Abstract: A forward subtractive cDNA library was constructed through suppression subtractive hybridization（SSH） method with mirror orientation selection，which was performed using the cDNA from wild type‘Guanxi’pomelo（Citrus grandis）as driver，those from its red-flesh mutant as tester. PCR analysis showed that most of the clones with insertions ranged from 100 bp to 1 000 bp. After differential screening through dot hybridization，a total of 102 transcripts represented 44 Unigenes with signal ratios stronger than two times were selected for 2 additional screenings. Homology analysis showed that they
were found to be involved in diverse biological processes，such as signal transduction，protein synthesis，stress response as well as transport metabolism.

Key words: words：Citrus grandis（L.）Osbeck, mutant strain, suppression subtractive hybridization（SSH）, differentially expressed sequence