Abstract: The large ornamental plant，Platycerium wallichii Hook.，was investigated with both axenic cultivation and soil medium to study its spore culture and rejuvenation. The results indicated that the optimal temperature of spore germination are about 20–30 ℃. Spores did not germinate in dark conditions，and the optimum light intensity for growth are 60–80 μmol · m^-2 · s^-1. The successful development of gametophyte was achieved with the pH 4.5 to 7.5. It is more suitable for spore germination and prothallus to formation with 2% or lower sucrose. When the young sporophytes reach 2–3 cm length，it can be transplanted. The young sporophyte can be cultivateded in the mixed medium composed of leaf mould substrate，sand and organic fertilizer. Young sporophyte shows somewhat ornamental values when it has 10 cm height.

Key words: Platycerium wallichii Hook, spore, sexual propagation