Abstract: An efficient plant regeneration protocol for Rhaphiolepis indica L. was achieved via organogenesis direct formation on stem segment explants in vitro. Several factors affecting in vitro regeneration of R. indica, such as basic medium, different hormones combination, concentrations of sucrose and AgNO3, were examined. The optimum medium for shoot regeneration was MS medium containing 2.0 mg·L^-1 6-BA, 0.5 mg·L^-1 NAA, 1.0 mg·L^-1 AgNO₃ and 40 g·L^-1 sucrose. 90% of adventitious shoot regeneration frequency with 5.38 shoots per explants was obtained. Excised shoots were effectively elongated in 1/2 MS medium. Elongated shoots of 3.0 cm rooted when they were transferred to 1/2MS medium containing1.0 mg·L^-1 NAA after 40 days and developed into healthy plantlets, which resulted in a rooting rate of 100%.

Key words: Rhaphiolepis indica L., stem segment, plant growth regulator, shoot regeneration