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园艺学报 ›› 2019, Vol. 46 ›› Issue (6): 1134-1144.doi: 10.16420/j.issn.0513-353x.2018-0972

• 研究论文 • 上一篇    下一篇

桂花OfSVP响应环境低温对花芽分化的影响

朱益民,王千千,董 彬,张 超,赵宏波*   

  1. 浙江农林大学风景园林与建筑学院,浙江临安 311300
  • 出版日期:2019-06-25 发布日期:2019-06-25
  • 基金资助:
    国家自然科学基金项目(31501790);浙江省自然科学基金项目(LQ15C160004,Q16C160009);浙江省农业新品种选育重大科技专项(2016C02056-12)

Effect of OfSVP on Flower Bud Differentiation in Response to Ambient Temperature in Osmanthus fragrans

ZHU Yimin,WANG Qianqian,DONG Bin,ZHANG Chao,and ZHAO Hongbo*   

  1. School of Landscape Architecture and Architecture,Zhejiang A & F University,Lin’an,Zhejiang 311300,China
  • Online:2019-06-25 Published:2019-06-25

摘要: SVP(SHORT VEGETATIVE PHASE)是植物响应环境温度调控开花的一类MADS-box转录因子。为了探究桂花(Osmanthus fragrans)中SVP基因对其花芽分化和发育的作用,研究了19和25 ℃处理下‘堰虹桂’桂花的花芽分化过程以及SVP基因的表达特性。结果表明,19 ℃处理能够显著促进‘堰虹桂’花芽分化,最为显著的是缩短花序分化期和雌蕊分化期。qRT-PCR分析发现,桂花7个SVP基因(OfSVP1 ~ OfSVP7)在花芽不同发育阶段中均有表达,其中OfSVP4、OfSVP6、OfSVP7表达量从花序分化期开始一直呈现下降趋势,至雌蕊分化期达到最低,19 ℃处理的表达量一直低于25 ℃处理;19 ℃处理的OfSVP的下游基因FT、SOC1和GA20ox2的表达量基本上一直高于25 ℃处理,与OfSVP4、OfSVP6、OfSVP7表达呈负相关。因此,OfSVP4、OfSVP6、OfSVP7可能是桂花中响应低温促进花芽分化的关键基因,其可能通过调控FT、SOC1和GA20ox2的表达影响花芽分化进程。

关键词: 桂花, SVP, 环境低温, 花芽分化

Abstract: SVP(SHORT VEGETATIVE PHASE)is a kind of MADS-box transcription factor that regulates plant flowering in response to temperature. It plays an important role in flower bud differentiation. In order to explore the effect of SVP gene in Osmanthus fragrans on flower bud differentiation under different temperature treatments,we observed the anatomical structure of the flower bud differentiation process of Osmanthus fragrans‘Yabhong’under different temperatures (19 ℃ and 25 ℃). The results showed that compared with 25 ℃,the low temperature of 19 ℃ can significantly promote the flower bud differentiation process of Osmanthus fragrans‘Yabhong’,the most significant effects were inflorescence differentiation and pistil differentiation. qRT-PCR analysis showed that the seven OfSVP genes were expressed in different developmental stages of Osmanthus fragrans‘Yabhong’,and the expression of OfSVP4,OfSVP6,OfSVP7 began to decrease from the inflorescence differentiation stage and reached the lowest level in the pistil differentiation stage.,but the expression level of treatment at 19 ℃ was always lower than 25 ℃ treatment. The expression levels of downstream genes FT,SOC1 and GA20ox2 treated at 19 ℃ were basically higher than 25 ℃ treatment,negatively correlated with OfSVP4,OfSVP6,OfSVP7. Therefore,OfSVP4,OfSVP6,OfSVP7 may be a key gene in response to low temperature-induced flower bud differentiation in Osmanthus fragrans,which may affect the process of flower bud differentiation by regulating the expression of FT,SOC1 and GA20ox2.

Key words: Osmanthus fragrans, SVP, ambient temperature, flower bud differentiation

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