Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (4): 765-777.doi: 10.16420/j.issn.0513-353x.2022-0094
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WANG Ping, SHENG Ling, YANG Jinpeng, ZHOU Linglei, JIN Yan, LUO Xuzhao, MA Xianfeng, DENG Ziniu()
Received:
2022-05-02
Revised:
2022-10-15
Online:
2023-04-25
Published:
2023-04-27
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*(E-mail:deng7009@163.com)
E-mail:deng7009@163.com
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WANG Ping, SHENG Ling, YANG Jinpeng, ZHOU Linglei, JIN Yan, LUO Xuzhao, MA Xianfeng, DENG Ziniu. Evaluation of Resistance to Citrus Canker Disease in Hybrid Progeny of Red Pomelo and American Citron[J]. Acta Horticulturae Sinica, 2023, 50(4): 765-777.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2022-0094
Fig. 3 Symptoms at 20 d after injection of single live leaf of F1 part of Red pomelo and American citron (105 and 104 cfu · mL-1)with ulcerative pathogen
Fig. 4 Symptoms of single isolated leaves of hybrid F2 of Red pomelo and American citron inoculated with ulcerative pathogen(105,106 and 107 cfu · mL-1)at 10 d
F1 单株 F1 plant | 植株数 Number of plants | ||||||
---|---|---|---|---|---|---|---|
F2 | 等级1 Grade Ⅰ | 等级2 Grade Ⅱ | 等级3 Grade Ⅲ | 等级4 Grade Ⅳ | 叶正面极感 Adaxial leaf surface sensation | 叶反面极感 Abaxial leaf surface serious | |
E48 | 156 | 3 | 5 | 70 | 46 | 5 | 9 |
E73 | 63 | 1 | 1 | 31 | 23 | 1 | 1 |
E65 | 16 | — | 22 | 7 | — | 4 | — |
E5 | 18 | — | 2 | 10 | 3 | — | 1 |
总计(株) Total(plant) | 253 | 4 | 10 | 118 | 72 | 10 | 11 |
Table 1 Classification of canker disease sumptoms following in vivo inoculation of F2 plants of Red pumelo × American citron
F1 单株 F1 plant | 植株数 Number of plants | ||||||
---|---|---|---|---|---|---|---|
F2 | 等级1 Grade Ⅰ | 等级2 Grade Ⅱ | 等级3 Grade Ⅲ | 等级4 Grade Ⅳ | 叶正面极感 Adaxial leaf surface sensation | 叶反面极感 Abaxial leaf surface serious | |
E48 | 156 | 3 | 5 | 70 | 46 | 5 | 9 |
E73 | 63 | 1 | 1 | 31 | 23 | 1 | 1 |
E65 | 16 | — | 22 | 7 | — | 4 | — |
E5 | 18 | — | 2 | 10 | 3 | — | 1 |
总计(株) Total(plant) | 253 | 4 | 10 | 118 | 72 | 10 | 11 |
Fig. 5 Symptoms(20 d)of single living leaves of the F2 part of a hybrid of Red pumelo × American citron inoculated with ulcerative bacteria(105 and 104 cfu · mL-1)
Fig. 6 Symptoms of large difference between the front and back sides of the leaves(20 d)of a single plant of F2 hybridization of Red pumelo × American citron inoculated with ulcerative bacteria(105 and 104 cfu · mL-1)
Fig. 7 Growth of ulcerative bacteria after inoculation with different resistant germplasm and some F2 plant leaves BTC:‘Bingtang’sweet orange;HXY:Red pommelo;JYC05:Citron C-05;MGJY:American citron. Different lowercase letters indicate signicant differences in the content of ulcerative bacteria between different leaves(P < 0.05).
