Abstract:

Inducing tetraploid plants is an effective way to overcome the infertility of distant hybridization. In the present paper，tetraploid inducing and identification of Brassica nigra were conducted. The results showed that treating the seedling apexes of B. nigra with 0.1% colchicine solution for 24–72 h，the tetraploid plants were induced，in which the treatment for 48 hours achieved the highest percentage（73.33%）of tetraploid plants. The tetraploid plants grew and developed normally，their blades and flowers were obviously larger than those of diploid plants. The anthesis of the tetraploid plants was about
2–3 days later than the diploid plants. The seed setting of tetraploid plants was only 13.83%.

Key words: Brassica nigra, autotetraploid, colchicine