Research Progress on the Epigenetic Mechanisms of Sex Determination in Horticultural Plants

doi:10.16420/j.issn.0513-353x.2021-0447

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (7): 1602-1610.doi: 10.16420/j.issn.0513-353x.2021-0447

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Research Progress on the Epigenetic Mechanisms of Sex Determination in Horticultural Plants

YANG Linlin¹, HUANG Yuntong², FU Zeyuan¹, XU Qijiang²()

¹Key Laboratory of Northeast Saline-Alkaline Vegetation Restoration and Reconstruction of Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040,China
²School of Laboratory Medicine,Youjiang Medical University for Nationalities,Baise,Guagnxi 533000,China

Received:2021-08-18 Revised:2021-11-03 Online:2022-07-25 Published:2022-07-29

Abstract

Abstract:

The flowers of angiosperm plants are classified into hermaphroditic and unisexual flowers. Some species having both male and female flowers on the same plant（monoecious）,and some species having only one type of unisexual flower per plant（dioecious）. Flowering plants initiate sex differentiation during the transition from vegetative growth to reproductive growth. During sex differentiation of unisexual flowers plants,many studies have demonstrated a variety of mechanisms that determine whether an inflorescence develops as male or female flower. Recently,multiple epigenetic mechanisms,such as DNA methylation,histone modification and microRNA,etc. have been shown function in regulating plant sex determination. In this review,how epigenetic mechanisms—including DNA methylation,histone modification,and diverse microRNAs—function in sex determination of flowers were described in horticultural plants,and they regulates plant sex and floral organ development via plant hormones. At last,the epigenetic mechanism of sex determination was discussed in horticultural crops.

Key words: horticultural plant, unisexual flower, sex determination, epigenetic

CLC Number:

YANG Linlin, HUANG Yuntong, FU Zeyuan, XU Qijiang. Research Progress on the Epigenetic Mechanisms of Sex Determination in Horticultural Plants[J]. Acta Horticulturae Sinica, 2022, 49(7): 1602-1610.

