园艺学报 ›› 2008, Vol. 35 ›› Issue (2): 157-162.

• 果树 •    下一篇


高华君1,2;杨洪强1*;张 伟1   

  1. 1山东农业大学园艺科学与工程学院,山东泰安 271018;2作物生物学国家重点实验室,山东泰安 271018;3山东省果树研究所,山东泰安 271000)
  • 收稿日期:2007-07-17 修回日期:2007-11-26 出版日期:2008-02-25 发布日期:2008-02-25
  • 通讯作者: 杨洪强

Effects of Nitric Oxide on Lateral Root Formation Induced by IBA in Malus hupehensis Rehd. Seedlings

GAO Huajun1,2,YANG Hongqiang1,2* ,and ZHANG Wei1   

  1. (1College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China; 2State Key Laboratory of Crop Biology, Tai'an, Shandong 271018, China; 3Shandong Institute of Pomology, Tai'an, Shandong 271000, China)
  • Received:2007-07-17 Revised:2007-11-26 Online:2008-02-25 Published:2008-02-25
  • Contact: YANG Hongqiang

摘要: 本实验以平邑甜茶(Malus hupenhensis Rehd.)实生苗为材料,研究了一氧化氮(NO)对侧根的诱导效果、吲哚丁酸(IBA)对根系内源NO含量的影响以及NO在 IBA诱导侧根形成中的作用。结果表明,外源NO能够促进平邑甜茶侧根的形成与生长,并有明显的剂量效应,其中10 μmol/L NO供体硝普钠(SNP)处理后新根最长,50 μmol/L SNP处理后新根数量最多。100 μmol/L IBA处理后15~30 min和48~96 h,幼苗根系NO生成量出现两次显著升高,而IBA处理96 h后侧根原基数量大量增加。此外,蛋白激酶抑制剂Quercetin明显抑制IBA对NO的诱导。

关键词: 平邑甜茶, 侧根, 一氧化氮, 吲哚丁酸

Abstract: Nitric oxide (NO) is an important signaling molecule in plants and auxin is the main hormone regulating root development. Effects of exogenous NO on lateral root (LR) formation as well as indole butyric acid (IBA) on root endogenous NO production in Malus hupehensis Rehd. seedlings were studied. The results showed that application of the NO donor sodium nitroprusside (SNP) induced LR development in a dose-dependent manner. SNP concentrations of 10 μmol/L and 50 μmol/L were the most effective in promoting LR elongation and emergence, respectively. Two peaks of endogenous NO production in roots were observed from 15 min to 30 min and from 48 h to 96 h after 100 μmol/L IBA treatment. Coinciding with endogenous NO production, lateral root primordium (LRP) also increased greatly at 96 h after IBA application. Additionally, Quercetin (a protein kinase inhibitor) inhibited IBA-induced NO production in roots greatly. The roles of NO in IBA-induced LR production were discussed.

Key words: Malus hupehensis Rehd., lateral root, nitric oxide, IBA