高温和氟乐灵诱导绣球产生2n花粉

doi:10.16420/j.issn.0513-353x.2024-0950

园艺学报 ›› 2025, Vol. 52 ›› Issue (11): 3090-3102.doi: 10.16420/j.issn.0513-353x.2024-0950

高温和氟乐灵诱导绣球产生2n花粉

杨紫薇¹, 张华¹, 邱帅², 唐玉超¹, 傅学浩¹, 张嘎尘¹, 孙明¹^,^*(), 刘秀丽¹^,^*()

¹ 北京林业大学园林学院，花卉种质创新与分子育种北京市重点试验室，国家花卉工程技术研究中心，城乡生态环境北京实验室，园林环境教育部工程研究中心，林木花卉遗传育种教育部重点实验室，林木资源高效生产全国重点实验室，北京 100083
² 浙江省杭州市园林绿化股份有限公司，杭州 310020

收稿日期:2025-01-08 修回日期:2025-08-06 出版日期:2025-11-26 发布日期:2025-11-26
通讯作者:
^* E-mail：sunming@bjfu.edu.cn，
showlyliu@126.com
基金资助:
国家自然科学基金面上项目(31971708); 国家自然科学基金面上项目(32271947); 雄安新区科技创新专项(2022XAGG0100)

High Temperature and Trifluralin Induce 2n Pollen in Hydrangea macrophylla

YANG Ziwei¹, ZHANG Hua¹, QIU Shuai², TANG Yuchao¹, FU Xuehao¹, ZHANG Gachen¹, SUN Ming¹^,^*(), LIU Xiuli¹^,^*()

¹ State Key Laboratory of Efficient Production of Forest Resources， Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education，Engineering Research Center of Landscape Environment of Ministry of Education，Beijing Laboratory of Urban and Rural Ecological Environment，National Engineering Research Center for Floriculture，Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding，School of Landscape Architecture，Beijing Forestry University， Beijing 100083， China
² Hangzhou Landscaping Incorporated， Hangzhou 310020， China

Received:2025-01-08 Revised:2025-08-06 Published:2025-11-26 Online:2025-11-26

摘要/Abstract

摘要： 以平顶型大叶绣球‘紫夜’（Hydrangea macrophylla‘Ziye’）为材料，观测了小孢子母细胞减数分裂进程，并对不同减数分裂时期的花蕾进行氟乐灵和高温诱导处理，测定了不同处理的2n花粉诱导率和离体培养的2n花粉活力。结果显示，氟乐灵包裹处理在小孢子母细胞时期（pollen mother cell，PMC）诱导效果最佳，而高温诱导则在终变期—中期Ⅰ时效果最佳。0.1%氟乐灵 + 2%二甲基亚砜（DMSO）处理48 h的诱导效果最为理想，高温（40 ℃）处理6 h为最优。经两种方法诱导产生的2n花粉均显示出活力，适用于后续杂交试验。综上，氟乐灵和高温均能有效诱导2n花粉的形成，其中氟乐灵诱导更为高效。

关键词: 绣球, 2n花粉诱导, 染色体加倍, 氟乐灵, 高温诱导

Abstract:

This study observed the meiotic process of microsporoblasts using flat-topped Hydrangea macrophylla‘Ziye’as material，and then treated flower buds with trifluralin wrapping and high temperature treatments at different meiotic periods. This study subsequently determined the 2n pollen induction rate of the different treatments and the viability of the 2n pollen in vitro germination. The results demonstrated that the trifluralin wrapping treatment achieved the highest induction effect during the“pollen mother cell”stage，while high-temperature induction exhibited optimal efficacy from the“late meiotic phase to metaphaseⅠ”. In the trifluralin induction process，a 48 h exposure to 0.1% trifluralin in conjunction with 2% dimethyl sulfoxide yielded optimal results. For high-temperature induction，treatment at 40 ℃ for 6 h was the ideal condition. The 2n pollen produced by both induction methods retained viability，making it suitable for subsequent hybridization experiments. In conclusion，both trifluralin and high temperature treatments can successfully induce the formation of 2n pollen，with trifluralin being more efficient.

Key words: Hydrangea macrophylla, 2n pollen induction, chromosome doubling, trifluralin, high temperature induction

杨紫薇, 张华, 邱帅, 唐玉超, 傅学浩, 张嘎尘, 孙明, 刘秀丽. 高温和氟乐灵诱导绣球产生2n花粉[J]. 园艺学报, 2025, 52(11): 3090-3102.

YANG Ziwei, ZHANG Hua, QIU Shuai, TANG Yuchao, FU Xuehao, ZHANG Gachen, SUN Ming, LIU Xiuli. High Temperature and Trifluralin Induce 2n Pollen in Hydrangea macrophylla[J]. Acta Horticulturae Sinica, 2025, 52(11): 3090-3102.

