https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2002, Vol. 29 ›› Issue (5): 488-490.

• 研究报告 • 上一篇    下一篇

菊花18个品种的RAPD分析

秦贺兰*;游 捷;高俊平
  

  1. (中国农业大学观赏园艺与园林系, 北京100094)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-25 发布日期:2002-10-25

RAPD Analysis of 18 Chrysanthemum Cultivars

Qin Helan, You Jie, and Gao Junping
  

  1. (Department of Ornamental Horticulture and Landscape Architecture , China Agricultural University , Beijing100094 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-25 Published:2002-10-25

摘要: 采用RAPD 技术分析了18 个菊花品种DNA 的多态性。从80 个10 碱基随机引物中筛选出多态
性频率高的3 个引物。扩增的多态性片段在600 bp~1300 bp 之间。检测出两个品种特有的分子标记, ‘大红托桂’有OPD15 (1200 bp) ,‘玉翎管’缺失OPA17 (1100 bp) 。瓣型一致的品种间基因型相似系数较高。

关键词: 菊花, RAPD, 品种鉴定, 相似系数

Abstract: 18 cultivars of Chrysanthemum were analyzed using random amplified polymorphic DNAs (RAPDs) . Three primers out of 80 randomprimers showed high polymorphism , the polymorphic amplification fragment length ranged from 600 bp to 1300 bp. The 1200 bp fragment generated by primer OPD15 was present only in‘Dahongtuogui’, while the 1100 bp fragment generated by primer OPA17 was only absent in‘yulingguan’in 18 cultivars. The cultivars with similar petal type have high similarity coeficience (SC) among 18 cultivars.

Key words: Chrysanthemum, RAPD, Cultivars identification, Similarity coefficence

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