Identification of Pepper Virus Diseases and Analysis of BWYV Genetic Variation in Dingxi Area of Gansu Province

doi:10.16420/j.issn.0513-353x.2024-0282

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (2): 491-502.doi: 10.16420/j.issn.0513-353x.2024-0282

• Plant Protection • Previous Articles Next Articles

Identification of Pepper Virus Diseases and Analysis of BWYV Genetic Variation in Dingxi Area of Gansu Province

YANG Wenqing, WANG Jiahui, XU Bingliang, LI Huixia, LIANG Qiaolan, YANG Shunyi^*(), NIU Erbo^*()

College of Plant Protection，Gansu Agricultural University，Anning，Gansu 730070，China

Received:2024-05-08 Revised:2024-11-07 Online:2025-02-25 Published:2025-02-23
Contact: YANG Shunyi, NIU Erbo

Abstract

Abstract:

Using a method that combines high-throughput sequencing technology with RT-PCR，pathogen identification was conducted on pepper leaves infected with viral diseases in the Dingxi area of Gansu Province. The results indicated that the infected leaves were co-infected with cucumber mosaic virus and sugar beet western yellow virus. Notably，this study marks the first identification of BWYV infecting peppers in Gansu. Specific primers were employed to clone the coat protein and movement protein genes of BWYV，facilitating an in-depth analysis of the molecular evolution and genetic variation of this virus. Sequence comparisons revealed that the nucleotide and amino acid similarities between the coat protein（CP）gene of BWYV and isolates from other species ranged from 93.27% to 99.51% and from 90.10% to 99.01%，respectively. The similarities of the movement protein（MP） genes were found to be between 89.20% and 99.05% for nucleotides and between 86.86% and 98.86% for amino acids. Furthermore，phylogenetic analysis of BWYV demonstrated that different isolates could be categorized into four distinct groups. These groups exhibited no discernible geographical distribution characteristics，while host plant diversity analysis revealed significant correlations with host species.

Key words: pepper, beet western yellows virus, RT-PCR detection, evolutionary analysis

YANG Wenqing, WANG Jiahui, XU Bingliang, LI Huixia, LIANG Qiaolan, YANG Shunyi, NIU Erbo. Identification of Pepper Virus Diseases and Analysis of BWYV Genetic Variation in Dingxi Area of Gansu Province[J]. Acta Horticulturae Sinica, 2025, 52(2): 491-502.

Figures/Tables 13

References 38

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引物名称 Primer	引物序列（5′-3′） Primer sequence	产物/bp Fragment	退火温度/℃ T_m	参考文献 Reference
CMV-CP	F：GTCGACCATGGACAAAT；R：CCGGGTCAGACTGGTAGCA	660	56	王德富等,2016
CMV-MP-	F：AGGCATGGCTTTCCAAGGTACCAG；R：AGTGCTAAAGACCGTTAACCA	850	55	杜江等,2023
BWYV-CP	F：CCTCAAAATCTCAGCCCACG；R：TGGCATCATCGGTACTCTGG	780	60	本试验This study
BWYV-MP	F：TGAATACGGTCGTGGGTAGA；R：GGCCATTATACGAGTCGCTG	725	56	本试验This study

引物名称 Primer	引物序列（5′-3′） Primer sequence	产物/bp Fragment	退火温度/℃ T_m	参考文献 Reference
CMV-CP	F：GTCGACCATGGACAAAT；R：CCGGGTCAGACTGGTAGCA	660	56	王德富等,2016
CMV-MP-	F：AGGCATGGCTTTCCAAGGTACCAG；R：AGTGCTAAAGACCGTTAACCA	850	55	杜江等,2023
BWYV-CP	F：CCTCAAAATCTCAGCCCACG；R：TGGCATCATCGGTACTCTGG	780	60	本试验This study
BWYV-MP	F：TGAATACGGTCGTGGGTAGA；R：GGCCATTATACGAGTCGCTG	725	56	本试验This study

基因登录号 Gene ID	株系 Strain	寄主植物 Host plant	地理来源 Geographical origin	核苷酸 Nucleotide	氨基酸 Aminoacid
MK307779.1	SDJN16	辣椒Capsicum annuum	中国山东Shandong，China	99.18	98.51
MK307780.1	SDWF16	辣椒Capsicum annuum	中国山东Shandong，China	99.01	99.01
LC428355.1	S14	菠菜Spinacia oleracea	日本Japan	99.51	99.01
MW349136.1	JJ18	辣椒Capsicum annuum	韩国Korea	99.18	98.51
OQ570952.1	YA35	萝卜Raphanus sativus	韩国Korea	99.18	98.02
LC592344.1	ADHS	北玄参Scrophularia buergeriana	韩国Korea	99.01	99.01
LC428357.1	R3a	萝卜Raphanus sativus	日本Japan	98.85	98.02
OM419176.1	—	香菜Coriandrum sativum	塞浦路斯Cyprus	93.92	91.09
ON924237.1	W11	甜菜Beta vulgaris	美国USA	93.76	91.58
KU521325.1	Rouen1	猪笼草Nepenthes mirabilis	法国French	93.60	90.10
ON924244.1	NYC	甜菜Beta vulgaris	美国USA	93.60	91.58
NC004756.1	USA	甜菜Beta vulgaris	美国USA	93.60	92.08
HM804471.1	BJ^A	甜菜Beta vulgaris	中国北京Beijing，China	97.87	98.51
HM804472.1	BJ^B	甜菜Beta vulgaris	中国北京Beijing，China	95.73	96.04
EU636991.1	IM	甜菜Beta vulgaris	中国内蒙古Inner Mongolia，China	99.18	90.10
EU636990.1	GS	甜菜Beta vulgaris	中国甘肃Gansu，China	93.76	93.56
AB903037.1	KY	菠菜Spinacia oleracea	日本Japan	93.27	94.55