F1单株 F1 plant | 植株数 Number of plants | |||
---|---|---|---|---|
F2 | 活体鉴定 Identified | 抗病株 Resistant plant | 感病株Susceptible plant | |
E48 | 156 | 138 | 8 | 130 |
E73 | 63 | 58 | 2 | 56 |
E65 | 16 | 13 | 2 | 11 |
E5 | 18 | 16 | 2 | 14 |
总计Total | 253 | 225 | 14 | 211 |
Table 2 Summary of the resistant and susceptle F2 plants of Red pomelo × American citron
F1单株 F1 plant | 植株数 Number of plants | |||
---|---|---|---|---|
F2 | 活体鉴定 Identified | 抗病株 Resistant plant | 感病株Susceptible plant | |
E48 | 156 | 138 | 8 | 130 |
E73 | 63 | 58 | 2 | 56 |
E65 | 16 | 13 | 2 | 11 |
E5 | 18 | 16 | 2 | 14 |
总计Total | 253 | 225 | 14 | 211 |
[1] | Amaral A M, Carvalho S A, Silva L F C. 2010. Reaction of genotypes of citrus species and varieties to Xanthomonas citri subsp. citri under greenhouse conditions. Journal of Plant Pathology, 92:519-524. |
[2] |
Brunings A M, Gabriel D W. 2003. Xanthomonas citri:breaking the surface. Mol Plant Pathol, 4 (3):141-157.
doi: 10.1046/j.1364-3703.2003.00163.x pmid: 20569374 |
[3] | Chen Li-geng. 1993. Inheritance and breeding of resistance to citrus canker disease. China Citrus,(1):19-20. (in Chinese) |
陈力耕. 1993. 柑桔溃疡病的抗性遗传与育种. 中国柑桔,(1):19-20. | |
[4] |
Deng Z N, Xu L, Li D Z, Long G Y, Liu L P, Fang F, Shu G P. 2010. Screening citrus genotypes for resistance to canker disease(Xanthomonas axonopodis citri). Plant Breeding, 129:341-345.
doi: 10.1111/pbr.2010.129.issue-3 URL |
[5] |
Ge Hongjuan, Long Guiyou, Dai Suming, Li Dazhi, Li Na, Deng Ziniu. 2015. Effects of sugar orange and citron C-05 on growth characteristics of Ulcerative bacteria. Scientia Agricultura Sinica, 48 (7):1383-1391. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2015.07.13 |
葛红娟, 龙桂友, 戴素明, 李大志, 李娜, 邓子牛. 2015. 冰糖橙与枸橼C-05对溃疡病菌生长特性的影响. 中国农业科学, 48 (7):1383-1391.
doi: 10.3864/j.issn.0578-1752.2015.07.13 |
|
[6] |
Gottwald T R. 1993. Differential host range reaction of citrus and citrus relatives to citrus canker and citrus bacterial spot determinated by leaf mesophyll susceptibility. Plant Dis, 77 (10):1004-1009.
doi: 10.1094/PD-77-1004 URL |
[7] | Hu Qing-bo. 2010. Evaluation on resistance of citrus germplasm resources to canker disease[M. D. Dissertation]. Changsha: Hunan Agricultural University. (in Chinese) |
胡青波. 2010. 柑橘种质资源抗溃疡病评价[硕士论文]. 长沙: 湖南农业大学. | |
[8] | Hu Zhe. 2018. Genetic analysis of leaf traits and resistance to canker disease in citron and its hybrids[M. D. Dissertation]. Changsha: Hunan Agricultural University. (in Chinese) |
胡哲. 2018. 枸橼及其杂种叶性状和对溃疡病抗性的遗传学分析[硕士论文]. 长沙: 湖南农业大学. | |
[9] | Li Dazhi, Deng Ziniu, Gan Lin, Xiong Xingyao, Zhang Qiuming. 2005. Advances in studies on citrus canker. Journal of Hunan Agricultural University(Natural Science Edition), 31 (4):105-108. (in Chinese) |
李大志, 邓子牛, 甘霖, 熊兴耀, 张秋明. 2005. 柑橘溃疡病研究进展. 湖南农业大学学报(自然科学版), 31 (4):105-108. | |
[10] | Li Jianhui. 2013. Study on effective control of citrus canker disease[M. D. Dissertation]. Changsha: Hunan Agricultural University. (in Chinese) |
李建挥. 2013. 柑橘溃疡病有效防治的研究[硕士论文]. 长沙: 湖南农业大学. | |
[11] | Liu Liping. 2013. Study on pathogenic factors of citrus ulcer pathogen[Ph. D. Dissertation]. Changsha: Hunan Agricultural University. (in Chinese) |
刘利平. 2013. 柑橘溃疡病菌致病性影响因素的研究[博士论文]. 长沙: 湖南农业大学. | |
[12] |
Ma Mingying, Hao Chenxing, Zhang Kai, Xiao Guihua, Su Hanying, Wen Kang, Deng Ziniu, Ma Xianfeng. 2022. CsSWEET2a promotes the infection of Xanthomonas citri subsp. citri. Acta Horticulturae Sinica, 49 (6):1247-1260. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2021-0323 |
麻明英, 郝晨星, 张凯, 肖桂华, 苏翰英, 文康, 邓子牛, 马先锋. 2022. 甜橙SWEET2a促进柑橘溃疡病菌侵染. 园艺学报, 49 (6):1247-1260.
