Abstract:

To determine the viruses infecting flat nectarine in Wuhan，Hubei，five shoot samples with the virus-like symptoms were collected and analyzed by small RNA sequencing. The results showed that peach associated luteovirus（PaLV），peach virus D（PeVD），and peach latent mosaic viroid（PLMVd）were detected. RT-PCR with specific primers was conducted to confirm the result. Partial sequences of PaLV，PeVD and PLMVd were obtained by RT-PCR，sequenced and deposited in GenBank. Sequences of PaLV，PeVD and PLMVd（GenBank accession No. OP244073，OP244074 and OP244075）were 92.15%-98.59% identical with the corresponding nucleotide sequences of PaLV isolate T01-2（MK361479），PeVD isolate T02-1（MK361476）and PLMVd isolate HUEI-Ob166/1-1（MW046337） in GenBank. One hundred and nine flat nectarine samples were collected and detected by RT-PCR with primers specific for PaLV，PeVD，PLMVd and six other peach viruses. Only PLMVd，PaLV and PeVD were detected in these samples，with a detection rate of 33.94%，29.36% and 5.50%，respectively. The highest incidence of PLMVd indicated that it was the dominant viroid infecting flat nectarine in Wuhan. The mixed infection of viruses and viroid was 22.93%，higher than that of a single virus or viroid infection，indicating that mixed infection of virus and viroid was common in flat nectarine.

Key words: flat nectarine, virus, viroid, small RNA sequencing, RT-PCR