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园艺学报 ›› 2026, Vol. 53 ›› Issue (1): 1-24.doi: 10.16420/j.issn.0513-353x.2024-0564

• 遗传育种·种质资源·分子生物学 •    下一篇

甜橙CYP71亚家族全基因组鉴定及CsCYP2的功能分析

王洪1, 万云清1, 戚鑫爱1, 盛慧子1, 任雅麟1, 刘梦雨1, 刘小丰1,*(), 赵晓春1,2,3,*()   

  1. 1 西南大学/中国农业科学院柑桔研究所国家柑桔工程技术研究中心, 重庆 400712
    2 西南大学宜宾研究院, 四川宜宾 644005
    3 西部(重庆)科学城种质创制大科学中心, 重庆 401329
  • 收稿日期:2025-06-04 修回日期:2025-10-24 出版日期:2026-01-25 发布日期:2026-01-26
  • 通讯作者:
    *E-mail:
  • 基金资助:
    国家重点研发计划项目(2021YFD1400801); 宜宾市双城协议保障科研经费科技项目(XNDX2022020008); 中央高校基本科研业务费专项资金资助(SWU221003)

Genome-Wide Identification of CYP71 Subfamily Genes in Citrus sinensis and Functional Analysis of CsCYP2

WANG Hong1, WAN Yunqing1, QI Xinai1, SHENG Huizi1, REN Yalin1, LIU Mengyu1, LIU Xiaofeng1,*(), ZHAO Xiaochun1,2,3,*()   

  1. 1 National Citrus Engineering Research CenterCitrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, China
    2 Yibin Academy of Southwest UniversityYibin, Sichuan 644005, China
    3 Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City, Chongqing 401329, China
  • Received:2025-06-04 Revised:2025-10-24 Published:2026-01-25 Online:2026-01-26

摘要:

CYP71基因是细胞色素P450酶家族中数量最多的一个亚家族,参与萜烯、类黄酮等次生代谢物的生物合成途径,特别是萜烯的羟基化和氧化等修饰,与植物代谢产物衍生物的产生密切相关。从甜橙基因组中鉴定到138个CYP71基因,其成员的保守基序与基因结构高度一致。启动子顺式作用元件分析表明,CsCYP71亚家族中具有光响应、激素响应等元件。通过转录组、基因表达、挥发性成分等对CsCYP71进行分析和鉴定,筛选到与精油含量明显相关的候选基因CsCYP2(Cs3g07330)。该基因在果皮中的表达水平与精油的积累显著正相关,且主要在油胞中表达,说明其可能与精油合成相关。在超表达和基因编辑的CsCYP2转基因植株中,CsCYP2的表达与含双氧单帖2-羟基桉树内酯(exo-2-Hydroxycineole)含量显著正相关,表明CsCYP2可能是氧化修饰合成exo-2-Hydroxycineole的关键基因。

关键词: 柑橘, CYP71亚家族基因, 氧化修饰, 精油, 萜烯衍生物

Abstract:

The CYP71 gene family is the largest subfamily within the cytochrome P450 enzyme family and plays a crucial role in the biosynthesis pathways of secondary metabolites such as terpenes and flavonoids. Specifically,it is involved in the hydroxylation and oxidation modifications of terpenes,closely associated with the production of plant metabolite derivatives. In the sweet orange genome,138 CYP71 genes have been identified,with highly conserved motifs and gene structures among its members. Promoter cis-acting element analysis revealed that the CsCYP71 subfamily contains light-responsive,hormone-responsive,and other regulatory elements. Through transcriptomic,gene expression,and volatile component analyses,the candidate gene Cs3g07330(termed as CsCYP2)was identified as significantly correlated with essential oil content. The expression level of this gene in leaves and peels showed a strong positive correlation with essential oil accumulation,and it was predominantly expressed in oil glands,suggesting its potential involvement in essential oil biosynthesis. In transgenic plants with CsCYP2 overexpression and gene editing,the expression of CsCYP2 was found to be highly positively correlated with the content of the dioxygenated monoterpene 2-hydroxycineole(exo-2-hydroxycineole). This indicates that CsCYP2 may be a key gene responsible for the oxidative modification in the synthesis of exo-2-hydroxycineole.

Key words: citrus, CYP71 subfamily gene, oxidative modification, essential oil, terpene derivatives