Abstract: Genetic analysis and gene mapping were carried out on red mature fruit in cucumber（Cucumis sativus L.）using‘NCG127’（red mature fruit）and‘9930’（yellow mature fruit）as experimental materials in this study. The results showed that a single dominant nuclear gene，R，dominates the red mature fruit trait in cucumber. Simple sequence repeat（SSR）marker and bulked segregant analysis（BSA）were conducted on the DNA of F₂ plants，and resulted in screening 20 SSR markers linked to the R gene. The R gene was mapped to a linkage group corresponding to chromosome（Chr.）4 of cucumber. The flanking markers UW019319 and UW019203 were linked to the R gene with genetic distances of 0.8 and 0.7 cM，respectively. The physical distance between markers UW019319 and UW019203 was 213.4 kb based on the whole genome sequence of‘9930’cucumber line，and there were 30 candidate genes in this region.

Key words: cucumber, red mature fruit, genetic analysis, SSR marker, gene mapping