Abstract:

The roots and rhizosphere soil of Paeonia ludlowii grown in Nyingchi City，Tibet Autonomous Region（the original site），and Luanchuan County，Henan Province（the introduction site），were used as materials. Based on the high-throughput sequencing technology，this study investigated the differences in the community structure and diversity of the arbuscular mycorrhizal fungi（AMF）in the roots and rhizosphere soil of P. ludlowii from two locations，taking into account the three critical periods of P. ludlowii growth and development - flowering stage，fruit stage and autumn foliage stage. The results showed that the Observed OTU of the AMF community in the roots of P. ludlowii from the original site were higher than those from the introduction site in all three growth periods. The Observed OTU and Chao1 index of the AMF community in the rhizosphere soil of P. ludlowii from the original site were significantly higher than those from the introduction site during the flowering period. However，during the autumn foliage period，the Observed OTU of the AMF community in the rhizosphere soil from the introduction site were significantly higher than those from the original site. The results of principal component analysis showed that the AMF community in the roots of P. ludlowii differed significantly during the flowering period，and the soil AMF community structure differed significantly in all three growth periods，the LEfSe analysis further illustrated that the AMF species in the roots and rhizosphere soil of P. ludlowii differed significantly between the two sites. Rhizophagus，Glomus and Diversispora were the dominant species in the AMF communities in the roots and rhizosphere soil of P. ludlowii from the two sites，but their relative abundance differed significantly between the two sites. Due to the soil environment of the introduction site，the relative abundance of Rhizophagus and Glomus in the AMF community in the roots of P. ludlowii decreased，while the relative abundance of Diversispora and Claroideoglomus increased. Gigaspora was present only in the original site，while Pacispora was exclusively found in the introduction site. The results of Mantel analysis of the soil physicochemical properties and AMF community showed that total nitrogen content was the main factor affecting the roots AMF community of P. ludlowii in the introduction site. Soil’s available potassium content was significantly correlated with the roots AMF community of P. ludlowii in the original site and the soil AMF community in the introduction site. Soil’s available phosphorus content was significantly correlated with the soil AMF community in the original site.

Key words: Paeonia ludlowii, arbuscular mycorrhizal fungi, high-throughout sequencing, community structure