Abstract: Making Lomatogonium rotatum（L.）Fries ex Nym. as material，the floral organ characteristics，flowering dynamics，and pollination insects were studied by field observation. The pollen vigor，pollination of stigma，the ratio of pollen and ovule（P/O），hybridization index（OCI），and artificial pollination were used to test the propagation system. The results showed that the single flowering stage was 6–7 d，including single flower bud stage，early stage，blooming stage，decadence stage and fade stage. The stigma always exceeded anther during the whole flowering process. The pollen viability and stigma receptivity of 2–3 d after flowering were stronger. The ratio of pollen and ovule（P/O）is 551.02，and the hybridization index is 4. The main breeding system is heterosynthesis，partial self-compatibility，and thrips as the main pollination medium. The seedling rate of the fruit was 0 after emasculation with bags and no pollination，indicating that there was no apomixis.

Key words: Lomatogonium rotatum, floral organ characteristics, flowering dynamics, breeding system