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园艺学报 ›› 2008, Vol. 35 ›› Issue (1): 143-146.

• 研究简报 • 上一篇    下一篇

热激处理对蝴蝶兰组培褐变的影响

杨 玲1,2;葛 红2*;黄绵佳1;赵 滢2   

  1. (1华南热带农业大学 海南儋州 571737,2中国农业科学院蔬菜花卉研究所 北京 100081 )
  • 收稿日期:2007-05-29 修回日期:2007-08-18 出版日期:2008-01-25 发布日期:2008-01-25
  • 通讯作者: 葛 红

The Influence of Heat Shock on Tissue Browning of Phalaenopsis

YANG Ling1,2,GE Hong2*,HUANG Mian-jia1,and ZHAO Ying2
  

  1. (1South China University of Tropical Agriculture, Danzhou, Hainan 571737,China,2Institute of Vegetables and Flowers of Chinese Academy of Agricultural Sciences, Beijing 100081,China)
  • Received:2007-05-29 Revised:2007-08-18 Online:2008-01-25 Published:2008-01-25

摘要: 以蝴蝶兰叶片为试验材料,研究热激处理在抑制组织褐变中的作用。采用45 ℃条件下热激处理10 min,再经过常温下的不同恢复时间后,对叶片切割接种。然后在不同培养时间,对组织的褐化指数、总酚含量以及PAL等褐变相关指标进行测定比较。结果表明,采用热激处理的蝴蝶兰,再经常规培养条件下(25 ℃)培养48 h后,进行切割接种,可显著减轻随后培养过程中的褐变程度。

关键词: 蝴蝶兰, 组织培养, 褐变, 热激处理, 恢复时间, 酚类, 苯丙氨酸解氨酶

Abstract: To clearify the effect of heat shock on tissue browning of Phalaenopsis, the post-subculture changes in browning index, total phenolic content and PAL activity in tissues recovered under 25 ℃ for different time after heat shock treatment at 45 ℃ for 10 min were determined and analysed. The results suggested that the browning of cultured Phalaenopsis tissue could be significantly inhibited by heat treatment when the treated tissue was covered for 48 h under 25 ℃.

Key words: Phalaenopsis, tissue, browning, heat shock, restoration time, phenols, phenylalanine ammonia-lyase

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