Changes of Total Phenol Content and the Activities of PPO, POD and PAL during the Browning in Phalaenopsis Explant in Vitro

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (3): 671-674.

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Changes of Total Phenol Content and the Activities of PPO, POD and PAL during the Browning in Phalaenopsis Explant in Vitro

Xu Chuanjun^{1, 2};Li Ling^1*

(¹College of Life Science, South China Normal University, Guangdong Key Laboratory of Biotechnology for Plant Development, gangzhou, Guangdong 510631, China; ²Fujian Institute of Subtropical Botany, Xiamen, Fujian 361006, China)

Received:2005-05-18 Revised:2005-09-07 Online:2006-06-25 Published:2006-06-25

Abstract

Abstract: In the early stage of Phalaenopsis explant browning, the activities of PPO and POD increased, while in the later stage, their activities declined. The PPO and POD isoenzyme showed that no PPO band appeared in 0 day culture, while a weak band of POD appeared. After 2 days culture, there were three bands of
POD appeared, and the band Ⅰappeared after cultured for 4 days and dispersed in another day; and three bands of PPO isoenzyme in browning cell appeared and the band Ⅲ had a higher activity when cultured for 4 days, the activitives of PPO and POD declined whit the culture extension. As explant turned black, the content of total phenol compounds and the activity of PAL increased and there was a extremely positive correlation between them.

Key words: Phalaenopsis, PPO, POD, PAL, Exp lant browning, Total phenol content

Xu Chuanjun;;Li Ling. Changes of Total Phenol Content and the Activities of PPO, POD and PAL during the Browning in Phalaenopsis Explant in Vitro[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 671-674.

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Changes of Total Phenol Content and the Activities of PPO, POD and PAL during the Browning in Phalaenopsis Explant in Vitro

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