基因登录号 Gene ID	株系 Strain	寄主植物 Host plant	地理来源 Geographical origin	核苷酸 Nucleotide	氨基酸 Aminoacid
MK307779.1	SDJN16	辣椒Capsicum annuum	中国山东Shandong，China	99.18	98.51
MK307780.1	SDWF16	辣椒Capsicum annuum	中国山东Shandong，China	99.01	99.01
LC428355.1	S14	菠菜Spinacia oleracea	日本Japan	99.51	99.01
MW349136.1	JJ18	辣椒Capsicum annuum	韩国Korea	99.18	98.51
OQ570952.1	YA35	萝卜Raphanus sativus	韩国Korea	99.18	98.02
LC592344.1	ADHS	北玄参Scrophularia buergeriana	韩国Korea	99.01	99.01
LC428357.1	R3a	萝卜Raphanus sativus	日本Japan	98.85	98.02
OM419176.1	—	香菜Coriandrum sativum	塞浦路斯Cyprus	93.92	91.09
ON924237.1	W11	甜菜Beta vulgaris	美国USA	93.76	91.58
KU521325.1	Rouen1	猪笼草Nepenthes mirabilis	法国French	93.60	90.10
ON924244.1	NYC	甜菜Beta vulgaris	美国USA	93.60	91.58
NC004756.1	USA	甜菜Beta vulgaris	美国USA	93.60	92.08
HM804471.1	BJ^A	甜菜Beta vulgaris	中国北京Beijing，China	97.87	98.51
HM804472.1	BJ^B	甜菜Beta vulgaris	中国北京Beijing，China	95.73	96.04
EU636991.1	IM	甜菜Beta vulgaris	中国内蒙古Inner Mongolia，China	99.18	90.10
EU636990.1	GS	甜菜Beta vulgaris	中国甘肃Gansu，China	93.76	93.56
AB903037.1	KY	菠菜Spinacia oleracea	日本Japan	93.27	94.55

登录号 Gene ID	株系 Strain	寄主植物 Host plant	地理来源 Geographical origin	核苷酸 Nucleotide	氨基酸 Aminoacid
MK307780.1	SDWF16	辣椒Capsicum annuum	中国山东Shandong，China	99.05	98.86
LC428357.1	R3a	萝卜Raphanus sativus	日本Japan	99.05	98.86
OQ625515.1	NJ22	萝卜Raphanus sativus	韩国Korea	99.05	98.86
LC592344.1	ADHS	北玄参Scrophularia buergeriana	韩国Korea	97.92	94.86
ON759519.1	P03	辣椒Capsicum annuum	韩国Korea	98.86	98.29
MK307779.1	SDJN16	辣椒Capsicum annuum	中国山东Shandong，China	98.67	98.29
OQ570952.1	YA35	萝卜Raphanus sativus	韩国Korea	98.48	98.29
KM076647.1	LS	益母草Leonurus sibiricus	韩国Korea	98.48	97.14
HM804471.1	BJ^A	甜菜Beta vulgaris	中国北京Beijing，China	96.21	93.14
HM804472.1	BJ^B	甜菜Beta vulgaris	中国北京Beijing，China	96.02	92.57
ON924175.1	JJ	小麦Triticum aestivum	中国江苏Jiangsu，China	91.29	89.14
EU636990.1	GS	甜菜Beta vulgaris	中国甘肃Gansu，China	89.96	87.43
HQ543079.1	6pCP	豌豆Pisum sativum	澳大利亚Australia	89.39	89.14
EU636991.1	IM	甜菜Beta vulgaris	中国内蒙古Inner Mongolia，China	89.58	86.86
HQ543082.1	9p	豌豆Pisum sativum	澳大利亚Australia	89.39	88.00
HQ543095.1	27p	豌豆Pisum sativum	澳大利亚Australia	89.20	86.86
HQ543084.1	14p	豌豆Pisum sativum	澳大利亚Australia	89.20	87.43

Identification of Pepper Virus Diseases and Analysis of BWYV Genetic Variation in Dingxi Area of Gansu Province

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分组 Group	Ⅰ	Ⅱ	Ⅲ	Ⅳ
Ⅰ	—	—	—	—
Ⅱ	0.0464	—	—	—
Ⅲ	0.1157	0.1367	—	—
Ⅳ	0.1212	0.1470	0.1482	—

