辣(甜) 椒细胞质雄性不育系减数分裂和雄配子发生过程

园艺学报 ›› 2004, Vol. 31 ›› Issue (6): 807-810.

辣(甜) 椒细胞质雄性不育系减数分裂和雄配子发生过程

王述彬^1,2;罗向东¹;戴亮芳¹;钱春桃¹;陈劲枫^1* ;刘金兵²;潘宝贵²

(¹ 南京农业大学作物遗传与种质创新国家重点实验室, 南京210095 ; ² 江苏省农业科学院蔬菜研究所, 南京210014)

收稿日期:2004-03-29 修回日期:2004-05-24 出版日期:2004-12-25 发布日期:2004-12-25

Meiotic Observation and Male Gametes Development in Cytoplasmic Male Sterile of Pepper ( Capsicum annuum L. )

Wang Shubin^{1, 2};Luo Xiangdong¹;Dai Liangfang¹;Qian Chuntao¹; Chen Jinfeng^1* ;Liu Jinbing²;Pan Baogui²

(¹ National Key Laboratory of Plant Genetic and Germplasam Enhancement , Nanjing 210095 , China ; ² Vegetable Research Institute ,Jiangsu Academy of Agricultural Sciences , Nanjing 210014 , China)

Received:2004-03-29 Revised:2004-05-24 Online:2004-12-25 Published:2004-12-25

摘要/Abstract

摘要：

运用减数分裂制片技术和石蜡切片法观察了辣椒细胞质雄性不育系21A 和保持系21B 以及甜
椒细胞质雄性不育系金A 和保持系金B 减数分裂和雄配子发生的整个过程。结果表明, 不育系和保持系都能进行正常减数分裂, 中期I 的染色体构型为9 个环状二价体和3 个棒状二价体, 后期I 和后期II 都均等分裂, 形成了正常的四分孢子(n = 12) 。保持系的四分孢子经过单核和双核期后形成成熟花粉粒。雄性不育系从小孢子单核靠边期开始异常, 绒毡层细胞径向异常膨大, 挤压花粉粒, 致使小孢子在形成双核之前完全裂解, 导致不育。

关键词: 辣椒, 细胞质雄性不育, 减数分裂, 雄配子发生

Abstract: The meiotic behavior and male gametes development in cytoplasmic male sterile lines (CMS) 21A and Jin A of hot and sweet pepper ( Capsicum annuum L. ) and their maintainer lines 21B and Jin B were studied in this paper. The results showed that microsporogenesis could process normally until former uni2nucleate pollen stage. There are not differences in the meiosis of both A lines and B lines , and between CMS of hot and sweet pepper. At metaphase I , the chromosome constitution was composed of nine ring bivalents and three rod bivalents. Equal chromosome divisions resulted in two poles with chromosome n=12 at anaphase I , and four poles at anaphaseII. After tetrad stage , uni-nucleate pollen underwent a progressive process of collapse , owing to being squeezed by bnormal swelled tapetal cells. Bi-ucleate pollen could not produce since the second pollen mitosis failed to occur ,and the gametes became completely sterile.

Key words: Pepper, Cytoplasmic male sterile, Meiosis, Male gametes development

中图分类号:

S 643.1

王述彬;罗向东;戴亮芳;钱春桃;陈劲枫;刘金兵;潘宝贵. 辣(甜) 椒细胞质雄性不育系减数分裂和雄配子发生过程[J]. 园艺学报, 2004, 31(6): 807-810.

Wang Shubin;;Luo Xiangdong;Dai Liangfang;Qian Chuntao;Chen Jinfeng;Liu Jinbing;Pan Baogui. Meiotic Observation and Male Gametes Development in Cytoplasmic Male Sterile of Pepper ( Capsicum annuum L. )[J]. ACTA HORTICULTURAE SINICA, 2004, 31(6): 807-810.

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