Abstract: Tube-flower is a novel form of in vitro plant products. A highly efficient in vitro
regeneration system was established for the production of succulent plant Crassula clavata N. E. Brown.
In primary culture，adventitious buds instead of adventitious roots were differentiated from the shoot
explants on MS medium supplemented with different concentrations of IBA；The highest multiplication
rate and high-quality adventitious buds were obtained on the optimal medium of MS containing 0.3
mg · L-1 IBA. In subculture，the medium of MS containing 0.1 mg · L-1 NAA and 0.5 mg · L-1 6-BA was
screened out for large amounts of embryogenic callus could be significantly induced and sprouts could be
further differentiated on the callus surface. In culture for colorful decorative products，0.25 mg · L-1 edible
colourant to half strength MS medium was feasible to give pleasant visual effect without harmful or toxic
effect on the in vitro plants.

Key words: Crassula clavata N. E. Brown, micro-propagation, tube-flower, regeneration system