图/表 12

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花蕾直径/mm Flower bud diameter	花药宽/mm Anther width	花药长/mm Anther length	花序状态 Morphology of inflorescence	减数分裂进程 Meiosis stage
1.30 ~ 1.70	0.30 ~ 0.60	0.50 ~ 0.60	不育花未张开、花梗伸长、小叶与花序有一定夹角 Decorative florets closed，pedicel elongating，bracts angled	小孢子母细胞 Pollen mother cell
1.70 ~ 1.90	0.50 ~ 0.70	0.60 ~ 0.70	不育花未张开、花梗伸长并张开、小叶基本打开 Decorative florets closed，pedicel spreading，bracts open	细线期—粗线期 Leptotene-pachytene
1.90 ~ 2.00	0.60 ~ 0.08	0.65 ~ 0.80	不育花膨大、花梗伸长、花蕾分散、小叶完全打开 Decorative florets swollen，pedicel elongate，buds loose，bracts fully open	终变期 Diakinesis
2.00 ~ 2.10	0.60 ~ 0.85	0.80 ~ 0.85	不育花开时打开、花梗进一步伸长、花蕾进一步分散Decorative florets opening，pedicel further elongate，buds more dispersed	中期Ⅰ—前Ⅱ Metaphase I-ProphaseⅡ
2.20 ~ 2.30	0.70 ~ 0.85	0.85 ~ 0.95	不育花进一步打开、花梗和花蕾进一步分散 Decorative florets fully open，pedicel and buds widely spaced	前Ⅱ—四分体时期 Prophase Ⅱ-Tetrad
> 2.30	0.85 ~ 0.90	0.95 ~ 1.00	不育花张开、小叶成长为正常叶片大小 Decorative florets open，bracts reach mature leaf size	成熟配子 Mature gametophyte

花蕾直径/mm Flower bud diameter	花药宽/mm Anther width	花药长/mm Anther length	花序状态 Morphology of inflorescence	减数分裂进程 Meiosis stage
1.30 ~ 1.70	0.30 ~ 0.60	0.50 ~ 0.60	不育花未张开、花梗伸长、小叶与花序有一定夹角 Decorative florets closed，pedicel elongating，bracts angled	小孢子母细胞 Pollen mother cell
1.70 ~ 1.90	0.50 ~ 0.70	0.60 ~ 0.70	不育花未张开、花梗伸长并张开、小叶基本打开 Decorative florets closed，pedicel spreading，bracts open	细线期—粗线期 Leptotene-pachytene
1.90 ~ 2.00	0.60 ~ 0.08	0.65 ~ 0.80	不育花膨大、花梗伸长、花蕾分散、小叶完全打开 Decorative florets swollen，pedicel elongate，buds loose，bracts fully open	终变期 Diakinesis
2.00 ~ 2.10	0.60 ~ 0.85	0.80 ~ 0.85	不育花开时打开、花梗进一步伸长、花蕾进一步分散Decorative florets opening，pedicel further elongate，buds more dispersed	中期Ⅰ—前Ⅱ Metaphase I-ProphaseⅡ
2.20 ~ 2.30	0.70 ~ 0.85	0.85 ~ 0.95	不育花进一步打开、花梗和花蕾进一步分散 Decorative florets fully open，pedicel and buds widely spaced	前Ⅱ—四分体时期 Prophase Ⅱ-Tetrad
> 2.30	0.85 ~ 0.90	0.95 ~ 1.00	不育花张开、小叶成长为正常叶片大小 Decorative florets open，bracts reach mature leaf size	成熟配子 Mature gametophyte

减数分裂时期 Meiotic stage	处理浓度（+ 2% DMSO）/% Treatment concentration	处理时间/h Treatment time	2n花粉诱导率/% Induction rate of 2n pollen
小孢子母细胞 Pollen mother cell	0.05	24	27.34 ± 1.66
		48	47.31 ± 7.11
		72	40.94 ± 1.65
	0.10	24	42.06 ± 1.93
		48	65.55 ± 6.40
		72	40.33 ± 7.42
	0.15	24	33.26 ± 9.05
		48	36.46 ± 2.45
		72	-
终变期—中期Ⅰ Diakinesis-metaphase Ⅰ	0.05	24	34.29 ± 7.50
		48	31.66 ± 5.44
		72	45.23 ± 11.81
	0.10	24	40.73 ± 3.05
		48	39.12 ± 5.42
		72	31.18 ± 9.27
	0.15	24	35.19 ± 5.76
		48	29.84 ± 10.60
		72	-

