Abstract: Uni-ESTs of Moso bamboo（Phyllostachys edulis）from the database of dbEST were screened using Websat software and 408 SSRs in 401 ESTs were mined out，accounting for 13.02% of the total ESTs，corresponding to one SSR in every 6.8 kb of the ESTs. Dinucleotide and trinucleotide repeats EST-SSRs were dominant，accounting for 43.14% and 49.57% respectively. (AG)n is the most frequent motif in dinucleotide repeats，accounting for 37.01% in total EST-SSRs，followed by (TC)n，(AGC)n，(GAG)n and (CGC)n. 182 primer pairs were designed which 42 of them showed amplifications，and 39 primer pairs could have products with polymorphisms from 12 different bamboo samples. The results indicated that it is an available approach to develop EST-SSR markers based on ESTs from Moso bamboo.

Key words: Moso bamboo, Phyllostachys edulis, EST-SSR