Abstract: The study has transformed the cold resistant gene rd29A into the acceptor，the embryo
callus derived from Photinia fraseri leaf，in order to establish the transformation system by using particle
bombardment method. The results indicate that the optimal transformation scheme for Photinia fraseri leaf
callus using particle bombardment method was treating embryogenic callus tissue within 0.3 mol · L-1
mannitol for 15–16 h，in MS medium supplemented with 2.0 mg · L-1 6-benzy aminopurine，5.0 mg · L-1
napthaleneacetic acid，0.5 mg · L-1 2,4-dichlorophenoxyacetic acid，5.0 g · L-1 agar powder and 20 g · L-1
glucose. When particle bombardmenting，in addition of 12.5 mol · L-1 CaCl2 50 μL and 0.1 mol · L-1
spermidine 50 μL，and bombarding the samples once a time，at 9 cm bombardment distance，at 1 100 psi
heli μm pressure and with the dosage of gold powder 300 μg and plasmid DNA 3.0 μg per bombardment.
Six transgenic Photinia fraseri plants were obtained in the study and it was confirmed that the rd29A gene
was integrated into Photinia fraseri genomes according to the PCR and Southern analysis of plant DNA.

Key words: Photinia fraseri, rd29A, microprojectile bombardment, transgenic plants