减数分裂时期 Meiotic stage	处理浓度（+ 2% DMSO）/% Treatment concentration	处理时间/h Treatment time	2n花粉诱导率/% Induction rate of 2n pollen
小孢子母细胞 Pollen mother cell	0.05	24	27.34 ± 1.66
		48	47.31 ± 7.11
		72	40.94 ± 1.65
	0.10	24	42.06 ± 1.93
		48	65.55 ± 6.40
		72	40.33 ± 7.42
	0.15	24	33.26 ± 9.05
		48	36.46 ± 2.45
		72	-
终变期—中期Ⅰ Diakinesis-metaphase Ⅰ	0.05	24	34.29 ± 7.50
		48	31.66 ± 5.44
		72	45.23 ± 11.81
	0.10	24	40.73 ± 3.05
		48	39.12 ± 5.42
		72	31.18 ± 9.27
	0.15	24	35.19 ± 5.76
		48	29.84 ± 10.60
		72	-

变异来源 Source of variation	df	F	P
减数分裂时期 Meiotic stage	1	8.61^*	0.006
处理浓度Treatment concentration	2	50.60^**	< 0.001
处理时间Treatment time	2	26.24^**	< 0.001
减数分裂时期 × 处理浓度Meiotic stage × treatment concentration	2	4.25^*	0.022
减数分裂时期 × 处理时间Meiotic stage × treatment time	2	10.63^**	< 0.001
处理浓度 × 处理时间Treatment concentration × treatment time	4	22.63^**	< 0.001
减数分裂 × 处理浓度 × 处理时间Meiotic stage × treatment concentration × treatment time	4	0.85	0.503

高温和氟乐灵诱导绣球产生2n花粉

High Temperature and Trifluralin Induce 2n Pollen in Hydrangea macrophylla

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不同浓度下氟乐灵处理 Treated with different concentrations of trifluralin treatment		不同时间下氟乐灵处理 Treated with different times of trifluralin treatment
处理浓度/% Treatment concentration	平均2n花粉诱导率/% Average induction rate of 2n pollen	处理时间/h Treatment time	平均2n花粉诱导率/% Average induction rate of 2n pollen
0.05	37.80 ± 9.42 b	24	35.48 ± 6.87 b
0.10	43.16 ± 12.07 a	48	41.65 ± 13.64 a
0.15	22.45 ± 17.28 c	72	26.28 ± 20.43 c

减数分裂时期 Meiotic stage	处理温度/℃ Treatment temperature	处理时间/h Treatment time	2n花粉诱导率/% Induction rates of 2n pollen
细线期—粗线期 Leptotene-pachytene	38	2	3.07 ± 0.93
		4	4.83 ± 2.49
		6	5.12 ± 0.75
	40	2	5.19 ± 0.62
		4	5.75 ± 1.29
		6	7.76 ± 1.12
	42	2	5.20 ± 0.25
		4	1.14 ± 0.91
		6	-
终变期—中期Ⅰ Diakinesis-metaphase Ⅰ	38	2	5.46 ± 1.47
		4	13.96 ± 1.91
		6	17.06 ± 3.53
	40	2	12.12 ± 1.47
		4	14.44 ± 3.92
		6	21.41 ± 2.83
	42	2	8.13 ± 1.43
		4	10.04 ± 0.71
		6	-

变异来源 Source of variation	df	F	P
减数分裂时期 Meiotic stage	1	205.70^**	< 0.001
处理温度Treatment temperature	2	64.97^**	< 0.001
处理时间Treatment time	2	7.91^**	0.001
减数分裂时期 × 处理温度Meiotic stage × treatment temperature	2	14.13^**	< 0.001
减数分裂时期 × 处理时间Meiotic stage × treatment time	2	9.25^**	< 0.001
处理温度 × 处理时间Treatment temperature × treatment time	4	26.11^**	< 0.001
减数分裂 × 处理温度 × 处理时间Meiotic stage × treatment temperature × treatment time	4	9.78^**	< 0.001

不同温度下高温处理 Treated with different temperature of high temperature treatment		不同时间下高温处理 Treated with different times of high temperature treatment
处理温度/℃ Treatment temperature	平均2n花粉诱导率/% Average induction rate of 2n pollen	处理时间/h Treatment time	平均2n花粉诱导率/% Average induction rate of 2n pollen
38	8.25 ± 5.69 b	2	6.52 ± 3.13 b
40	11.11 ± 6.12 a	4	8.36 ± 5.33 a
42	4.29 ± 3.95 c	6	7.88 ± 5.94 a

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高温和氟乐灵诱导绣球产生2n花粉

High Temperature and Trifluralin Induce 2n Pollen in Hydrangea macrophylla

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参考文献 46

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