Abstract:

The strawberry fruits(Fragaria ananassa Duch．‘Chunxing’) harvested at white stage were used to investigate the efects of exogenous ethylene (50 µL·L^-1 ) on Ca²⁺-ATPase activities，O production rate and MDA content of microsomal membrane with or without pretreatment of 100 µmol·L^-1 chloropromaize(CPZ)，trifluoperizine(TFP)and verapamil(VER)，respectively，The results showed that the exogenous ethylene had no obvious influence on O production rate，and had no much effect on MDA content，but stimulated Ca²⁺-ATPase activitv of microsomal membrane at 12 h after ethylene treatment．Whereas a decreasing of Ca²⁺-ATPase activities， and relatively higher MDA content were found at 24 h after ethylene removal．The pretreatment of CPZ,TFP and VER reduced the Ca²⁺-ATPase activities and MDA content of microsomal memb rane under ethylene treatment， but had no significant efect on O production rate．These suggested that cellular Ca²⁺-CaM may be involved in regulation of Ca²⁺-ATPase activity and lipid peroxidation of microsomal membrane induced by ethylene．

Key words: Strawberry, Ethylene, Calcium messenger system, Fruit, Membrane, Ca²⁺-ATPase, Lipid peroxidation