Identification of Virus Disease Types of Melon Planting in Jiashi County of Xinjiang

doi:10.16420/j.issn.0513-353x.2022-0543

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (8): 1793-1802.doi: 10.16420/j.issn.0513-353x.2022-0543

• Plant Protection • Previous Articles Next Articles

Identification of Virus Disease Types of Melon Planting in Jiashi County of Xinjiang

WANG Dong^*(), MAIMAITIAIZEZI · Muhetaer, LIU Yanquan, DILIDAERKEZI · Wulamu

Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region，College of Life and Geographic Sciences，Kashi University，Kashi，Xinjiang 844000，China

Received:2023-02-07 Revised:2023-07-15 Online:2023-08-25 Published:2023-08-23
Contact: WANG Dong

Abstract

Abstract:

In 2019 and 2021,12 towns in Jiashi county of Xinjiang were investigated and sampled for muskmelon（Jiashi-melon）virus disease. Watermelon mosaic virus（WMV），cucumber mosaic virus（CMV），zucchini yellow mosaic virus（ZYMV），cucumber green mottle mosaic virus（CGMMV），cucurbit aphid-borne yellows virus（CABYV），and melon aphid-borne yellows virus（MABYV）were identified by two-step RT-PCR protocol. In addition，the genome of WMV isolates was analyzed. The results showed that the virus disease is more common in the Jiashi-melon planting towns in Jiashi County. Symptoms include mosaic，blister mosaic，blister mosaic and malformation，leaf necrosis，leaf chlorosis，and fruit malformation. A total of 37 samples were collected，and the detection of WMV，CMV，and ZYMV was positive，but CGMMV，CABYV，and MABYV were not detected. 21.6%（8/37）of the samples were positive. WMV was the most prevalent virus detected in 18.9%（7/37）of the 37 samples collected，followed by CMV and ZYMV. Multiple infections were more frequent than single infections. F2/R2（P1 + HC-Pro），F4/R4（P3 + 6K1 + CI），F5/R5（CI + 6K2 + NIa-VPg），F6/R6（6K2 + NIa-VPg + NIa-Pro + NIb），and F8/R8（CP + 3′-UTR）fragments of different WMV isolates infecting Jiashi-melon have certain differences in the branches of the evolutionary tree，indicating that there may be recombination in the WMV genome.

Key words: melon, virus identification, genome analysis, watermelon mosaic virus

WANG Dong, MAIMAITIAIZEZI · Muhetaer, LIU Yanquan, DILIDAERKEZI · Wulamu. Identification of Virus Disease Types of Melon Planting in Jiashi County of Xinjiang[J]. Acta Horticulturae Sinica, 2023, 50(8): 1793-1802.

Figures/Tables 6

References 45

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病毒 Virus	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer	参考文献 Reference
WMV	WMV-F：GGTTGCTGYGARTCAGTGTC	WMV-R：CGACCCGAAATGCTAACT	刘卫荣和向本春，2008
CMV	CMVAu1u：CATGGATGCTTCTCCRCGAG	CMVAu2d：CGTAAGCTGGATGGACAACC	Stanković et al.，2011
ZYMV	CPfwd：GCTCCATACATAGCTGAGAC	Cprev：AACGGAGTCTAATCTCGAGC	Pfosser & Baumann,2002
CGMMV	6F：GATGCATTCTGTTGCAGAGG	6R：TCAACCTCACACACGTAAGAGG	Ali et al.,2004 Shang et al.，2012;吴洋等，2017a
CABYV	Pocon F：TGYTCYGGTTTTGACTGG CA3414F：GGATTTTCTAGCGGTCTA	Pococp R：CGTCTACCTATTTSGGRTTN Pococp R：CGTCTACCTATTTSGGRTTN	Ali et al.,2004 Shang et al.，2012;吴洋等，2017a
MABYV	Pocon F：TGYTCYGGTTTTGACTGG	Pococp R：CGTCTACCTATTTSGGRTTN	Shang et al.，2012;吴洋等，2017a
	MA3669F：CGACGACGACGAAATCCAA	Pococp R;CGTCTACCTATTTSGGRTTN	Shang et al.，2012;吴洋等，2017a

