Abstract: To provide an important reference for further clarifying the molecular mechanism of fruitsoftening，GUS histochemical staining method was employed to detect tissue specific regulation of the CmACOⅠ promoter in melon（Cucumis melo L.）. The plant expression vector with a fusion gene，CmACOⅠ promoter and GUS gene was established and melon‘GT-1’was transformed by using Agrobacterium-mediated transformation system. Then the roots，stems，leaves，flowers，fruits and other organs in transformed plants during different growth stages were dyed with X-Gluc. The transformed plants with Kanamysin resistance and positive detection by PCR were obtained. It showed that some blue spots in the anther tissue and peel organ of the after-ripening fruit in the plants with a fusion gene （CmACOⅠ promoter and GUS gene）were detected by using X-Gluc dye，while the other organs did not appear blue spots. It showed that GUS gene was driven by melon CmACOⅠ promoter and expressed specifically in anther tissue and peel organ of the after-ripening fruit.

Key words: melon, CmACOⅠ promoter, GUS gene, tissue specificity