关键词: 山杜英, 离体培养, 植株再生, 封口材料

Abstract:

This paper mainly dealt with the study on shoot organogensis culture in vitro and plant regeneration from sprout explants of Elaeocarpus sylvestris (Lour．)Poir．The results showed that the best media for various stages were as follows：(1) Initial medium：MS+BA 1.0~2.0 mg·L^-1 +NAA 0.05 mg·L^-1 +3% sucrose；(2)Clump shoot regeneration medium from callus proliferation：MS+BA 1.0mg·L^-1 +NAA 0.5mg·L^-1 +3%sucrose；Clump shoot regeneration medium from axillary buds：MS+BA 2.0 mg·L^-1 +IBA 0.1 mg·L^-1 +3%sucrose；(3)Efficiency seedling induction medium：MS+BA 0.5
mg·L^-1 +IBA 0.1 mg·L^-1 +GA 1.0mg·L^-1 +3%sucrose；(4) Rooting medium：1/2 MS+IBA 3.0 mg·L^-1 +2%sucrose． In root induction culture．the rate of root formation with membrane filter is higher than that of root form ation with common membrane．

Key words: Elaeocarpus sylvestris (Lour．) Poir．, In vitro culture, Plant regeneration, Material of sealin