Cloning and Sequence Analysis of Beta-1,3-glucanase Gene cDNA in Brassicacampestris ssp. chinensis

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (5): 670-672.

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Cloning and Sequence Analysis of Beta-1,3-glucanase Gene cDNA in Brassicacampestris ssp. chinensis

Wang Yanhua^1,2;Hou Xilin¹*;Shi Gongjun¹

(¹ National Key Laboratory of Crop Genetics and Germplasm Enhancement , Nanjing Agricultural University , Nanjing 210095 , China ; ² College of Horticulture , Hebei Agricultural University , Baoding 071001 , China)

Received:2004-01-12 Revised:2004-04-01 Online:2004-10-25 Published:2004-10-25
Contact: Hou Xilin

Abstract

Abstract: The beta-1,3-glucanase gene cDNA sequence were cloned using RT-PCR with cDNA isolated f rom Brassica campestris ssp. chi nensis‘Xuekeqing’, which displayed resistances to downy mildew.Sequencing result indicated that cloned f ragment of beta21032glucanase gene encode a peptide containing 343amino acids. The further comparison to turnip beta-1,3-glucanase ( bg1) gene and Arabidopsis thaliana beta-1,3-glucanase ( bg2) gene showed that identity was 98 % and 84 % , respectively. As a result , the sequence was accepted and released by GenBank (accession number AY395720) .

Key words: Brassica campestris ssp. chinensis Makino, Downy mildew, Pathogenesis-related proteins, Beta-1, 3-glucanase gene

CLC Number:

S 634.3

Wang Yanhua;Hou Xilin;Shi Gongjun. Cloning and Sequence Analysis of Beta-1,3-glucanase Gene cDNA in Brassicacampestris ssp. chinensis[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 670-672.

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Cloning and Sequence Analysis of Beta-1,3-glucanase Gene cDNA in Brassicacampestris ssp. chinensis

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