圆柏组织培养体系的建立

doi:10.16420/j.issn.0513-353x.2025-0038

摘要/Abstract

摘要： 以圆柏（Sabina chinensis）鳞叶、针叶茎段为外植体，探讨不同消毒处理、培养基、抗褐变剂、植物生长调节剂配比对茎段增殖、生根的影响。结果表明：适宜鳞叶、针叶的0.1% HgCl₂消毒时间分别为18、16 min，污染率为8.52%、2.51%。培养基内分别添加1.0、2.0 mg · L^-1PVP抗鳞叶、针叶褐变。鳞叶最佳初代培养基是DCR + 2.0 mg · L^-16-BA + 0.5 mg · L^-1 NAA + 1.0 mg · L^-1PVP，芽诱导率为83.27%；针叶是DCR + 2.0 mg · L^-16-BA + 0.1 mg · L^-1 NAA + 2.0 mg · L^-1PVP，芽诱导率为89.35%。鳞叶最佳增殖培养基是DCR + 3.0 mg · L^-16-BA + 0.5 mg · L^-1Trans-ZT + 0.5 mg · L^-1NAA + 1.0 mg · L^-1PVP，增殖率为88.93%；针叶是DCR + 3.0 mg · L^-16-BA + 0.1 mg · L^-1Trans-ZT + 0.1 mg · L^-1NAA + 2.0 mg · L^-1PVP，增殖率为93.42%。鳞叶最佳生根培养基是1/2MS + 1.0 mg · L^-1NAA + 1.0 mg · L^-1IBA + 1.0 mg · L^-1PVP，生根率为73.29%；针叶是DCR + 2.0 mg · L^-1IBA + 2.0 mg · L^-1PVP，生根率达71.64%。鳞叶、针叶植株在草炭︰蛭石（体积比）为1︰1的基质中成活率分别为76%、60%。

关键词: 圆柏, 叶形, 褐变, 生根

Abstract:

Using stem segments of Sabina chinensis scale leaves and needle leaves as explants，the effects of different disinfection treatments，culture media，anti-browning agents，and plant hormone ratio on stem segment proliferation and rooting were investigated. The results showed that the optimal disinfection time of 0.1% HgCl₂ for scale leaves and needle leaves was 18 and 16 min，and the pollution rate was 8.52% and 2.51%，respectively. Adding 1.0 mg · L^-1 and 2.0 mg · L^-1PVP to the medium was suitable for anti-browning of scale leaves and needle leaves，respectively. The optimal primary culture medium for scale leaves was DCR + 2.0 mg · L^-1 6-BA + 0.5 mg · L^-1 NAA + 1.0 mg · L^-1PVP，with a bud induction rate of 83.27%，while for needle leaves was DCR + 2.0 mg · L^-1 6-BA + 0.1 mg · L^-1NAA + 2.0 mg · L^-1 PVP，with a bud induction rate of 89.35%. The optimal proliferation medium for scale leaves was DCR + 3.0 mg · L^-1 6-BA + 0.5 mg · L^-1 Trans-ZT + 0.5 mg · L^-1NAA + 1.0 mg · L^-1PVP，with a proliferation rate of 88.93%，while for needle leaves，it was DCR + 3.0 mg · L^-1 6-BA + 0.1 mg · L^-1 Trans-ZT + 0.1 mg · L^-1 NAA + 2.0 mg · L^-1PVP，with a proliferation rate of 93.42%. The optimal rooting medium for scale leaves was 1/2MS + 1.0 mg · L^-1 NAA + 1.0 mg · L^-1 IBA + 1.0 mg · L^-1 PVP，with a rooting rate of 73.29%，while for needle leaves，it was DCR + 2.0 mg · L^-1 IBA + 2.0 mg · L^-1 PVP，with a rooting rate of 71.64%. The survival rates of scale leaves and needle leaves plants in the substrate with a volume ratio of peat to vermiculite of 1︰1 was 76% and 60% respectively.

Key words: Sabina chinensis, leaf shape, browning, rooting

王佳丽, 刘慧欣, 岳康杰, 亢红伟, 乔珂, 田旭平. 圆柏组织培养体系的建立[J]. 园艺学报, 2026, 53(3): 867-880.

WANG Jiali, LIU Huixin, YUE Kangjie, KANG Hongwei, QIAO Ke, TIAN Xuping. Establishment of the Tissue Culture System of Sabina chinensis[J]. Acta Horticulturae Sinica, 2026, 53(3): 867-880.

图/表 13

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叶形 Leaf shape	时间/min Time	污染率/% Pollution rate	褐变率/% Browning rate	成活率/% Survival rate
鳞叶 Scale leaf	15	59.32 ± 10.35 a	40.68 ± 1.28 c	0 c
	16	41.33 ± 11.56 ab	58.67 ± 7.41 bc	0 c
	17	21.36 ± 9.79 b	72.25 ± 11.29 bc	6.39 ± 2.57 b
	18	8.52 ± 1.32 c	80.30 ± 11.72 ab	11.18 ± 1.42 a
	19	1.51 ± 0.19 c	98.49 ± 11.89 a	0 c
针叶 Needle leaf	15	21.66 ± 9.12 a	78.34 ± 12.29 b	0 b
	16	2.51 ± 0.31 b	93.80 ± 11.81 ab	3.69 ± 0.74 a
	17	0 b	100.00 ± 0.00 a	0 b
	18	0 b	100.00 ± 0.00 a	0 b
	19	0 b	100.00 ± 0.00 a	0 b