Figures/Tables 1

References 67

[1]	Akagi T, Henry I M, Kawai T, Comai L, Tao R. 2016. Epigenetic regulation of the sex determination gene MeGI in polyploid persimmon. The Plant Cell, 28:2905-2915. doi: 10.1105/tpc.16.00532 URL
[2]	Akagi T, Henry I M, Tao R, Comai L. 2014. Plant genetics. A Y-chromosome-encoded small RNA acts as a sex determinant in persimmons. Science, 346:646-650. doi: 10.1126/science.1257225 URL
[3]	Alonso-Peral M M, Li J, Li Y, Allen R S, Schnippenkoetter W, Ohms S, White R G, Millar A A. 2010. The microRNA159-regulated GAMYB-like genes inhibit growth and promote programmed cell death in Arabidopsis. Plant Physiol, 154:757-771. doi: 10.1104/pp.110.160630 pmid: 20699403
[4]	Aryal R, Jagadeeswaran G, Zheng Y, Yu Q, Sunkar R, Ming R. 2014. Sex specific expression and distribution of small RNAs in papaya. BMC Genomics, 15:20. doi: 10.1186/1471-2164-15-20 URL
[5]	Aukerman M J, Sakai H. 2003. Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes. Plant Cell, 15:2730-2741. pmid: 14555699
[6]	Aya K, Hiwatashi Y, Kojima M, Sakakibara H, Ueguchi-Tanaka M, Hasebe M, Matsuoka M. 2011. The gibberellin perception system evolved to regulate a pre-existing GAMYB-mediated system during land plant evolution. Nat Commun, 2:544. doi: 10.1038/ncomms1552 URL
[7]	Aya K, Ueguchi-Tanaka M, Kondo M, Hamada K, Yano K, Nishimura M, Matsuoka M. 2009. Gibberellin modulates anther development in rice via the transcriptional regulation of GAMYB. Plant Cell, 21:1453-1472. doi: 10.1105/tpc.108.062935 URL
[8]	Bacovsky V, Houben A, Kumke K, Hobza R. 2019. The distribution of epigenetic histone marks differs between the X and Y chromosomes in Silene latifolia. Planta, 250:487-494. doi: 10.1007/s00425-019-03182-7
[9]	Banks J A. 2008. MicroRNA,sex determination and floral meristem determinacy in maize. Genome Biol, 9:204. doi: 10.1186/gb-2008-9-1-204 pmid: 18254926
[10]	Bannister A J, Kouzarides T. 2011. Regulation of chromatin by histone modifications. Cell Res, 21:381-395. doi: 10.1038/cr.2011.22 pmid: 21321607
[11]	Casanova-Saez R, Voss U. 2019. Auxin metabolism controlsdevelopmental decisions in land plants. Trends Plant Sci, 24:741-754. doi: S1360-1385(19)30124-4 pmid: 31230894
[12]	Chen J, Zheng Y, Qin L, Wang Y, Chen L, He Y, Fei Z, Lu G. 2016. Identification of miRNAs and their targets through high-throughput sequencing and degradome analysis in male and female Asparagus officinalis. BMC Plant Biol, 16:80. doi: 10.1186/s12870-016-0770-z URL
[13]	Consuegra S, Rodriguez Lopez C M. 2016. Epigenetic-induced alterations in sex-ratios in response to climate change:an epigenetic trap? Bioessays, 38:950-958. doi: 10.1002/bies.201600058 pmid: 27548838
[14]	Das A, Saxena S, Kumar K, Tribhuvan K U, Singh N K, Gaikwad K. 2020. Non-coding RNAs having strong positive interaction with mRNAs reveal their regulatory nature during flowering in a wild relative of pigeonpea(Cajanus scarabaeoides). Mol Biol Rep, 47:3305-3317. doi: 10.1007/s11033-020-05400-y URL
[15]	Diggle P K, Di Stilio V S, Gschwend A R, Golenberg E M, Moore R C, Russell J R, Sinclair J P. 2011. Multiple developmental processes underlie sex differentiation in angiosperms. Trends Genet, 27:368-376. doi: 10.1016/j.tig.2011.05.003 URL
[16]	Ding J, Lu Q, Ouyang Y, Mao H, Zhang P, Yao J, Xu C, Li X, Xiao J, Zhang Q. 2012. A long noncoding RNA regulates photoperiod-sensitive male sterility,an essential component of hybrid rice. Proc Natl Acad Sci U S A, 109:2654-2659. doi: 10.1073/pnas.1121374109 URL
[17]	Du Z, Li H, Wei Q, Zhao X, Wang C, Zhu Q, Yi X, Xu W, Liu X S, Jin W, Su Z. 2013. Genome-wide analysis of histone modifications:H3K4me2,H3K4me3,H3K9ac,and H3K27ac in Oryza sativa L. japonica. Mol Plant, 6:1463-1472.
