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园艺学报 ›› 2009, Vol. 36 ›› Issue (10): 1491-1497.

• 观赏植物 • 上一篇    下一篇

不同倍性蝴蝶兰杂交后代的染色体倍性研究

周建金; 曾瑞珍; 刘芳; 易懋升; 黎扬辉; 张志胜;   

  1. 1华南农业大学广东省植物分子育种重点实验室,广州510642; 2广州花卉研究中心,广州510360
  • 收稿日期:2009-02-27 修回日期:2009-07-27 出版日期:2009-10-25 发布日期:2009-10-25

Investigation on Chromosome Ploidy of the Hybrids of Phalaenops is Polyploids

ZHOU Jian-jin1, ZENG Rui-zhen1, LIU Fang1, YIMao-sheng2, LIYang-hui2, and ZHANG Zhi-sheng1*   

  1. 1GuangdongProvincialKeyLaboratory ofPlantMolecularBreeding, South China AgriculturalUniversity, Guangzhou 510642,
    China;2Guangzhou FlowerResearch Center, Guangzhou 510360, China
  • Received:2009-02-27 Revised:2009-07-27 Online:2009-10-25 Published:2009-10-25

摘要: 对不同倍性蝴蝶兰小孢子母细胞减数分裂及其杂交后代的根尖染色体数进行了检测。结果表
明,除三倍体和三倍体杂交未能产生杂交后代外,不同倍性蝴蝶兰杂交后代的倍性均出现分离。二倍体和
二倍体、二倍体和四倍体杂交均产生二倍体、三倍体和四倍体;二倍体和三倍体杂交产生二倍体、三倍体、
五倍体和非整倍体;三倍体和四倍体随正反交不同而异,三倍体@四倍体的后代倍性有三倍体、四倍体、
八倍体和非整倍体,而四倍体@三倍体的后代倍性有三倍体、四倍体、五倍体和非整倍体;四倍体和四倍
体杂交产生二倍体、三倍体、四倍体、五倍体、六倍体和非整倍体。不同倍性蝴蝶兰的小孢子母细胞减数
分裂普遍存在微核、落后染色体和三分体等异常现象,导致小孢子和杂种后代的倍性出现多样性。

关键词: 蝴蝶兰, 多倍体, 减数分裂, 小孢子

Abstract: The microspore mother cell meiosis of different ploidy Phalaenopsis and chromosome number in root tip cell of their hybridswere observed. The results showed that the p loidy segregation appeared in all combinations excep t that of triploid ×triploid, in which no hybrid plants were obtained. Diploid, triploid and tetraploid plants were identified in the combinations of diploid ×diploid, dipoid ×tetraploid and tetraploid ×diploid Phalaenopsis. Diploids, triploids, pentap loids and aneuploids were found in the combinations of diploid ×trip loid and tripoid ×diploid. Triploids, tetraploids, octoploids and aneuploids were identified in the plants of triploid ×tetraploid combinations, while triploids, tetraploids, pentap loids and aneup loids existed in the plants of tetraploid trip loid ones. This indicated that ploidy segregation was related to reciprocal cros
ses. Tetraploid crossed with tetraploid produced diploids, triploids, tetraploids, pentaploids, hexaploids and aneuploids. The ploidy diversity of microspore in Phalaenopsis caused by the abnormal meiosis of microspore mother cells such as chromosome lagging, micronucleus and triad, was one of the main reasons resulting in ploidy diversity of the hybrids.

Key words: Phalaenopsis, polyp loid, meiosis, microspore

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