Abstract: In vitro culture techniques of tree peonies have yet been sued in micropropagation，somaclone and gene engineering breeding since first paper see in 1984. The challengings are still the low rate of shoot rooting and survive of plantlet transplanting and acclimatization. In this paper，review the two of five major stages in vitro culture of the tree peonies accordance with plant tissue culture practical. Focus on the impacts of shoot rooting in vitro and the survive factors in transplanting and acclimatization，reveal the effective ways and methods to increase rooting and transplanting efficiency. Discussed histological，physiological，biochemical and molecular aspects to resolve the problems in the tree peony tissue culture.

Key words: Paeonia suffruticosa, in vitro, tissue culture, shoot rooting, transplanting and acclimatization