[18]	Emerson R A, Emerson S H. 1922. Genetic interrelations of two andromonoecious types of maize,dwarf and anther ear. Genetics, 7:203-236. doi: 10.1093/genetics/7.3.203 pmid: 17245980
[19]	Gao Ming, Cheng Yi-cun, Yang Su-su, Liu Ying-guan, Zhu Hui-ping, Wang Yang-dong. 2015. Progress on sex differentiation in unisexual flower plants. Acta Prataculturae Sinica, 24 (11):206-217. (in Chinese)
	高暝, 陈益存, 杨素素, 刘英冠, 朱慧萍, 汪阳东. 2015. 单性花植物性别分化研究进展. 草业学报, 24 (11):206-217.
[20]	Ge W, Zhang Y, Cheng Z, Hou D, Li X, Gao J. 2017. Main regulatory pathways,key genes and microRNAs involved in flower formation and development of Moso bamboo(Phyllostachys edulis). Plant Biotechnol J, 15:82-96. doi: 10.1111/pbi.12593 URL
[21]	Golicz A A, Bhalla P L, Singh M B. 2018. LncRNAs in plant and animal sexual reproduction. Trends Plant Sci, 23:195-205. doi: 10.1016/j.tplants.2017.12.009 URL
[22]	Hollister J D, Gaut B S. 2009. Epigenetic silencing of transposable elements:a trade-off between reduced transposition and deleterious effects on neighboring gene expression. Genome Res, 19:1419-1428. doi: 10.1101/gr.091678.109 pmid: 19478138
[23]	Jung J H, Seo Y H, Seo P J, Reyes J L, Yun J, Chua N H, Park C M. 2007. The GIGANTEA-regulated microRNA172 mediates photoperiodic flowering independent of CONSTANS in Arabidopsis. Plant Cell, 19:2736-2748. doi: 10.1105/tpc.107.054528 URL
[24]	Lai Y S, Zhang X, Zhang W, Shen D, Wang H, Xia Y, Qiu Y, Song J, Wang C, Li X. 2017. The association of changes in DNA methylation with temperature-dependent sex determination in cucumber. J Exp Bot, 68:2899-2912. doi: 10.1093/jxb/erx144 URL
[25]	Latrasse D, Rodríguez-Granados N Y, Veluchamy A, Mariappan K G, Bevilacqua C, Crapart N, Camps C, Sommard V, Raynaud C, Dogimont C, Boualem A, Benhamed M, Bendahmane A. 2017. The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo. Epigenetics Chromatin, 10:22. doi: 10.1186/s13072-017-0132-6 pmid: 28592995
[26]	Li N, Meng Z, Tao M, Wang Y, Zhang Y, Li S, Gao W, Deng C. 2020. Comparative transcriptome analysis of male and female flowers in Spinacia oleracea L. BMC Genomics, 21:850. doi: 10.1186/s12864-020-07277-4 URL
[27]	Li S F, Zhang G J, Yuan J H, Deng C L, Gao W J. 2016. Repetitive sequences and epigenetic modification:inseparable partners play important roles in the evolution of plant sex chromosomes. Planta, 243:1083-1095. doi: 10.1007/s00425-016-2485-7 URL
[28]	Li X Y, Guo F, Ma S Y, Zhu M Y, Pan W H, Bian H W. 2019. Regulation of flowering time via miR172-mediated APETALA2-like expression in ornamental gloxinia(Sinningia speciosa). J Zhejiang Univ Sci B, 20:322-331. doi: 10.1631/jzus.B1800003 URL
[29]	Li Ying, Meng Xianwei, Ma Zhihang, Liu Mengjun, Zhao Jin. 2022. Identification and expression analysis of microRNA families associated with phase transition in Chinese jujube. Acta Horticulturae Sinica, 49 (1):23-40. (in Chinese)
	李莹, 孟宪巍, 马志航, 刘孟军, 赵锦. 2022. 枣树阶段转变相关microRNA家族的鉴定及其表达分析. 园艺学报, 49 (1):23-40.
[30]	Li Z F, Zhang Y C, Chen Y Q. 2015. MiRNAs and lncRNAs in reproductive development. Plant Sci, 238:46-52. doi: 10.1016/j.plantsci.2015.05.017 URL
[31]	Liu H, Wang R, Mao B, Zhao B, Wang J. 2019. Identification of lncRNAs involved in rice ovule development and female gametophyte abortion by genome-wide screening and functional analysis. BMC Genomics, 20:90. doi: 10.1186/s12864-019-5442-6 URL
[32]	Liu J, Chatham L, Aryal R, Yu Q, Ming R. 2018. Differential methylation and expression of HUA1 ortholog in three sex types of papaya. Plant Sci, 272:99-106. doi: 10.1016/j.plantsci.2018.04.001 URL
[33]	Ma J, Yan B, Qu Y, Qin F, Yang Y, Hao X, Yu J, Zhao Q, Zhu D, Ao G. 2008. Zm401,a short-open reading-frame mRNA or noncoding RNA, is essential for tapetum and microspore development and can regulate the floret formation in maize. J Cell Biochem, 105:136-146. doi: 10.1002/jcb.21807 URL
