甜樱桃与中国樱桃杂种的胚抢救及杂种鉴定

园艺学报 ›› 2004, Vol. 31 ›› Issue (3): 303-308.

甜樱桃与中国樱桃杂种的胚抢救及杂种鉴定

刘焕芳;陈学森^*;段成国;杨红花;冯宝春

(山东农业大学果树生物学实验室，泰安271018)

收稿日期:2003-11-28 修回日期:2004-03-31 出版日期:2004-06-25 发布日期:2004-06-25
通讯作者: 陈学森

Embryo Rescue and Identification of Hybrids between Sweet Cherry and Chinese Cherry

Liu Huanfang;Chen Xuesen;Duan Chengguo;Yang Honghua;Feng Baochun

(Biological Lab.of Pomology，Shandong Agricultural University，Tai’an 271018，China)

Received:2003-11-28 Revised:2004-03-31 Online:2004-06-25 Published:2004-06-25

摘要/Abstract

摘要： 以甜樱桃为母本，中国樱桃为父本，进行种间杂交，建立了杂种胚抢救技术体系，并对部分
杂种进行了鉴定。结果表明：(1)母本中，‘养老’坐果率最高，‘养老’在授粉4周后胚发生败育，而‘那翁’与‘红灯’则是在第3周后败育；(2)最佳萌发与生长培养基为1／2 MS+BA 2 mg／L+IAA 1 mg／L+维生素c 10 mg／L，最佳继代增殖培养基为1／2 MS+BA 2 mg／L+IAA 1 mg／L+NAA 0．1 mg／L+维生素c 10 mg／L，最佳生根培养基为1／2 MS+IBA 0．2 mg／L+维生素C lOmg／L；(3)S-allele specific PCR、RAPD及叶片形态鉴定结果证实了杂种的真实性.

关键词: 樱桃, 杂种, 胚抢救, 特异PCR, RAP

Abstract: Interspecific hybridization was carried out with selecting sweet cherry as female parent and Chinese cherry as male parent．Embryo rescue system of hybrids were studied and identification of them Was also made．The results showed that：(1)setting percentage was highest when ‘Ehon’was female parent；embryo abortion happened 4 weeks after pollination in ‘Ehon’，3 weeks in ‘Napoleon’and ‘Hongdeng’；(2)1／2 MS medium supplemented with BA 2 mg／L，IAA 1 mg／L and vitamin C 10 mg／L was the optimal medium for germination and growth，1／2 MS medium supplemented with BA 2 mg／L，IAA 1 mg／L，AA 0．1 mg／L and vitamin C 1 0 mg／L Was the optimal medium for multiplication，1／2 MS medium supplemented with IBA 0．2 mg／L and vitamin C 10 mg／L was the optimal medium for rootage；(3)S-allele specific PCR、RAPD and leaf shape analysis showed that they were real hybrids．

Key words: Cherry, Hybrids, Embryo rescue, S-allele specific PCR, RAPD

中图分类号:

s662.5

刘焕芳;陈学森;段成国;杨红花;冯宝春. 甜樱桃与中国樱桃杂种的胚抢救及杂种鉴定[J]. 园艺学报, 2004, 31(3): 303-308.

Liu Huanfang;Chen Xuesen;Duan Chengguo;Yang Honghua;Feng Baochun. Embryo Rescue and Identification of Hybrids between Sweet Cherry and Chinese Cherry[J]. ACTA HORTICULTURAE SINICA, 2004, 31(3): 303-308.

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