Embryogenesis and Plant Regeneration of Chinese Kale via Isolated Microspore Culture

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (2): 239-240.

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Embryogenesis and Plant Regeneration of Chinese Kale via Isolated Microspore Culture

He Hangjun;Wang Xiaowu;Wang Bingliang

( Institute of Vegetables and Flowers，Chinese Academy of Agricuhural Science，Beijing 100081，China； Department of Horticulture，Zhejiang University，Hangzhou 310029，China )

Received:2003-04-08 Revised:2003-06-12 Online:2004-04-25 Published:2004-04-25

Abstract

Abstract: Isolated microspores of 5 Chinese kale genotypes were cultured． It was found that only those late-uninucleate and binucleate microspores could grow into division． The results also showed that genotypes had important impact on embryoid induction． Effect of shaking culture on embryoids development was examined． The results demonstrated that slow shaking could promote development of embryoids and increase the proportion of cotyledonary embryoids．MS and B5 media showed no difference on plant
regeneration．

Key words: Chinese kale, Isolated mierospore, Embryoid, Shaking culture, Plant regeneration

CLC Number:

S 635

He Hangjun;Wang Xiaowu;Wang Bingliang. Embryogenesis and Plant Regeneration of Chinese Kale via Isolated Microspore Culture[J]. ACTA HORTICULTURAE SINICA, 2004, 31(2): 239-240.

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Embryogenesis and Plant Regeneration of Chinese Kale via Isolated Microspore Culture

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