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园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1471-1476.

• 蔬菜 • 上一篇    下一篇

植物内生放线菌防治西葫芦白粉病的初步研究

王美英;黄丽丽*;涂 璇;高小宁;阿里玛斯;姚 敏;康振生*   

  1. (西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,陕西杨凌 712100)
  • 收稿日期:2007-06-19 修回日期:2007-09-10 出版日期:2007-12-25 发布日期:2007-12-25
  • 通讯作者: 黄丽丽,康振生

Biocontrol of Zucchini Powdery Mildew by Endophytic Actinomycetes

WANG Mei-ying,HUANG Li-li*,TU Xuan,GAO Xiao-ning, ALMAS, YAO Min, and KANG Zhen-sheng*   

  1. (College of Plant Protection,Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University , Yangling, Shaanxi 712100, China)
  • Received:2007-06-19 Revised:2007-09-10 Online:2007-12-25 Published:2007-12-25

摘要: 以西葫芦白粉病为对象,通过温室盆栽试验和叶盘筛选试验,从50个内生放线菌菌株中筛选出2个防病效果较好的菌株,叶盘筛选试验发现菌株GKSHJA和PR1-8的无菌滤液原液在接种病菌的同时使用防治效果最佳,分别达到60.98%和63.22%。温室人工接种白粉病菌的盆栽试验发现,GKSHJA无菌滤液原液在接种前、接种后24 h喷雾,相对防治效果分别达55.22%和16.44%;菌株PR1-8的相对防效分别为51.94%和24.39%,且GKSHJA和PR1-8无菌滤液的5倍稀释液在接种病菌的同时使用也有一定的防病效果,其病情指数分别比对照降低了27.81%和30.19%。在自然感病的田间试验中,菌株GKSHJA和PR1-8无菌滤液的5倍稀释液对西葫芦白粉病的相对防效分别达到56.33%和69.88%。这些结果表明,菌株GKSHJA和PR1-8作为防治瓜类白粉病的生防菌株,具有开发应用潜力和前景。

关键词: 西葫芦, 白粉病, 内生放线菌, 病情指数, 防治效果

Abstract: Fifty strains of endophytic actinomycetes were screened in greenhouse pot experiments and leaf-disc test in lab. Two strains showed stable control effect on zucchini powdery mildew. In the leaf-disc test, the application of cell free filtrate of strains GKSHJA and PR1-8 showed 60.98% and 63.22% disease control efficiency, respectively. The control effect of strain GKSHJA with application 24 h before and after inoculation were 55.22% and 16.44%, respectively. The control effect of strain PR1-8 were 51.94% and 24.39% in the greenhouse potted seedlings inoculated with powdery mildew. The preinoculation application of cell free filtrate showed much higher disease control efficiency than postinoculation application. The applications of 5 times diluted cell free filtrates showed 27.81% (in GKSHJA treatment) and 30.19% (in PR1-8 treatment) less disease index than that in untreatment control. In field experiment, the control effect of biocontrol strains GKSHJA and PR1-8 were 56.33% and 69.88%, respectively, after application of 5 times dilution of cell free filtrate on naturally infected zucchini plants. These results showed that strains GKSHJA and PR1-8 were really potential biocontrol endophytic actinomycetes to control cucurbit powdery mildew and this research made a prior period research foundation for development and application of bio-pesticides on cucurbits powdery mildew.

Key words: Cucurbita pepo Linn, Sphaerotheca fuliginea, Endophytic antinomycetes, Control effect

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