[34]	Marciniak K, Przedniczek K. 2021. Anther dehiscence is regulated by gibberellic acid in yellow lupine(Lupinus luteus L.). BMC Plant Biol, 21:314. doi: 10.1186/s12870-021-03085-4 URL
[35]	Martin A, Troadec C, Boualem A, Rajab M, Fernandez R, Morin H, Pitrat M, Dogimont C, Bendahmane A. 2009. A transposon-induced epigenetic change leads to sex determination in melon. Nature, 461:1135-1138. doi: 10.1038/nature08498 URL
[36]	Mateo-Bonmatí E, Casanova-Sáez R, Ljung K. 2019. Epigenetic regulation of auxin homeostasis. Biomolecules, 9 (10):623. doi: 10.3390/biom9100623 URL
[37]	Millar A A, Lohe A, Wong G. 2019. Biology and function of miR159 in plants. Plants(Basel), 8 (8):255.
[38]	Murase K, Shigenobu S, Fujii S, Ueda K, Murata T, Sakamoto A, Wada Y, Yamaguchi K, Osakabe Y, Osakabe K, Kanno A, Ozaki Y, Takayama S. 2017. MYB transcription factor gene involved in sex determination in Asparagus officinalis. Genes Cells, 22:115-123. doi: 10.1111/gtc.12453 pmid: 27869347
[39]	Pan J, Wang G, Wen H, Du H, Lian H, He H, Pan J, Cai R. 2018. Differential gene expression caused by the F and M loci provides insight into ethylene-mediated female flower differentiation in cucumber. Front Plant Sci, 9:1091. doi: 10.3389/fpls.2018.01091 URL
[40]	Piferrer F. 2013. Epigenetics of sex determination and gonadogenesis. Dev Dyn, 242:360-370. doi: 10.1002/dvdy.23924 URL
[41]	Qin Li. 2016. Comparative transcriptome analysis of male and female flowers and identification of gender-related miRNA targets in Asparagus officinalis L [M. S. Dissertation]. Hangzhou:Zhejiang University. (in Chinese)
	秦力. 2016. 芦笋雌雄花发育转录组分析及性别决定相关miRNA靶基因的鉴定[硕士论文]. 杭州: 浙江大学.
[42]	Rodríguez Lorenzo J L, Hobza R, Vyskot B. 2018. DNA methylation and genetic degeneration of the Y chromosome in the dioecious plant Silene latifolia. BMC Genomics, 19:540. doi: 10.1186/s12864-018-4936-y pmid: 30012097
[43]	Rodríguez Lorenzo J L, Hubinsky M, Vyskot B, Hobza R. 2020. Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system. Plant Sci, 299:110528. doi: 10.1016/j.plantsci.2020.110528 URL
[44]	Shivaraj S M, Jain A, Singh A. 2018. Highly preserved roles of Brassica mIR172 in polyploid Brassicas:ectopic expression of variants of Brassica mIR172 accelerates floral transition. Mol Genet Genomics, 293:1121-1138. doi: 10.1007/s00438-018-1444-3 pmid: 29752548
[45]	Smith A R, Zhao D. 2016. Sterility Caused by Floral Organ degeneration and abiotic stresses in Arabidopsis and cereal grains. Front Plant Sci, 7:1503.
[46]	Somyong S, Poopear S, Sunner S K, Wanlayaporn K, Jomchai N, Yoocha T, Ukoskit K, Tangphatsornruang S, Tragoonrung S. 2016. ACC oxidase and miRNA159a, and their involvement in fresh fruit bunch yield (FFB) via sex ratio determination in oil palm. Mol Genet Genomics, 291:1243-1257. doi: 10.1007/s00438-016-1181-4 URL
[47]	Song J H, Cao J S, Wang C G. 2013. BcMF11, a novel non-coding RNA gene from Brassica campestris,is required for pollen development and male fertility. Plant Cell Rep, 32:21-30. doi: 10.1007/s00299-012-1337-6 URL
[48]	Song J H, Cao J S, Yu X L, Xiang X. 2007. BcMF11,a putative pollen-specific non-coding RNA from Brassica campestris ssp.chinensis. J Plant Physiol, 164:1097-1100. doi: 10.1016/j.jplph.2006.10.002 URL
[49]	Tsuji H, Aya K, Ueguchi-Tanaka M, Shimada Y, Nakazono M, Watanabe R, Nishizawa N K, Gomi K, Shimada A, Kitano H, Ashikari M, Matsuoka M. 2006. GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. Plant J, 47:427-444. doi: 10.1111/j.1365-313X.2006.02795.x URL
[50]	Vyskot B, Araya A, Veuskens J, Negrutiu I, Mouras A. 1993. DNA methylation of sex chromosomes in a dioecious plant,Melandrium album. Mol Gen Genet, 239:219-224. doi: 10.1007/BF00281621 URL
[51]	Wang B, Fang R, Chen F, Han J, Liu Y G, Chen L, Zhu Q. 2020. A novel CCCH-type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice. J Integr Plant Biol, 62:1594-1606. doi: 10.1111/jipb.12924 URL
