Abstract: The influence of in vitm microenvimnment during rooting stage on the growth and physiology of strawberry(Fragaria al2aglt1．a Duch ‘Fenxiang’)plandets during ex vitm acclinkatization was studied．Nodal cuttings were cultured on solid Murashige—Skoog mediuin with 3％sucrE6e or without sueI'of，and in low (60+10 tanol·m．s )or high(150~10 pmol‘m^-2．s )photosynthetic photon flux densities，and normal(350±20 t,-L‘L )or elevated (700±20 t,-L·L )C0₂ concentration．After 20 days of rooting culture，planflets were transferred to the greenhouse for a month period of acclimatization．Th e results indicate that 3％ sucrE6e is be neficial to bimmas forrmtion of planflets，higher PPFD and elevated C02 concentration were both of benefit to the growth of anflets．But higher PPFD reduced the c~omphyH content，and a slight negative efect on photosynthesis could be found in the culture conditions of 3％ sui'ose without elevated C02．During the acclima tization，photoinhibition was not obvious，and pPSI1 was incnxIsed．Th e distinctive efects of the diferent in vitro culture conditions on growth．pigments and photosynthetic parameters were decnxIsed during greenhouse acclimatization．Th erefore．we could conclude that pmper sucn)se concentration ma y promote the biomass forrmtion and weaken the sensibility to the envimnnnt variable．and properly elevated C0₂ and PPFD may be helpful to the growth of plantlets．

Key words: Strawbe rry, Micmpmpagation, Microenvimnment, Photoautotrophy, Photomixotrophy