病毒 Virus	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer	参考文献 Reference
WMV	WMV-F：GGTTGCTGYGARTCAGTGTC	WMV-R：CGACCCGAAATGCTAACT	刘卫荣和向本春，2008
CMV	CMVAu1u：CATGGATGCTTCTCCRCGAG	CMVAu2d：CGTAAGCTGGATGGACAACC	Stanković et al.，2011
ZYMV	CPfwd：GCTCCATACATAGCTGAGAC	Cprev：AACGGAGTCTAATCTCGAGC	Pfosser & Baumann,2002
CGMMV	6F：GATGCATTCTGTTGCAGAGG	6R：TCAACCTCACACACGTAAGAGG	Ali et al.,2004 Shang et al.，2012;吴洋等，2017a
CABYV	Pocon F：TGYTCYGGTTTTGACTGG CA3414F：GGATTTTCTAGCGGTCTA	Pococp R：CGTCTACCTATTTSGGRTTN Pococp R：CGTCTACCTATTTSGGRTTN	Ali et al.,2004 Shang et al.，2012;吴洋等，2017a
MABYV	Pocon F：TGYTCYGGTTTTGACTGG	Pococp R：CGTCTACCTATTTSGGRTTN	Shang et al.，2012;吴洋等，2017a
	MA3669F：CGACGACGACGAAATCCAA	Pococp R;CGTCTACCTATTTSGGRTTN	Shang et al.，2012;吴洋等，2017a

引物名称 Name	引物序列（5′-3′） Sequence	扩增片段位置 Position	覆盖的基因组区域 Region
F1/R1	ACACGAATGGCTACAAT/TGTACTCCTCCCAACTAA	127 ~ 1 678	P1 + HC-Pro
F2/R2	TTTGTGGTGCGAGGTAGA/AAGCCTTCCCATCGTCAT	1 383 ~ 2 368	P1 + HC-Pro
F3/R3	CTGAGGGATACAGTAAATACG/AGCCATGAATGCAACAA	2 150 ~ 3 927	HC-Pro + P3 + 6K1
F4/R4	TCCCGTAGCAAAGAGC/GCATATCGGCGTTTGA	3 828 ~ 4 765 4 746 ~ 6 140	P3 + 6K1 + CI
F5/R5	AGGCTCAAACGCCGATAT/CTTCACGCCCTACTTTCC	3 828 ~ 4 765 4 746 ~ 6 140	CI + 6K2 + NIa-VPg
F6/R6	GGATGCTGTGGGACTACTT/TCAATGGCTCAAAGAACT	6 041 ~ 7 558 7 433 ~ 9 012	6K2 + NIa-VPg + NIa-Pro + NIb
F7/R7	ATTTGGTGGCTTGTGGGC/GCCACTGGTGTCCTTCTTCG	6 041 ~ 7 558 7 433 ~ 9 012	NIb + CP
F8/R8	ATTTGGATGCAGGAAAGG/AGGACAACAAACATTACCGT	8 972 ~ 10 046	CP + 3′-UTR

引物名称 Name	引物序列（5′-3′） Sequence	扩增片段位置 Position	覆盖的基因组区域 Region
F1/R1	ACACGAATGGCTACAAT/TGTACTCCTCCCAACTAA	127 ~ 1 678	P1 + HC-Pro
F2/R2	TTTGTGGTGCGAGGTAGA/AAGCCTTCCCATCGTCAT	1 383 ~ 2 368	P1 + HC-Pro
F3/R3	CTGAGGGATACAGTAAATACG/AGCCATGAATGCAACAA	2 150 ~ 3 927	HC-Pro + P3 + 6K1
F4/R4	TCCCGTAGCAAAGAGC/GCATATCGGCGTTTGA	3 828 ~ 4 765 4 746 ~ 6 140	P3 + 6K1 + CI
F5/R5	AGGCTCAAACGCCGATAT/CTTCACGCCCTACTTTCC	3 828 ~ 4 765 4 746 ~ 6 140	CI + 6K2 + NIa-VPg
F6/R6	GGATGCTGTGGGACTACTT/TCAATGGCTCAAAGAACT	6 041 ~ 7 558 7 433 ~ 9 012	6K2 + NIa-VPg + NIa-Pro + NIb
F7/R7	ATTTGGTGGCTTGTGGGC/GCCACTGGTGTCCTTCTTCG	6 041 ~ 7 558 7 433 ~ 9 012	NIb + CP
F8/R8	ATTTGGATGCAGGAAAGG/AGGACAACAAACATTACCGT	8 972 ~ 10 046	CP + 3′-UTR

