同源四倍体青花菜离体再生及其倍性鉴定

园艺学报 ›› 2008, Vol. 35 ›› Issue (10): 1517-1520.

同源四倍体青花菜离体再生及其倍性鉴定

张丽丽,张蜀宁^*,张红梅,侯喜林

（南京农业大学作物遗传与种质创新国家重点试验室，南京210095）

收稿日期:2008-06-16 修回日期:2008-09-08 出版日期:2008-10-25 发布日期:2008-10-25
通讯作者: 张蜀宁

Regeneration of Autotetraploid Broccoli in Vitro Culture and Identification of the Ploidy

ZHANG Li-li;ZHANG Shu-ning^*;ZHANG Hong-mei;and HOU Xi-lin

（State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agriculture University, Nanjing 210095, China）

Received:2008-06-16 Revised:2008-09-08 Online:2008-10-25 Published:2008-10-25
Contact: ZHANG Shu-ning

摘要/Abstract

摘要： 以同源四倍体青花菜带柄子叶为外植体，研究其离体再生体系。结果表明： 10 d苗龄外植体在MS + 0.05 mg·L^-1 NAA + 3.0 mg·L^-1 6-BA+0.8% 琼脂 + 3% 蔗糖培养基上不定芽的再生频率最高为63.3％；继代培养(MS + 0.04 mg·L^-1 NAA + 4.0 mg·L^-1 6-BA + 0.8% 琼脂 + 3% 蔗糖)增殖系数为6，诱导(1/2MS + 0.1 mg·L^-1 NAA + 0.7% 琼脂 + 3% 蔗糖)生根率100％，移栽成活率90％。流式细胞技术分析及染色体计数鉴定结果表明，再生植株的DNA相对含量为二倍体的2 倍，染色体数为2n=4x=36。

关键词: 青花菜, 同源四倍体, 带柄子叶, 再生, 流式细胞仪

Abstract: The frequency of shoot regeneration in autotetraploid broccoli was studied. Cotyledons with petiole as explants were cultured on MS media with different combinations of growth regulator. The effects of growth regulator combinations on induction of shoots were evaluated. The optimal media for inducting shoots were MS media containing 0.8% agar and 3% sucrose supplemented with 3.0 mg·L^-1 6-BA, 0.05 mg·L^-1 NAA. The shoot induction percentage was 63.3%. Then each shoot was transplanted on the MS medium containing 0.04 mg·L^-1 NAA and 4.0 mg·L^-1 6-BA proliferating about 6 times. The use of 0.1 mg·L^-1 NAA in the rooting medium increased the number of healthy roots per rooted plant (root ratio was 100%). In this study, we established high-efficient shoot regeneration system of autotetraploid broccoli and analyzed the ploidy stability of regenerates using flow cytometry. The result showed that the tetraploid relative DNA content was about two times of control. The identified result of flow cytometry according to the chromosome analysis implied the regeneration plantlets keep the original ploidy.

Key words: broccoli, autotetraploid, cotyledons with petiole, in vitro culture, flow cytometry machine

中图分类号:

S 635

张丽丽;张蜀宁;张红梅;侯喜林. 同源四倍体青花菜离体再生及其倍性鉴定[J]. 园艺学报, 2008, 35(10): 1517-1520.

ZHANG Li-li;ZHANG Shu-ning;ZHANG Hong-mei;and HOU Xi-lin. Regeneration of Autotetraploid Broccoli in Vitro Culture and Identification of the Ploidy[J]. ACTA HORTICULTURAE SINICA, 2008, 35(10): 1517-1520.

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