园艺学报 ›› 2019, Vol. 46 ›› Issue (12): 2347-2358.doi: 10.16420/j.issn.0513-353x.2019-0118

• 研究论文 • 上一篇    下一篇

菜瓜 × 马泡瓜遗传连锁图谱构建及果实相关性状QTL分析

王晨晖,栾非时*,高 鹏*,刘 识,解志强   

  1. 东北农业大学园艺园林学院,农业农村部东北地区园艺作物生物学与种质创制重点实验室,哈尔滨 150030
  • 出版日期:2019-12-25 发布日期:2019-12-25
  • 基金资助:

Construction of Melon Genetic Linkage Map and QTL Analysis of Fruit Related Traits in Snake Melon × Wild Type Melon Genetic Background

WANG Chenhui,LUAN Feishi*,GAO Peng*,LIU Shi,and XIE Zhiqiang   

  1. College of Horticulture and Landscape Architecture,Northeast Agricultural University;Ministry of Agriculture Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in Northeast China,Harbin 150030,China
  • Online:2019-12-25 Published:2019-12-25

摘要: 试验用果实相关性状差异显著的两个亲本,菜瓜(Cucumis melo ssp. melo var. flexuosus)品系M4-75(长型、肉厚)为母本和马泡瓜(C. melo ssp. agrestis)品系X207(小果、圆形、肉薄)为父本,进行基因组重测序,并以此双亲配制F2群体,进行CAPS标记的开发、遗传连锁图谱的构建和甜瓜果实相关性状的QTL分析。共筛选出180对具有多态性的引物,并得到一张遗传连锁图谱,该图谱包含165个CAPS标记、12个连锁群,覆盖基因组长度1 019.81 cM,标记间平均距离为6.18 cM。试验结果表明甜瓜果实相关性状均为数量性状,并检测到与之相关的QTL共14个,分布在第1、2、3、4、5、6、7、10和11连锁群上,LOD值介于2.67 ~ 9.63,可解释7.60% ~ 35.18%的表型变异率,其中果实形状相关位点有9个,果肉厚度相关位点有3个,pH相关位点有2个。

关键词: 甜瓜, 果实长度, CAPS标记, 遗传连锁图谱, QTL

Abstract: Based on the importance on the fruits related traits on melon production,QTLs locus linked with the fruits related traits in melon was analyzed in this paper. F2 populations obtained from Snake melon(Cucumis melo L.)‘M4-75’and wild type melon(Cucumis melo L.)‘X207’as parents were used as materials. Cleaved amplified polymorphic sequence(CAPS)markers were developed to construct a genetic linkage map and detect the QTLs(quantitative trait locus)related with fruit traits in melon based on the parental genome re-sequencing data. As the results,totally 180 CAPS markers were screened which showed polymorphism between parents and for the genetic linkage map construction. Finally,the map contained 165 markers and grouped into 12 linkage groups corresponded to the 12 chromosomes in melon,spanning a total length of 1 019.81 cM,with an average of 6.18 cM between markers. Meanwhile,fruits related traits(fruit shape,flesh thickness and flesh pH)in melon were all quantitative traits. Totally 14 QTLs associated with the traits were detected which distributed among Chromosome 1,2,3,4,5,6,7,10,11. The LOD(log of odds)score ranged from 2.67 to 9.63,which could explain the phenotypic variation rate of 7.60%–35.18%. Nine QTLs related with fruit shape,3 QTLs for flesh thickness and 2 QTLs for flesh pH.

Key words: melon, fruit length, CAPS marker, genetic linkage map, QTL