Evaluation of Resistance to Citrus Canker Disease in Hybrid Progeny of Red Pomelo and American Citron

doi:10.16420/j.issn.0513-353x.2022-0094

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (4): 765-777.doi: 10.16420/j.issn.0513-353x.2022-0094

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Evaluation of Resistance to Citrus Canker Disease in Hybrid Progeny of Red Pomelo and American Citron

WANG Ping, SHENG Ling, YANG Jinpeng, ZHOU Linglei, JIN Yan, LUO Xuzhao, MA Xianfeng, DENG Ziniu()

Changsha Subcenter，National Center for Citrus Improvement，College of Horticulture，Hunan Agricultural University，Changsha 410128，China

Received:2022-05-02 Revised:2022-10-15 Online:2023-04-25 Published:2023-04-27
Contact: *（E-mail：deng7009@163.com） E-mail:deng7009@163.com

Abstract

Abstract:

In order to investigate the genetic mechanism of citron in resistance against citrus canker disease（Xanthomonas citri subsp. citri，Xcc），in this study，the susceptible variety Red pomelo was crossed with the resistant genotype American citron and F₁ hybrids were obtained. F₂ progeny was further gained by self-crossing the F₁ plants. Resistance identification wan performed on the F₁ hybrid plants by in vivo inoculation of Xcc. All the F₂ plants were in vitro inoculated and 225 F₂ plants were successively identified for the resistance by in vivo inoculation. Bacterial quantification was conducted on some of the F₂plants following the inoculation with Xcc. The results from in vivo inoculation of F₁ plants indicated that all F₁ hybrids were susceptible，and no segregation between resistance and susceptibility was observed on the F₁ progeny. In vitro identification of 253 F₂ plants showed that 76 plants were resistant and 177 were susceptible. The results of in vivo inoculation on 225 F₂ plants demonstrated that 72 plants were susceptible，with symptoms similar to those of the susceptible variety Bingtang sweet orange，118 slightly less susceptible than Bingtang sweet orange，but similar to those of the susceptible parent Red pomelo；10 were resistant，similar to the resistant parent American citron and four were resistant as the resistant genotype citron C-05. In addition，the symptoms of 21 F₂ plants showed different reaction following the inoculation with Xcc between the adaxial and abaxial leaf surface. On 10 plants，typical canker symptoms appeared on the adaxial leaf surface but not on the abaxial one；while for the other 11 plants，the typical symptoms inversely emerged on the abaxial leaf surface and not on the adaxial leaf surface. The quantification results of Xcc on the inoculated leaves was consistent with the of in vivo identification. In summary，no segregation of resistance and susceptibility was observed in F₁ progeny，but did in the F₂ generation. The ratio of resistant to susceptible F₂ plant was 1︰15，indicating that resistance and susceptibility may be inherited as quantitative traits.

Key words: citron, Red pomelo, F₂ plant, citrus canker disease, resistance identification, quantification of Xcc

CLC Number:

S666

WANG Ping, SHENG Ling, YANG Jinpeng, ZHOU Linglei, JIN Yan, LUO Xuzhao, MA Xianfeng, DENG Ziniu. Evaluation of Resistance to Citrus Canker Disease in Hybrid Progeny of Red Pomelo and American Citron[J]. Acta Horticulturae Sinica, 2023, 50(4): 765-777.

Figures/Tables 9

Fig. 1 Schematic diagram of in vivo inoculation of Xcc

Fig. 2 F2 citrus live leaves are graded and represent individual strains after inoculation with canker bacteria

Fig. 3 Symptoms at 20 d after injection of single live leaf of F1 part of Red pomelo and American citron （105 and 104 cfu · mL-1）with ulcerative pathogen

Fig. 4 Symptoms of single isolated leaves of hybrid F2 of Red pomelo and American citron inoculated with ulcerative pathogen（105，106 and 107 cfu · mL-1）at 10 d

Table 1 Classification of canker disease sumptoms following in vivo inoculation of F2 plants of Red pumelo × American citron

F₁ 单株 F₁ plant	植株数 Number of plants
F₁ 单株 F₁ plant	F₂	等级1 Grade Ⅰ	等级2 Grade Ⅱ	等级3 Grade Ⅲ	等级4 Grade Ⅳ	叶正面极感 Adaxial leaf surface sensation	叶反面极感 Abaxial leaf surface serious
E48	156	3	5	70	46	5	9
E73	63	1	1	31	23	1	1
E65	16	—	22	7	—	4	—
E5	18	—	2	10	3	—	1
总计（株） Total（plant）	253	4	10	118	72	10	11

Fig. 5 Symptoms（20 d）of single living leaves of the F2 part of a hybrid of Red pumelo × American citron inoculated with ulcerative bacteria（105 and 104 cfu · mL-1）

Fig. 6 Symptoms of large difference between the front and back sides of the leaves（20 d）of a single plant of F2 hybridization of Red pumelo × American citron inoculated with ulcerative bacteria（105 and 104 cfu · mL-1）

Fig. 7 Growth of ulcerative bacteria after inoculation with different resistant germplasm and some F2 plant leaves BTC：‘Bingtang’sweet orange；HXY：Red pommelo；JYC05：Citron C-05；MGJY：American citron. Different lowercase letters indicate signicant differences in the content of ulcerative bacteria between different leaves（P < 0.05）.

Table 2 Summary of the resistant and susceptle F2 plants of Red pomelo × American citron

F₁单株 F₁plant	植株数 Number of plants
F₁单株 F₁plant	F₂	活体鉴定 Identified	抗病株 Resistant plant	感病株Susceptible plant
E48	156	138	8	130
E73	63	58	2	56
E65	16	13	2	11
E5	18	16	2	14
总计Total	253	225	14	211

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Evaluation of Resistance to Citrus Canker Disease in Hybrid Progeny of Red Pomelo and American Citron

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