叶形 Leaf shape	时间/min Time	污染率/% Pollution rate	褐变率/% Browning rate	成活率/% Survival rate
鳞叶 Scale leaf	15	59.32 ± 10.35 a	40.68 ± 1.28 c	0 c
	16	41.33 ± 11.56 ab	58.67 ± 7.41 bc	0 c
	17	21.36 ± 9.79 b	72.25 ± 11.29 bc	6.39 ± 2.57 b
	18	8.52 ± 1.32 c	80.30 ± 11.72 ab	11.18 ± 1.42 a
	19	1.51 ± 0.19 c	98.49 ± 11.89 a	0 c
针叶 Needle leaf	15	21.66 ± 9.12 a	78.34 ± 12.29 b	0 b
	16	2.51 ± 0.31 b	93.80 ± 11.81 ab	3.69 ± 0.74 a
	17	0 b	100.00 ± 0.00 a	0 b
	18	0 b	100.00 ± 0.00 a	0 b
	19	0 b	100.00 ± 0.00 a	0 b

叶形 Leaf shape	种类 Type	成活率/% Survival rate	褐变率/% Browning rate
鳞叶 Scale leaf	MS	13.26 ± 1.46 bc	86.74 ± 11.58 ab
	1/2MS	26.35 ± 4.39 b	73.65 ± 10.41 b
	WPM	17.83 ± 2.96 b	82.17 ± 11.22 b
	White	4.85 ± 1.17 c	95.15 ± 9.69 a
	DCR	46.32 ± 4.69 a	53.68 ± 7.51 c
针叶 Needle leaf	MS	4.73 ± 1.22 bc	95.27 ± 10.09 ab
	1/2MS	8.67 ± 2.41 b	91.33 ± 9.77 b
	WPM	2.37 ± 0.85 c	97.63 ± 12.96 a
	White	0 c	100.00 ± 0.00 a
	DCR	60.84 ± 5.93 a	39.16 ± 6.51 c

叶形 Leaf shape	种类 Type	成活率/% Survival rate	褐变率/% Browning rate
鳞叶 Scale leaf	MS	13.26 ± 1.46 bc	86.74 ± 11.58 ab
	1/2MS	26.35 ± 4.39 b	73.65 ± 10.41 b
	WPM	17.83 ± 2.96 b	82.17 ± 11.22 b
	White	4.85 ± 1.17 c	95.15 ± 9.69 a
	DCR	46.32 ± 4.69 a	53.68 ± 7.51 c
针叶 Needle leaf	MS	4.73 ± 1.22 bc	95.27 ± 10.09 ab
	1/2MS	8.67 ± 2.41 b	91.33 ± 9.77 b
	WPM	2.37 ± 0.85 c	97.63 ± 12.96 a
	White	0 c	100.00 ± 0.00 a
	DCR	60.84 ± 5.93 a	39.16 ± 6.51 c

种类 Type	浓度/（mg · L^-1） Concentration	鳞叶Scale leaf		针叶Needle leaf
种类 Type	浓度/（mg · L^-1） Concentration	褐变率/% Browning rate	成活率/% Survival rate	褐变率/% Browning rate	成活率/% Survival rate
对照（Control）	0	51.83 ± 6.27 ab	48.17 ± 5.73 bc	40.19 ± 5.62 b	59.81 ± 7.19 bc
活性炭（AC）	500	48.22 ± 7.54 b	51.78 ± 6.24 b	32.47 ± 4.31 bc	67.83 ± 6.42 b
	1 000	43.89 ± 8.91 b	56.11 ± 8.86 b	30.49 ± 7.91 bc	69.51 ± 7.64 b
	2 000	37.18 ± 9.22 bc	62.82 ± 9.43 b	22.39 ± 9.66 bc	77.61 ± 6.91 b
	3 000	53.86 ± 10.49 ab	46.14 ± 7.81 bc	48.65 ± 7.62 ab	51.35 ± 8.61 bc
聚乙烯吡咯烷酮（PVP）	0.5	51.35 ± 12.23 b	48.65 ± 9.18 bc	19.65 ± 9.13 c	80.35 ± 7.93 ab
	1.0	8.19 ± 1.34 d	91.81 ± 8.66 a	18.21 ± 9.76 c	81.79 ± 6.44 ab
	2.0	17.91 ± 3.91 c	82.09 ± 8.04 ab	6.75 ± 1.13 d	93.25 ± 6.82 a
	3.0	19.83 ± 7.19 c	80.17 ± 11.51 ab	14.26 ± 2.45 c	85.74 ± 7.64 ab
硝酸银（AgNO₃）	0.5	63.41 ± 8.19 a	36.59 ± 6.33 c	44.19 ± 6.73 ab	55.81 ± 8.12 bc
	1.0	66.19 ± 7.33 a	33.81 ± 5.42 c	49.28 ± 7.85 a	50.72 ± 5.19 bc
	2.0	54.18 ± 8.62 ab	45.82 ± 4.39 bc	55.83 ± 6.77 a	44.17 ± 6.93 c
	3.0	42.31 ± 5.44 b	57.69 ± 5.73 b	37.24 ± 7.45 b	62.76 ± 5.91 b