分组Group	Ⅰ	Ⅱ	Ⅲ	Ⅳ
Ⅰ	—	—	—	—
Ⅱ	0.0889	—	—	—
Ⅲ	0.1905	0.1981	—	—
Ⅳ	0.2021	0.2229	0.1652	—

[1]	CAI Zeyan, ZHANG Mingxing, ZHOU Chi, TAO Yu, YANG Sha, LI Xin, LI Xuefeng. Analysis of Endogenous Microbial Community Diversity，Structure and Function of Pepper Different Resistant Phytophthora Blight Cultivars [J]. Acta Horticulturae Sinica, 2025, 52(2): 322-336.
[2]	ZOU Xuexiao, YANG Sha, DAI Xiongze, HU Bowen, XU Hao, ZHU Fan, PEI Songyu, YUAN Fang. The Rapid Development of China’s Chili Pepper Industry Over the Past 40 Years [J]. Acta Horticulturae Sinica, 2025, 52(1): 247-258.
[3]	LI Yifei, YANG Xiaomiao, WANG Chunping, DUAN Minjie, HUANG Qizhong, HUANG Renzhong, ZHANG Shicai. A New Processing Pepper Cultivar‘Yanjiao 465’ [J]. Acta Horticulturae Sinica, 2024, 51(9): 2223-2224.
[4]	DUAN Minjie, LI Yifei, WANG Chunping, YANG Xiaomiao, HUANG Renzhong, HUANG Qizhong, ZHANG Shicai. Integrated Transcriptomic and Targeted Metabolomic Analysis Reveals Regulation of Carotenoid Accumulation During Pepper Fruit Development [J]. Acta Horticulturae Sinica, 2024, 51(8): 1773-1791.
[5]	SHI Fengyan, WANG Zhidan, ZHANG Xi, WANG Xiuxue, ZOU Chunlei. Research Progress on the Mechanism of Pepper Resistance to Phytophthora Blight [J]. Acta Horticulturae Sinica, 2024, 51(7): 1665-1682.
[6]	ZHANG Ru, CHEN Lingzhi, WANG Lanlan. A New Pepper F₁ Hybrid Cultivar‘Longjiao 13’ [J]. Acta Horticulturae Sinica, 2024, 51(7): 1709-1710.
[7]	GUO Rui, CHEN Gao, ZHAO Huixai, QIAN Yike, LAN Hong, WAN Heping, CHEN Chanyou. A New Purple Pepper Cultivar‘Jiangda Zijiao 1’ [J]. Acta Horticulturae Sinica, 2024, 51(5): 1175-1176.
[8]	FU Wen, ZHU Chenghong, LAN Jiayi, LI Shi, ZHANG Zheng, LIU Feng, DAI Xiongze. Study on the Quality Characteristics of Fresh and Immature Pepper [J]. Acta Horticulturae Sinica, 2024, 51(3): 616-630.
[9]	LUO Xinrui, ZHANG Xiaoxu, WANG Yuping, WANG Zhi, MA Yuanyuan, ZHOU Bingyue. Identification and Expression Analysis of Trihelix Gene Family in Common Bean [J]. Acta Horticulturae Sinica, 2024, 51(12): 2775-2790.
[10]	ZHANG Shaoping, LI Zhou, JU Yudong, LIAN Dongmei, LIN Bizhen, YAO Yunfa, WU Songhai, HONG Jianji, LAI Zhengfeng. Advances in Research on Membrane Lipid Peroxidation Reaction During the Quality Deterioration Period of Fresh Pepper [J]. Acta Horticulturae Sinica, 2024, 51(12): 2945-2961.
[11]	WU Dan, LIU Jiaxin, ZHUO Linxi, LI Yu, LUO Ying, ZHOU Yong, YANG Youxin, YU Ting. Functional Analysis of CaWRKY39 Under Phytophthora capsici Infection in Pepper [J]. Acta Horticulturae Sinica, 2024, 51(10): 2297-2310.
[12]	LUO Yin, LUO Xirong, WU Shiqi, LI Tangyan, LI Jing, QIU Huarong, QIN Cheng. Karyotype Analysis of Four Cultivars of Peppers Based on Fluorescence in situ Hybridization [J]. Acta Horticulturae Sinica, 2024, 51(10): 2311-2319.
[13]	ZOU Xuexiao, YANG Sha, ZHU Fan, YUAN Fang. Progress and Trends in Development of Pepper Industry: High Quality，Good Taste of Fresh Pepper [J]. Acta Horticulturae Sinica, 2024, 51(1): 27-38.
[14]	LIU Shida, QIU Zelan, LI Xiaogang, ZHONG Jie, ZHU Junzi. Identification and Mechanism Characterization of Antagonistic Actinomyces SD-64 Against Pepper Southern Blight [J]. Acta Horticulturae Sinica, 2024, 51(1): 175-189.
[15]	LIAO Fangfang , HU Mingwen, ZHU Wenchao , SU Dan , BAI Liwei , and CHEN Lin. A New Fresh-eating Type Pepper Cultivar‘Layan 201’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 67-68.