牡丹促成栽培中糖信号调控成花的机理研究

doi:10.16420/j.issn.0513-353x.2021-1250

园艺学报 ›› 2023, Vol. 50 ›› Issue (3): 596-606.doi: 10.16420/j.issn.0513-353x.2021-1250

牡丹促成栽培中糖信号调控成花的机理研究

薛玉前¹^,²^,^*, 柳志勇²^,^*, 孙凯荣², 张秀新², 吕英民¹^,^**(), 薛璟祺²^,^**()

¹北京林业大学园林学院，北京 100083
²中国农业科学院蔬菜花卉研究所，农业农村部园艺作物生物学与种质创制重点实验室，北京 100081

收稿日期:2022-11-25 修回日期:2023-01-13 出版日期:2023-03-25 发布日期:2023-04-03
通讯作者: **（E-mail：xuejingqi@caas.cn，luyingmin@bjfu.edu.cn）
作者简介:*共同第一作者
基金资助:
国家自然科学基金项目(31972440);国家自然科学基金项目(31972455);国家重点研发计划项目(2021YFE0110700);国家现代农业产业技术体系建设专项资金项目(CARS-21);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)

The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture

XUE Yuqian¹^,²^,^*, LIU Zhiyong²^,^*, SUN Kairong², ZHANG Xiuxin², LÜ Yingmin¹^,^**(), XUE Jingqi²^,^**()

¹College of Landscape Architecture，Beijing Forestry University，Beijing 100083，China
²Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture and Rural Affairs，Institute of Vegetables and Flower，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Received:2022-11-25 Revised:2023-01-13 Online:2023-03-25 Published:2023-04-03
Contact: **（E-mail：xuejingqi@caas.cn，luyingmin@bjfu.edu.cn）

摘要/Abstract

摘要：

以牡丹栽培品种‘洛阳红’为试材，在促成栽培条件下对花芽进行剥叶和涂抹赤霉素处理，探究其对花芽发育、叶片气孔特征、葡萄糖、果糖、蔗糖和海藻糖-6-磷酸（T6P）水平以及糖信号相关基因表达模式等的影响。结果表明，剥叶和赤霉素处理均有效促进牡丹花蕾发育且能够显著促进叶片气孔的发育，提高气孔的开度；剥叶和赤霉素处理能够促进蔗糖和葡萄糖的代谢，并通过促进T6P的积累触发糖信号，最终通过上调PsTPS1及抑制PsSnRK1和PsHXK1在叶片中的表达，诱导花蕾发育。

关键词: 牡丹, 促成栽培, 糖信号, 海藻糖-6-磷酸, 成花调控

Abstract:

In this study，tree peony cultivar‘Luoyang Hong’was used as the material. Under the conditions of forcing culture，the flower buds were defoliated and treated with gibberellin to explore the effects on the development of flower buds，leaf stomatal characteristics，and glucose，fructose，sucrose，trehalose-6-phosphate（T6P）levels and the expression patterns of genes related to sugar signaling pathway. The results showed that both defoliation and gibberellin treatments can effectively promote the development of flower buds and can significantly promote the development of stomata and increase the opening of the stomata；at the same time，defoliation and gibberellin treatments could promote the metabolic consumption of energy substances，such as sucrose and glucose. The sugar signaling pathway was triggered by sensing the accumulation of sugar signaling molecule T6P content，and finally it promoted the development of flower bud by up-regulating the expression of PsTPS1 in leaves and inhibiting the expression of PsSnRK1 and PsHXK1.

Key words: tree peony, forcing culture, sugar signal, T6P, flowering regulation

中图分类号:

S685.11

薛玉前, 柳志勇, 孙凯荣, 张秀新, 吕英民, 薛璟祺. 牡丹促成栽培中糖信号调控成花的机理研究[J]. 园艺学报, 2023, 50(3): 596-606.

XUE Yuqian, LIU Zhiyong, SUN Kairong, ZHANG Xiuxin, LÜ Yingmin, XUE Jingqi. The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture[J]. Acta Horticulturae Sinica, 2023, 50(3): 596-606.