doi: 10.16420/j.issn.0513-353x.2021-0323 URL |
|
[13] |
Ma Zhimin, Duan Yu, Xu Jianjian, Bin Yu, Zhou Changyong, Song Zhen. 2021. The rapid detection of Xanthomonas citri ssp. citri(Xcc)based on recombinase polymerase amplification(RPA)assay. Acta Horticulturae Sinica, 48 (3):590-599. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2020-0545 URL |
马志敏, 段玉, 许建建, 宾羽, 周常勇, 宋震. 2021. 基于重组酶聚合酶扩增技术(RPA)的柑橘溃疡病菌检测方法. 园艺学报, 48 (3):590-599.
doi: 10.16420/j.issn.0513-353x.2020-0545 URL |
|
[14] |
Nicolosi E, Deng Z N, Gentile A. 2000. Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor Appl Genet, 100:1155-1166.
doi: 10.1007/s001220051419 URL |
[15] | Peng Zhuchun, Gong Guizhi, Chen Shanchun, Zhang Gebi, Hong Qibin. 2010. Screening of SSR markers related to resistance to citrus canker disease. Acta horticulturae sinica, 37 (3):383-389. (in Chinese) |
彭祝春, 龚桂芝, 陈善春, 张戈壁, 洪棋斌. 2010. 柑橘溃疡病抗性相关的SSR标记筛选. 园艺学报, 37 (3):383-389. | |
[16] |
Schaad N W. 2006. Emended classification of xanthomonad pathogens on citrus. Syst Appl Microbiol, 29:690-695.
pmid: 17183629 |
[17] |
Shi Q C. 2015. Responsiveness of different citrus genotypes to the Xanthomonas citri ssp. citri-derived pathogen-associated molecular pattern (PAMP)flg 22 correlates with resistance to citrus canker. Mol Plant Pathol, 16 (5):507-520.
