Molecular Identification on Mixed Infection of Tomato yellow leaf curl virus and Tomato chlorosis virus on Tomato in Jiangsu Province

doi:10.16420/j.issn.0513-353x.2015-0584

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (1): 89-99.doi: 10.16420/j.issn.0513-353x.2015-0584

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Molecular Identification on Mixed Infection of Tomato yellow leaf curl virus and Tomato chlorosis virus on Tomato in Jiangsu Province

WU Shu-hua1，LI Ting-fang1，ZHAO Wen-hao1，CHENG Zhao-bang1，GUO Qing-yun4，ZHAO Tong-min2，YU Wen-gui2，ZHU Ye-qin3,*，and JI Ying-hua1,*

1Institute of Plant Protection，Jiangsu Academy of Agricultural Sciences，Key Lab of Food Quality and Safety of Jiangsu Province – State Key Laboratory Breeding Base，Nanjing 210014，China；2Institute of Vegetable Crops，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China；3Jiangsu Station of Plant Protection，Nanjing 210036，China；4Institute of Plant Protection，Qinghai Academy of Agriculture and Forestry Sciences，Xining 810016，China

Online:2016-01-25 Published:2016-01-25

Abstract

Abstract:

In 2014，a routine survey of tomato yellow leaf curl disease in Jiangsu Province was carried out. Besides whitefly-transmitted geminiviruses，a whitefly-transmitted crinivirus was also detected in 17 tomato samples collected from Nanjing with symptoms of reduced leaf size or leaf curling and yellowing in the upper leaves and interveinal chlorosis and upward rolling and thickening in the lower leaves. Sequence analysis showed that the whitefly-transmitted geminivirus was an isolate of Tomato yellow leaf curl virus（TYLCV）and the whitefly-transmitted crinivirus was an isolate of Tomato chlorosis virus（ToCV）. The two viruses were also detected in Bemisia tabaci collected from the greenhouses.

Key words: Tomato yellow leaf curl virus, Tomato chlorosis virus, mixed infection, molecular detection

CLC Number:

S 641.2

WU Shu-hua1，LI Ting-fang1，ZHAO Wen-hao1，CHENG Zhao-bang1，GUO Qing-yun4，ZHAO Tong-min2，YU Wen-gui2，ZHU Ye-qin3,*，and JI Ying-hua1,*. Molecular Identification on Mixed Infection of Tomato yellow leaf curl virus and Tomato chlorosis virus on Tomato in Jiangsu Province[J]. ACTA HORTICULTURAE SINICA, 2016, 43(1): 89-99.

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Molecular Identification on Mixed Infection of Tomato yellow leaf curl virus and Tomato chlorosis virus on Tomato in Jiangsu Province

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