Abstract: Thirteen fingered citron accessions，1 citrate acid orange and 6 citron accessions were
collected and fourteen genotypes were introduced and survived in the germplasm resources garden. Based
on the biological traits，the genetic diversities of 20 samples were evaluated by ISSR markers. A total of
102 DNA bands were amplified from the 11 polymorphic primers，which were screened from 100 ISSR
primers. Among those obtained bands，58 were polymorphic ones，accounting for 56.9% polymorphism.
The genetic similarity coefficient among the accessions ranged from 0.608 to 0.990. At the point of the
similarity coefficient 0.798，all the accessions might be separated into three groups，fingered citron，citrate
acid orange and citron，meanwhile three pieces of Yabian mutants belonged to a new group of fingered
citron.