Abstract: For further study on the interaction of SRK-SCR and between these two determinant factors，Brassica oleracea with high self-incompatibility was used as research materials to construct a group of pGADT7-eSRKs and pGBKT7-SCRs recombinant vectors that were subsequently transformed accordingly into Y187 and Y2HGold yeast. The interaction between these two recombinant plasmids transformed into the yeasts were tested by SD/-Ade-His-Trp-Leu fungal colony formation of plates，PCR and x-α-gal color reactions. The results showed that there existed two kinds of interactions among all interaction assays，which preliminary indicated that their interaction region span 16–421 bp fragment of the first exon of SRK and 1 876–2 068 bp fragment of SCR. These results reconfirm the SRK-SCR interaction，adding some insights into the mechanism of selt incompatibility in Brassica.

Key words: Brassica oleracea, SRK, eSRKs gene, SCRs gene, pGADT7 vector, pGBKT7 vector, yeast two-hybrid system