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ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (8): 1527-1534.

• Ornamental Plants • Previous Articles     Next Articles

Dynamic Changes of Starch Granules During Somatic Embryogenesis and Development in Cyclamen persicum

YU Wen-sheng1,JIANG Wei1,GONG Xue-qin2,LUO Zhen-zhen1,XIE Xiao-xu1,and YOU Cui-rong2,*   

  1. (1Garden Management Department of Yantai,Yantai,Shandong 264000,China;2College of Life Science,Yantai University,Yantai,Shandong 264005,China)
  • Received:2013-04-12 Online:2013-08-25 Published:2013-08-25

Abstract: Embryogenic calluses were induced and filtrated from young leaf explants in flowering Cyclamen persicum plant,and somatic embryos were formed. Histological observation revealed that embryogenic calluses were comprised of embryogenic and non-embryogenic cells,and that embryogenic cells mostly existed in embryogenic aggregates. Embryogenic aggregates derived from induced embryogenic determined cells. Somatic embryogenesis originated from a single embryogenic cell. Somatic embryo grew into a complete plantlet after passing through proembryos,globular,heart- and torpedo- shaped stages. During somatic embryogenesis and development,four peaks of starch granules appeared,respectively at the stage of embryogenic cells,globular embryos,early torpedo-shaped embryos and complete plantlets from mature embryos. Starch metabolism was closely related to somatic embryogenesis,development and plantlet regeneration.

Key words: Cyclamen persicum, somatic embryo, induced embryogenic determined cell, starch granule