[52]	Wang L, Han W, Diao S, Suo Y, Li H, Mai Y, Wang Y, Sun P, Fu J. 2021. Study of sexual-linked genes(OGI and MeGI) on the performance of androecious persimmons(Diospyros kaki Thunb.). Plants(Basel), 10. DOI: 10.3390/plants10020390. doi: 10.3390/plants10020390 URL
[53]	Wang T, Ping X, Cao Y, Jian H, Gao Y, Wang J, Tan Y, Xu X, Lu K, Li J, Liu L. 2019. Genome-wide exploration and characterization of miR172/euAP2 genes in Brassica napus L. for likely role in flower organ development. BMC Plant Biol, 19:336. doi: 10.1186/s12870-019-1936-2 URL
[54]	Wang You-ning, Su Chao, Zou Yan-min, Wang Li-xiang, Li Xia. 2018. Research progress of microRNA172 in plant development and stress responses. Chinese Bulletin of Life Sciences, 28:645-654. (in Chinese)
	王幼宁, 苏超, 邹艳敏, 王利祥, 李霞. 2016. microRNA172参与植物生长发育及逆境响应的研究进展. 生命科学, 28:645-654.
[55]	Xu Z L, Chen Y C, Gao M, Wu L W, Zhao Y X, Wang Y D. 2019. Research progress in sex differentiation in angiosperms. Scientia Silvae Sinicae, 55:157-169.
[56]	Xie Y, Chen L. 2020. Epigenetic regulation of Gibberellin metabolism and signaling. Plant Cell Physiol, 61:1912-1918. doi: 10.1093/pcp/pcaa101 URL
[57]	Yamamuro C, Zhu J K, Yang Z. 2016. Epigenetic modifications and plant hormone action. Mol Plant, 9:57-70. doi: S1674-2052(15)00403-7 pmid: 26520015
[58]	Yang H, Chang F, You C, Cui J, Zhu G, Wang L, Zheng Y, Qi J, Ma H. 2015. Whole-genome DNA methylation patterns and complex associations with gene structure and expression during flower development in Arabidopsis. The Plant Journal, 81:268-281. doi: 10.1111/tpj.12726 URL
[59]	Yu S, Wang J W. 2020. The crosstalk between microRNAs and gibberellin signaling in plants. Plant Cell Physiol, 61:1880-1890. doi: 10.1093/pcp/pcaa079 URL
[60]	Zhang H, Lang Z, Zhu J K. 2018. Dynamics and function of DNA methylation in plants. Nat Rev Mol Cell Biol, 19:489-506. doi: 10.1038/s41580-018-0016-z URL
[61]	Zhang W, Garcia N, Feng Y, Zhao H, Messing J. 2015. Genome-wide histone acetylation correlates with active transcription in maize. Genomics, 106:214-220. doi: 10.1016/j.ygeno.2015.05.005 pmid: 26021446
[62]	Zhang W, Wang X, Yu Q, Ming R, Jiang J. 2008. DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya. Genome Res, 18:1938-1943. doi: 10.1101/gr.078808.108 URL
[63]	Zhang X, Yazaki J, Sundaresan A, Cokus S, Chan S W, Chen H, Henderson I R, Shinn P, Pellegrini M, Jacobsen S E, Ecker J R. 2006. Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell, 126:1189-1201. doi: 10.1016/j.cell.2006.08.003 URL
[64]	Zhang Y, Zhang X, Liu B, Wang W, Liu X, Chen C, Liu X, Yang S, Ren H. 2014. A GAMYB homologue CsGAMYB1 regulates sex expression of cucumber via an ethylene-independent pathway. J Exp Bot, 65:3201-3213. doi: 10.1093/jxb/eru176 pmid: 24790111
[65]	Zhao Y, Wang S, Wu W, Li L, Jiang T, Zheng B. 2018. Clearance of maternal barriers by paternal miR159 to initiate endosperm nuclear division in Arabidopsis. Nat Commun, 9:5011. doi: 10.1038/s41467-018-07429-x URL
[66]	Zhou Chenping, Yang Min, Guo Jinju, Kuang Ruibin, Yang Hu, Huang Bingxiong, Wei Yuerong. 2022. Dynamic changes in DNA methylome and transcriptome patterns during papaya fruit ripening. Acta Horticulturae Sinica, 49 (3):519-532. (in Chinese)
	周陈平, 杨敏, 郭金菊, 邝瑞彬, 杨护, 黄炳雄, 魏岳荣. 2022. 番木瓜成熟过程中全基因组DNA甲基化和转录组变化分析. 园艺学报, 49 (3):519-532.
[67]	Zhou J, Wang X, He K, Charron J B, Elling A A, Deng X W. 2010. Genome-wide profiling of histone H3 lysine 9 acetylation and dimethylation in Arabidopsis reveals correlation between multiple histone marks and gene expression. Plant Mol Biol, 72:585-595. doi: 10.1007/s11103-009-9594-7 URL

Research Progress on the Epigenetic Mechanisms of Sex Determination in Horticultural Plants

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