doi: 10.1111/mpp.2015.16.issue-5 URL |
[18] | Song Shengying, Yang Guanglan, Pan Shengyong, Zhao Chunmu, Shi Anjiu. 2002. Investigation on resistance of different citrus cultivars to citrus canker disease. Journal of Forest Diseases and Pests in China, 21 (2):34-36. (in Chinese) |
宋盛英, 杨光兰, 潘盛勇, 赵春穆, 石安鸠. 2002. 不同柑橘品种对柑橘溃疡病的抗性调查. 中国森林病虫, 21 (2):34-36. | |
[19] | Tan Li-mei. 2019. Evaluation of resistance of lemons and their selfing progeny to citrus canker disease[M. D. Dissertation]. Changsha: Hunan Agricultural University. (in Chinese) |
谭李梅. 2019. 柠檬及其自交后代对柑橘溃疡病的抗性评价[硕士论文]. 长沙: 湖南农业大学. | |
[20] | Tan Limei, Fu Hongyan, Xu Jing, Zhu Zhimei, Hu Zhe, Xiao Shunyuan, Ma Xianfeng, Deng Ziniu. 2020. Identification of selfing seedlings of lemon zygote embryos and preliminary evaluation of resistance to Citrus canker disease. Molecular Plant Breeding, 18 (7):2258-2267. (in Chinese) |
谭李梅, 符红艳, 徐静, 朱志媚, 胡哲, 肖顺元, 马先锋, 邓子牛. 2020. 柠檬合子胚自交实生苗的鉴定及其对柑橘溃疡病的抗性初步评价. 分子植物育种, 18 (7):2258-2267. | |
[21] | Tan Limei, Liu Hui, Zhu Zhimei, Zhou Dong, Tang Tian, Deng Ziniu. 2017. Lemon selfing generations in vitro identification of citrus canker resistant. Journal of Hunan Agricultural Science,(3):58-62. (in Chinese) |
谭李梅, 刘慧, 朱志媚, 周东, 汤甜, 邓子牛. 2017. 柠檬自交后代抗柑橘溃疡病的离体鉴定. 湖南农业科学,(3):58-62. | |
[22] |
Viloria Z, Drouillard D L, Graham J H, Grosser J W. 2004. Screening triploid hybrids of‘Lakeland’Limequat for resistance to citrus canker. Plant Dis, 88 (10):1056-1060.
doi: 10.1094/PDIS.2004.88.10.1056 pmid: 30795244 |
[23] | Xu Jing. 2020. Genetic identification of citron germplasm based on resistance to citrus canker disease[M. D. Dissertation]. Changsha: Hunan Agricultural University. (in Chinese) |
徐静. 2020. 基于柑橘溃疡病抗性的构橼种质亲缘关系鉴定[硕士论文]. 长沙: 湖南农业大学. | |
[24] | Xu Jing, Tan Limei, Fu Hongyan, Zhu Zhimei, Long Libing, Hu Zhe, Ma Xianfeng, Deng Ziniu. 2021. Diversity analysis of 14 citron germplasm using molecular markers. Molecular Plant Breeding, 19 (14):4726-4737.. (in Chinese) |
徐静, 谭李梅, 符红艳, 朱志媚, 龙栎冰, 胡哲, 马先锋, 邓子牛. 2021. 利用分子标记对14份枸橼种质进行多样性分析. 分子植物育种, 19 (14):4726-4737. | |
[25] | Zhang Gebi, Yang Tingmi, Li Xiqing, Chen Zhusheng. 2004. Determination of resistance of different citrus varieties to citrus canker disease. Chinese Journal of Plant Protection, 31 (2):221-222. (in Chinese) |
张戈壁, 阳廷密, 李喜庆, 陈竹生. 2004. 不同柑桔品种对柑桔溃疡病抗病能力的测定. 植物保护学报, 31 (2):221-222. | |
[26] | Zhang Kai, Ma Mingying, Wang Ping, Li Yi, Jin Yan, Sheng Ling, Deng Ziniu, Ma Xianfeng. 2022. Identification of HSP 20 family genes in citrus and their expression in pathogen infection responses citrus canker. Acta Horticulturae Sinica, 49 (6):1213-1232. (in Chinese) |
张凯, 麻明英, 王萍, 李益, 金燕, 盛玲, 邓子牛, 马先锋. 2022. 柑橘HSP20家族基因鉴定及其响应溃疡病菌侵染表达分析. 园艺学报, 49 (6):1213-1232.
doi: 10.16420/j.issn.0513-353x.2021-0331 URL |
|
[27] | Zhu Zhimei, Tan Limei, Xu Jing, Fu Hongyan, Hu Zhe, Gong Lei, Yang Guibing, Wang Ping, Ma Xianfeng, Deng Ziniu. 2021. Identification of resistance of citron germplasm to Citrus canker disease. China Fruits,(1):43- 49,110-111. (in Chinese) |
朱志媚, 谭李梅, 徐静, 符红艳, 胡哲, 龚蕾, 杨贵兵, 王萍, 马先锋, 邓子牛. 2021. 枸橼种质对柑橘溃疡病的抗性鉴定. 中国果树,(1):43- 49,110-111. |
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