园艺学报 ›› 2008, Vol. 35 ›› Issue (7): 1047-1052.

• 其它园艺植物 • 上一篇    下一篇


张海凤1;郭宝林1*;张成合2;杨俊霞1;郭 婧3;陈新华1   

  1. 1河北农业大学林学院,河北保定 071001;2河北农业大学园艺学院,河北保定071001;3河北农业大学农学院,河北保定 071001)
  • 收稿日期:2008-02-01 修回日期:2008-05-22 出版日期:2008-07-25 发布日期:2008-07-25
  • 通讯作者: 郭宝林

Induction and Identification of Tetraploids in Eucommia ulmoides Oliv.

ZHANG Hai-feng1, GUO Bao-lin1*, ZHANG Cheng-he2,YANG Jun-xia1,GUO Jing3 ,and CHEN Xin-hua1   

  1. 1College of Forestry, Agricultural University of Hebei, Baoding 071001, China;2 College of Horticulture, Agricultural University of Hebei, Baoding 071001, China;3College of Agronomy, Agricultural University of Hebei, Baoding 071001, China)
  • Received:2008-02-01 Revised:2008-05-22 Online:2008-07-25 Published:2008-07-25
  • Contact: GUO Bao-lin

摘要: 以杜仲籽苗为材料,采用秋水仙素药液处理生长点的诱变方法及茎尖细胞染色体数目检测和DNA相对含量测定的倍性鉴定方法,研究了秋水仙素药液浓度,处理时间的诱变效果。结果表明,诱变率因处理的药液浓度和处理时间而异,除0.05%处理48 h外,其它浓度(0.1%,0.2%,0.4%)均以处理12 h效果最好,变异株率在43.3%~63.3%, 最佳处理组合为0.1%秋水仙素处理生长点12 h, 变异株率和四倍体株率分别为63.3%和36.7%。在获得的148个变异株中,染色体计数表明47株植株为四倍体(2n=4x=68),占31.76%;染色体计数结果进一步被流式细胞仪分析验证。四倍体植株表现出多倍体特征,叶片变厚,叶面积增大,叶形指数减小,保卫细胞增大,单位面积气孔数减少,叶绿体数明显增多。

关键词: 杜仲, 四倍体诱导, 倍性鉴定, 秋水仙素

Abstract: Stem primodia of Eucommia ulmoides Oliv. seedlings were treated with different concentrations of colchicine solution at different stages in order to figure out the best inducing method and obtain tetraploids of Eucommia ulmoides. The results showed that, apart from 0.05% colchicine solution for 48 h, the additional treatments (0.1%, 0.2% or 0.4%) were suitable for 12 h, in which the rates of mutants with morphological characteristics varied from 43.3% to 63.3%, the optimum treatment combination was 0.1% colchicine solution for 12 h, and the rates of vatiant plants and tetraploid plants were 63.3% and 36.7%, respectively. The results of chromosome counts indicated that, of the 148 variant plants, 47 plants were tetraploid plants (2n=4x=68), taking up about31.76%. This result was consistent with that analyzed with the 'Flow Cytometry' method. The plants showed the general characteristics of tetraploids, such as, thicker leaf, smaller leaf index , larger guard cells, less stomata per unit area and more chloroplasts in guard cells.

Key words: Eucommia ulmoides Oliv., polyploid induction, chromosome identification, colchicine