）为材料，采用人工杂交、形态和细胞学鉴定及核型分析等方法，比较了芥蓝—菜薹种间三倍体与其亲本芥蓝（CC）正、反交的结实性，分析了杂交子代的染色体数及频率分布，并对筛选出的单体异附加系进行了系统研究。结果表明：CCA × CC 和CC × CCA 的结籽率分别为0.28%和0.63%；在鉴定的88 个CC × CCA 的子代中，全部为2n = 18 的整倍体植株；在鉴定的156 个CCA × CC 的子代中，(2n + 1) ~ (2n + 9)等各类非整倍体均有存在，其中附加1 条染色体的1 个、附加2 条染色体的16 个；形态学标记鉴定和核型分析显示附加1 条染色体的那个子代植株为单体异附加系CC + A9；在减数分裂终变期，单体异附加系CC + A9 的A9 染色体常与一对C 组的染色体发生局部联会，两者间存在着部分同源关系；单体异附加系CC + A9 的n + 1 雌、雄配子均有一定传递率，分别为18.75%和9.82%；单体异附加系CC + A9 自交获得了1 株双体异附加系。

关键词: 芥蓝, 菜薹, 异源三倍体, 异附加系

Abstract: In order to generate monosomic alien addition line of C-genome added the chromosome derived from A-genome, the triploid interspecies hybrid (CCA) and its parent Chinese kale (Brassica alboglabra Bailey) and Flowering Chinese cabbage (Brassica campestris L. var. utilis Tsen et Lee) were used as the basic materials, by means of artificial hybridization, morphological and cytological examination and karyotype analysis, the seed setting of CCA × CC and CC × CCA, the chromosome number of their hybrids and the monosomic alien addition line of CC genome added a A-chromosome were identified. The main results showed as follows：① The seed setting of CCA × CC and CC × CCA was 0.28% and 0.63% respectively；② All of 88 progeny plants identified in CC × CCA were euploid（2n = CC = 18）；③
Aneuploids with different number extra chromosomes were existed in the identified 156 progeny plants of CCA × CC, in which one 2n + 1 plant and sixteen 2n + 2 plants were obtained；④ The monosomic alien addition line CC + A9 was preliminary determined by morphological marker identification and karyotype analysis for the 2n + 1 plant; ⑤ Meiotic observation for the addition line CC + A9 showed A9 chromosome having partial homology with a pair of C-chromosomes; ⑥ The alien chromosome A9 of the monosomic
addition line CC + A9 could be transmitted by male and female gametes with a percentage of 9.82% and 18.75% , respectively.

Key words: flowering Chinese cabbage, Brassica campestris L. var. utilis Tsen et Lee, Chinese kale, Brassica alboglabra Bailey, allotriploid, alien addition line

任艳蕊;张成合;申二巧;轩淑欣;申书兴. 芥蓝—菜薹种间三倍体回交子代染色体数鉴定及单体异附加系的选育[J]. 园艺学报, 2010, 37(2): 213-220.

REN Yan-rui;ZHANG Cheng-he;SHEN Er-qiao;XUAN Shu-xin;SHEN Shu-xing. Chromosome Number Identification and Generation of Monosomic AlienAddition Line in the Triploid Interspecies Hybrid of Flowering ChineseCabbage–Chinese Kale[J]. ACTA HORTICULTURAE SINICA, 2010, 37(2): 213-220.

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