图/表 7

表1 糖信号相关基因表达引物序列

Table 1 Primers sequences of sugar signaling-related genes for expression analysis

基因名称 Gene name	引物名称 Primer name	引物序列（5′-3′） Sequence	产物长度/bp Length of product
PsTPS1	qRTTPS1	F：CTCTCGACTTCCATGCCTTA；R：CTGTGATGTTCCAAGGGTTC	207
PsSnRK1	qRTSnRK1	F：CACGAAGGAATGGCGAATA；R：CAAGGAAGGCAGCACAAAG	211
PsHXK1	qRTHXK1	F：ATAAGAAAAGCCGTGGTAGAGC；R：AGTGTTCATACAAGCCACCATC	166
Actin	qRTActin	F：GAGAGATTCCGTTGCCCAG；R：TCCTTGCTCATTCTGTCTGC	191

图1 ‘洛阳红’牡丹剥叶和赤霉素处理对花蕾和叶片发育的影响

Fig. 1 Effect of defoliation and GA treatment on the development of bud and leaf of tree peony‘Luoyang Hong’

图2 ‘洛阳红’牡丹剥叶和赤霉素处理15 d 时叶片气孔的扫描电镜观察

Fig. 2 SEM micrograph cross-sections of tree peony‘Luoyang Hong’leaf stomata under partial defoliation and GA treatment on the 15th day

表2 ‘洛阳红’牡丹剥叶和赤霉素处理15 d时叶片气孔的特征

Table 2 Characteristics of leaf stomata of tree peony‘Luoyang Hong’under partial defoliation and GA treatment on the 15th day

处理 Treatment	气孔开度/μm Stomatal aperture	气孔密度/（No · mm^-2） Stomatal density	气孔长度/μm Stomatal length	气孔宽度/μm Stomatal width	气孔面积/μm² Stomatal area
对照Control	0.27 ± 0.21 c	251.85 ± 26.91 a	28.68 ± 2.81 a	23.58 ± 1.96 b	501.44 ± 31.66 c
剥叶Partial defoliation	0.55 ± 0.20 b	237.04 ± 36.29 a	28.96 ± 1.95 a	24.92 ± 1.31 a	559.46 ± 23.42 b
赤霉素GA	0.98 ± 0.47 a	181.49 ± 21.85 b	28.80 ± 2.20 a	25.69 ± 2.11 a	593.61 ± 26.55 a

图3 ‘洛阳红’牡丹促成栽培条件下叶片中蔗糖、葡萄糖、果糖和T6P含量的变化不同小字母表示在P < 0.05水平上显著差异，n = 3。

Fig. 3 The change of sucrose，glucose，fructose and T6P content at‘Luoyang Hong’tree peony under forcing culture Different letters indicate significant differences at P < 0.05 level，n = 3.

图4 不同植物中TPS1（A）、SnRK1（B）和HXK1（C）氨基酸序列进化树标尺表示不同植物TPS1、SnRK1或HXK1氨基酸序列之间的差异。

Fig. 4 Phylogenetic analysis of TPS1（A），SnRK1（B）and HXK1（C）amino acid in various plants The scale in represent the difference of every TPS1，SnRK1 and HXK1 amino acid from different plants.

图5 ‘洛阳红’牡丹剥叶和赤霉素处理对糖信号相关基因PsTPS1、PsSnRK1和PsHXK1在叶片中的表达水平的影响不同的小写字母表示同一时期不同处理间在0.05水平上差异显著（数据为平均数 ± 标准差，n = 3）。

Fig. 5 Effect of defoliation and GA treatment on the expression of PsTPS1，PsSnRK1，and PsHXK1 in leaves of tree peony‘Luoyang Hong’ The different lowercase letters indicated a significant difference among different treatment in expression of gene（Duncan’s test at P < 0.05 after analysis of variance；data are shown as mean ± SD，n = 3）.

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牡丹促成栽培中糖信号调控成花的机理研究

The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture

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牡丹促成栽培中糖信号调控成花的机理研究

The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture

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