ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (8): 1565-1576.doi: 10.16420/j.issn.0513-353x.2019-0665

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QTL Mapping of Six Seed Yield Related Traits in Lotus

LIU Zhengwei1,GUO Dandan1,2,PENG Jing1,ZHU Honglian1,KUANG Jing1,JI Qun1,WANG Zhixin1,DING Yi3,*,and KE Weidong1,*   

  1. 1Wuhan Academy of Agricultural Sciences,Wuhan 430070,China;2College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan 430070,China;3College of Life Sciences,Wuhan University,Wuhan 430072,China
  • Online:2020-08-25 Published:2020-08-25

Abstract: In this study,a F2 population of lotus including 86 individuals was constructed with the cylindrical type accession‘Middle Lake Wild Lotus’and spherical type‘Chiang Mai Wild Lotus’as female and male parents,respectively. By combining the phenotypic identification of the F2 individuals and the parents in 2017 and 2018 with the genotyping by 197 SSR markers,QTL mapping of six yield-related traits(i.e.,black seed length,black seed width,single seed weight,flower number,carpel number and seed number per seedpod)was carried out. The results showed that all the six yield-related traits were close to continuous normal distribution in the F2 population,which indicated that these traits are quantitative traits controlled by multi genes. In total,twenty-eight QTLs that distributed on seven linkage groups were identified in the two years’ experiments,which explained 5.5%–27.4% of the phenotypic variation with LOD values between 2.55 and 12.34. Twenty-five QTLs(accounting for 89.29% of total QTLs)explained more than 10% phenotypic variation. Four of the twenty-five QTLs [i.e.,black seed length(bsl1),black seed width(bsw6)and flower number fn4.1,fn5)] were repeatedly identified over the two years,explaining 7.33% to 17.6% phenotypic variantion.

Key words: Nelumbo nucifera, lotus seed, yield trait, QTL mapping

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