Screening and RAPD Analysis of Gummy Stem Blight Resistance in Melon Germplasm

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (5): 1201-1206.

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Screening and RAPD Analysis of Gummy Stem Blight Resistance in Melon Germplasm

ZHOU Xiao-hui¹; Joseph N. Wolukau¹; LI Ying¹; ZHANG Yong-bin^1,2;WU Ming-zhu²;CHEN Jin-feng^1*

(¹State Key Laboratory of Plant Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;²Hamigua Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi830000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

Abstract

Abstract: Two hundred and eight accessions of melon germplasm were screened and evaluated for resistance to gummy stem blight (GSB) by artificial inoculation at the seedling stage. The results showed that eighty six among them were resistant and one hundred and twenty two were susceptible. Fourteen RAPD primers were used to analyze the genetic diversity of the twenty nine accessions with the most GSB resistance. In total one hundred and sixteen DNA polymorphic bands were scored, with an average of 8.29 bands for each primer. Shannons information index (I) and Neis gene diversity index were 0.4331 and 0.2855, respectively, indicating rather large genetic distances among those germplasm. Cluster analysis by UPGMA divided these twenty nine accessions into two groups, and the accessions with the same geographical origin were clustered together.

Key words: Melon, Gummy stem blight, Resistance, RAPD, Genetic diversity

ZHOU Xiao-hui;Joseph N. Wolukau;LI Ying;ZHANG Yong-bin;WU Ming-zhu;CHEN Jin-feng. Screening and RAPD Analysis of Gummy Stem Blight Resistance in Melon Germplasm[J]. ACTA HORTICULTURAE SINICA, 2007, 34(5): 1201-1206.

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Screening and RAPD Analysis of Gummy Stem Blight Resistance in Melon Germplasm

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