Cloning and Functional Analysis of OguCMS-related gene BoMF1 Promoter in Brassica oleracea

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (5): 887-.

• Vegetables • Previous Articles Next Articles

Cloning and Functional Analysis of OguCMS-related gene BoMF1 Promoter in Brassica oleracea

GUO Ying-Ying, JIE Jian-Ming, JIAN Yuan-Cai, YU Ji-Hua, KANG Jun-Gen

1Gansu Agriculture University，Lanzhou 730070，China；2Beijing Vegetable Research Center，Beijing Academy of
Agriculture and Forestry Sciences，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（North
China），Ministry of Agriculture，Beijing 100097，China

Online:2013-05-25 Published:2013-05-25

Abstract

Abstract: The regulative sequence（521 bp）of OguCMS-related gene BoMF1 promoter from
Brassica oleracea was cloned by genomic walking（TAIL-PCR）. In silico analysis showed that this
sequence contained several acting elements，including TATA-box and CAAT-box，MYB binding sites，
phytohormone responsive elements and so on. In order to study the promoter function，a 521 bp promoter
sequence of BoMF1 was inserted upstream of the GUS reporter gene replacing the CaMV35S promoter of
pBI121. The plant expression vector pBI121-BoMF1P and pBI121 were transformed into Arabidopsis
thaliana with the Agrobacterium tumefaciens strain LBA4404. It was suggested that pBI121-BoMF1P
could drive the GUS gene exclusively express in anther and pollen of Arabidopsis thaliana.

Key words: Brassica oleracea, OguCMS, BoMF1 promoter, GUS, cis-element

CLC Number:

S 635.1

GUO Ying-Ying, JIE Jian-Ming, JIAN Yuan-Cai, YU Ji-Hua, KANG Jun-Gen. Cloning and Functional Analysis of OguCMS-related gene BoMF1 Promoter in Brassica oleracea[J]. ACTA HORTICULTURAE SINICA, 2013, 40(5): 887-.

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Cloning and Functional Analysis of OguCMS-related gene BoMF1 Promoter in Brassica oleracea

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