The Technology System Establishment of VIGS in Non-heading Chinese Cabbage

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (11): 2168-2174.

• Vegetables • Previous Articles Next Articles

The Technology System Establishment of VIGS in Non-heading Chinese Cabbage

YANG Xue-Dong, DAI Wei, ZHANG Chang-Wei, WANG Jin-Yan, KONG Min, HOU Xi-Lin

（State Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing Agricultural University；Key Laboratory of Biology and Genetic Improvement of Horticulural Crops（East China），Ministry of Agriculture，Nanjing 210095，China）

Online:2012-11-25 Published:2012-11-25

Abstract

Abstract: In this study，a viral vector derived from Turnip yellow mosaic virus（TYMV）was used to induce virus-induced gene silencing（VIGS）of phytoene desaturase gene（PDS） in non-heading Chinese cabbage（Brassica campestris ssp. chinensis Makino.）cultivar‘Suzhouqing’and the result showed that the expression of PDS was successfully repressed. The ability of inducing silence was confirmed and the VIGS system was established. To further confirm the VIGS effect on non-heading Chinese cabbage，BcNRT1 was selected as the target gene，and the expression of BcNRT1 was successfully inhibited after inoculated.

Key words: non-heading Chinese cabbage, Turnip yellow mosaic virus, virus-induced gene silencing

CLC Number:

S634.3

YANG Xue-Dong, DAI Wei, ZHANG Chang-Wei, WANG Jin-Yan, KONG Min, HOU Xi-Lin. The Technology System Establishment of VIGS in Non-heading Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2012, 39(11): 2168-2174.

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The Technology System Establishment of VIGS in Non-heading Chinese Cabbage

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