采用地点 Location	症状表现 Symptom
英买里乡Yingmaili Township	花叶、叶片坏死、叶片褪绿黄化 Mosaic，leaf necrosis，leaf chlorosis
江巴孜乡Gambazi Township	花叶、叶片坏死、叶片褪绿黄化 Mosaic，leaf necrosis，leaf chlorosis
卧里托格拉克镇Wolitogorak Township	花叶、疱斑花叶、叶片坏死、叶片褪绿黄化 Mosaic，blister mosaic，leaf necrosis，leaf chlorosis
克孜勒博依乡Kizilboyi Township	花叶、疱斑花叶、疱斑花叶畸形、叶片坏死、叶片褪绿黄化、果实畸形 Mosaic，blister mosaic，blister mosaic and malformation，leaf necrosis，leaf chlorosis，fruit malformation
米夏乡Mixia Township	花叶、叶片坏死、叶片褪绿黄化 Mosaic，leaf necrosis，leaf chlorosis
夏普吐勒乡Shartuole Township	叶片坏死、叶片褪绿黄化 Leaf necrosis，leaf chlorosis
和夏阿瓦提乡Hexiaavati Township	花叶、叶片褪绿黄化 Mosaic，leaf chlorosis
克孜勒苏乡Kizilsu Township	花叶、疱斑花叶、叶片褪绿黄化 Mosaic，blister mosaic，leaf chlorosis
古勒鲁克乡Guleruk Township	花叶、疱斑花叶、叶片坏死、叶片褪绿黄化 Mosaic，blister mosaic，leaf necrosis，leaf chlorosis
玉代克力克乡Yudaikelik Township	花叶、叶片坏死、叶片褪绿黄化 Mosaic，leaf necrosis，leaf chlorosis
巴仁镇Baren Township	花叶、叶片坏死 Mosaic，leaf necrosis
铁日木乡Tierimu Township	疱斑花叶、叶片褪绿黄化 Blister mosaic，leaf chlorosis

Identification of Virus Disease Types of Melon Planting in Jiashi County of Xinjiang

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采样地点 Location	样品总数 Number of samples	样品中病毒种类Virus species in samples
采样地点 Location	样品总数 Number of samples	WMV	CMV	ZYMV	CGMMV	CABYV	MABYV	WMV+ CMV	WMV+ ZYMV
英买里乡Yingmaili township	3	1	1	0	0	0	0	1	0
江巴孜乡Gambazi township	3	1	0	0	0	0	0	0	0
卧里托格拉克镇Wolitogorak township	9	2	1	1	0	0	0	1	0
克孜勒博依乡Kizilboyi township	3	1	1	0	0	0	0	1	0
米夏乡Mixia township	3	1	0	1	0	0	0	0	1
夏普吐勒乡Shartuole township	2	0	0	0	0	0	0	0	0
和夏阿瓦提乡Hexiaavati township	2	0	0	0	0	0	0	0	0
克孜勒苏乡Kizilsu township	2	0	0	0	0	0	0	0	0
古勒鲁克乡Guleruk township	2	0	0	0	0	0	0	0	0
玉代克力克乡Yudaikelik township	4	1	1	0	0	0	0	1	0
巴仁镇Baren township	2	0	0	0	0	0	0	0	0
铁日木乡Tierimu township	2	0	0	0	0	0	0	0	0
总计Total	37	7	4	2	0	0	0	4	1

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